Results 81 to 90 of about 285,810 (297)

A Thorough Synthesis of Phage Therapy Unit Activity in Poland—Its History, Milestones and International Recognition

Viruses, 2022
The year 2020 marked 15 years of the Phage Therapy Unit in Poland, the inception of which took place just one year after Poland’s accession to the European Union (2004).
Maciej Żaczek, Andrzej Górski, Beata Weber-Dąbrowska, Sławomir Letkiewicz, Wojciech Fortuna, Paweł Rogóż, Edyta Pasternak, Ryszard Międzybrodzki   +7 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

vB_BcM_Sam46 and vB_BcM_Sam112, members of a new bacteriophage genus with unusual small terminase structure

Scientific Reports, 2021
One of the serious public health concerns is food contaminated with pathogens and their vital activity products such as toxins. Bacillus cereus group of bacteria includes well-known pathogenic species such as B. anthracis, B. cereus sensu stricto (ss), B.
Olesya A. Kazantseva, Emma G. Piligrimova, Andrey M. Shadrin   +2 more
doaj   +1 more source

Salmonella enterica serovar typhi limits the potency of typhoid toxin and ADP‐ribosylating toxin AB to establish a persistent infection

FEBS Open Bio, EarlyView.
The two catalytic subunits of typhoid toxin dissociate from the holotoxin in the ER of an intoxicated cell, but only CdtB exits the ER to generate immunosuppressive effects. PltA is retained in the ER and sequestered from its cytosolic target, thus allowing the anti‐inflammatory effects of CdtB to promote intestinal colonization.
Maria C. Zabala‐Rodriguez, Juliette K. Tinker, Teresa Frisan, Taj Azarian, Ken Teter   +4 more
wiley   +1 more source

Comprehensive Approaches to Combatting Acinetobacter baumannii Biofilms: From Biofilm Structure to Phage-Based Therapies

Antibiotics
Acinetobacter baumannii—a multidrug-resistant (MDR) pathogen that causes, for example, skin and soft tissue wounds; urinary tract infections; pneumonia; bacteremia; and endocarditis, particularly due to its ability to form robust biofilms—poses a ...
Ilona Grygiel, Olaf Bajrak, Michał Wójcicki, Klaudia Krusiec, Ewa Jończyk-Matysiak, Andrzej Górski, Joanna Majewska, Sławomir Letkiewicz   +7 more
doaj   +1 more source

Conformational Switching of Proteins on Material Surfaces Enabled by Peptide‐Oriented Adsorption

Advanced Functional Materials, EarlyView.
Responsive biointerfaces could be achieved using conformationally responsive proteins; however, their interconversion can be lost upon surface adsorption. Herein, we demonstrate that the incorporation a Au binding peptide into calmodulin allows for binding control on Au wherein the protein retains conformational switching. This capability was confirmed
Sakthirupini Ramamurthy, Kyle B. Meerbott, Lorenzo Travaglini, Garima S. Dobhal, Artur Sawicki, Marc R. Knecht, Tiffany R. Walsh, Dominic J. Glover   +7 more
wiley   +1 more source

Sequencing and analysis of the DNA genome of the temperate bacteriophage Aa[Phi]23 of "Actinobacillus actinomycetemcomitans" [PDF]

, 2005
The entire genomic sequence of the AaΦ23 bacteriophage is presented. Its size is 43,033 bp and it has an overall molar G+C content of 41mol%. 57 potential open reading frames (ORFs) were identified.
Resch, Grégory
core   +1 more source

Nanoamphizymes: Self‐Assembled Amphiphilic Enzymatic Nanomaterials for High‐Performance Biocatalysis and Biosensing

Advanced Functional Materials, EarlyView.
An amphiphilic GOx nanoamphizyme was constructed by covalently introducing hydrophobic PPNI linkers onto GOx, enabling spontaneous self‐assembly into tunable nanoparticles. Mild crosslinking further stabilized the assemblies and enhanced catalytic performance and stability.
Yuan‐Hao Hsiao, Rajan Deepan Chakravarthy, Po‐Hsiang Chen, Abdelreheem Abdelfatah Saddik, Hsin‐Chieh Lin   +4 more
wiley   +1 more source