Results 61 to 70 of about 336,180 (306)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Macrophage-induced reduction of bacteriophage density limits the efficacy of in vivo pulmonary phage therapy

Nature Communications
The rise of antimicrobial resistance leads to renewed interest in evaluating phage therapy. In murine models highly effective treatment of acute pneumonia caused by Pseudomonas aeruginosa relies on the synergistic antibacterial activity of bacteriophages
Sophia Zborowsky, Jérémy Seurat, Quentin Balacheff, Solène Ecomard, Céline Mulet, Chau Nguyen Ngoc Minh, Marie Titécat, Emma Evrard, Rogelio A. Rodriguez-Gonzalez, Jacopo Marchi, Joshua S. Weitz, Laurent Debarbieux   +11 more
doaj   +1 more source

The Appearance of Antiphage Antibodies in Sera of Patients Treated with Phages

Antibiotics
Background: Bacteriophages are neutralized by the sera of patients undergoing phage therapy (PT), particularly during local or concomitant local and oral phage administration in bone infections, soft tissue infections, or upper respiratory tract ...
Marzanna Łusiak-Szelachowska, Beata Weber-Dąbrowska, Maciej Żaczek, Ryszard Międzybrodzki, Andrzej Górski   +4 more
doaj   +1 more source

Specific metaphase and interphase detection of the breakpoint region in 8q24 of burkitt lymphoma cells by triple-color fluorescence in situ hybridization [PDF]

, 1992
Triple fluorescence in situ hybridization with a plasmid DNA library from sorted human chromosomes 8 in combination with bacteriophage clones flanking the breakpoint in 8q24 of the Burkitt lymphoma cell line Jl was used for the specific delineation of ...
Anastasi, Arnoldus, Baldini, Bornkamm, Cremer, Cremer, Cremer, Cremer, Crescenzi, Devilee, Emmerich, Hartl, Heim, Henglein, Hopman, Hopman, Klobeck, Kolluri, Lee, Lengauer, Lengauer, Lichter, Lichter, Lichter, Maniatis, Manolov, Nederlof, Nederlof, Pinkel, Rappold, Tkachuk, Walt, Wiegant, Zech   +33 more
core   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

A Thorough Synthesis of Phage Therapy Unit Activity in Poland—Its History, Milestones and International Recognition

Viruses, 2022
The year 2020 marked 15 years of the Phage Therapy Unit in Poland, the inception of which took place just one year after Poland’s accession to the European Union (2004).
Maciej Żaczek, Andrzej Górski, Beata Weber-Dąbrowska, Sławomir Letkiewicz, Wojciech Fortuna, Paweł Rogóż, Edyta Pasternak, Ryszard Międzybrodzki   +7 more
doaj   +1 more source

Bacteriophages as an Up-and-Coming Alternative to the Use of Sulfur Dioxide in Winemaking [PDF]

, 2020
Certain acetic and lactic acid bacteria are major causes of quality defects in musts and wines, giving rise to defects such as a "vinegary," "sharp, like nail polish-remover" taste or preventing alcoholic and/or malolactic fermentation. Sulfur dioxide is
Cantoral Fernández, Jesús Manuel, Cordero Bueso, Gustavo Adolfo, Moraga Galindo, Javier, Ruiz Muñoz, Marina, Ríos Carrasco, María   +4 more
core   +2 more sources

Bacteriophage genomics [PDF]

Current Opinion in Microbiology, 2008
The past three years have seen an escalation in the number of sequenced bacteriophage genomes with more than 500 now in the NCBI phage database, representing a more than threefold increase since 2005. These span at least 70 different bacterial hosts, with two-thirds of the sequenced genomes of phages representing only eight bacterial hosts.
openaire   +2 more sources

Dual‐Layer Living Hydrogel Enables On‐Demand Delivery of Phages and Probiotics for Synergistic Wound Infection Therapy

Advanced Functional Materials, EarlyView.
A dual‐layer living hydrogel, ProΦGel, integrates bacteriophages and probiotics for synergistic wound infection therapy. The outer gelatin‐based matrix releases phages on demand in response to P. aeruginosa infections, while inner alginate beads sustain probiotic delivery.
Siyuan Tao, Darine D'Adam, Anna Hu, Sixuan Zhang, Josua Roduner, Katharina Maniura‐Weber, Qun Ren   +6 more
wiley   +1 more source

Selection of turning-on fluorogenic probe as protein-specific detector obtained via the 10BASEd-T [PDF]

, 2017
In order to obtain a molecular probe for specific protein detection, we have synthesized fluorogenic probe library of vast diversity on bacteriophage T7 via the gp10 based-thioetherification (10BASEd−T).
Masumi Taki, Shuta Uematsu, Taiki Midorikawa, Yuji Ito   +3 more
core   +2 more sources