Results 71 to 80 of about 355,018 (412)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Highly Sensitive Bacteriophage-Based Detection of Brucella abortus in Mixed Culture and Spiked Blood

Viruses, 2017
For decades, bacteriophages (phages) have been used for Brucella species identification in the diagnosis and epidemiology of brucellosis. Traditional Brucella phage typing is a multi-day procedure including the isolation of a pure culture, a step that ...
Kirill V. Sergueev, Andrey A. Filippov, Mikeljon P. Nikolich   +2 more
doaj   +1 more source

Structural Studies on Bacteriophage Portal Proteins [PDF]

, 2013
In tailed bacteriophages and evolutionarily related herpes viruses, the portal protein is a central component of the DNA packaging molecular motor, which translocates viral genomic DNA into a preformed procapsid.
Luan, Weisha
core  

Targeting Pseudomonas aeruginosa biofilm with an evolutionary trained bacteriophage cocktail exploiting phage resistance trade-offs

Nature Communications
Spread of multidrug-resistant Pseudomonas aeruginosa strains threatens to render currently available antibiotics obsolete, with limited prospects for the development of new antibiotics.
Fabian Kunisch, Claudia Campobasso, J. Wagemans, S. Yildirim, B. Chan, Christoph Schaudinn, R. Lavigne, P. E. Turner, Michael J. Raschke, Andrej Trampuz, M. Gonzalez Moreno   +10 more
semanticscholar   +1 more source

Molecular and Evolutionary Determinants of Bacteriophage Host Range.

Trends in Microbiology, 2019
The host range of a bacteriophage is the taxonomic diversity of hosts it can successfully infect. Host range, one of the central traits to understand in phages, is determined by a range of molecular interactions between phage and host throughout the ...
P. A. de Jonge, Franklin L. Nobrega, Stan J. J. Brouns, B. Dutilh   +3 more
semanticscholar   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Staphylococcus aureus temperate bacteriophage: carriage and horizontal gene transfer is lineage associated.

, 2012
Staphylococcus aureus is a major cause of human and animal infections. Bacteriophage are a class of mobile genetic element (MGE) that carry virulence genes and disseminate them horizontally, including Panton-Valentine leukocidin (PVL), the immune evasion
McCarthy, AJ, Lindsay, JA, Alex J Mccarthy, Jodi A Lindsay, Witney, AA, Adam A Witney, Alex J. McCarthy, Jodi. A. Lindsay, Adam A. Witney   +8 more
core   +1 more source

From DNA sequence to application: possibilities and complications [PDF]

, 1999
The development of sophisticated genetic tools during the past 15 years have facilitated a tremendous increase of fundamental and application-oriented knowledge of lactic acid bacteria (LAB) and their bacteriophages.
Venema, Gerard,, van Sinderen, D, Sinderen, Douwe van,, Kok, Jan,, Kok, J, Gerard Venema, Douwe van Sinderen, Venema, G, Jan Kok   +8 more
core   +1 more source

Emerging roles of bacteriophage-based therapeutics in combating antibiotic resistance

Frontiers in Microbiology
Amid the growing challenge of antibiotic resistance on a global scale, there has been a notable resurgence in bacteriophage-based treatments, signaling a shift in our approach to managing infections.
Anandhalakshmi Subramanian
semanticscholar   +1 more source

Bacteriophage therapy against Pseudomonas aeruginosa biofilms: a review

Annals of Clinical Microbiology and Antimicrobials, 2020
Multi-Drug Resistant (MDR) Pseudomonas aeruginosa is one of the most important bacterial pathogens that causes infection with a high mortality rate due to resistance to different antibiotics.
Z. Chegini, Amin Khoshbayan, Majid Taati Moghadam, Iman Farahani, Parham Jazireian, A. Shariati   +5 more
semanticscholar   +1 more source