Results 81 to 90 of about 355,018 (412)

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Macrophage-induced reduction of bacteriophage density limits the efficacy of in vivo pulmonary phage therapy

Nature Communications
The rise of antimicrobial resistance leads to renewed interest in evaluating phage therapy. In murine models highly effective treatment of acute pneumonia caused by Pseudomonas aeruginosa relies on the synergistic antibacterial activity of bacteriophages
Sophia Zborowsky, Jérémy Seurat, Quentin Balacheff, Solène Ecomard, Céline Mulet, Chau Nguyen Ngoc Minh, Marie Titécat, Emma Evrard, Rogelio A. Rodriguez-Gonzalez, Jacopo Marchi, Joshua S. Weitz, Laurent Debarbieux   +11 more
doaj   +1 more source

The Appearance of Antiphage Antibodies in Sera of Patients Treated with Phages

Antibiotics
Background: Bacteriophages are neutralized by the sera of patients undergoing phage therapy (PT), particularly during local or concomitant local and oral phage administration in bone infections, soft tissue infections, or upper respiratory tract ...
Marzanna Łusiak-Szelachowska, Beata Weber-Dąbrowska, Maciej Żaczek, Ryszard Międzybrodzki, Andrzej Górski   +4 more
doaj   +1 more source

Putative plasmid prophages of Bacillus cereus sensu lato may hold the key to undiscovered phage diversity

Scientific Reports, 2021
Bacteriophages are bacterial viruses and the most abundant biological entities on Earth. Temperate bacteriophages can form prophages stably maintained in the host population: they either integrate into the host genome or replicate as plasmids in the host
Emma G. Piligrimova, Olesya A. Kazantseva, Andrey N. Kazantsev, Nikita A. Nikulin, Anna V. Skorynina, Olga N. Koposova, Andrey M. Shadrin   +6 more
doaj   +1 more source

What Are the Potential Benefits of Using Bacteriophages in Periodontal Therapy?

Antibiotics, 2022
Periodontitis, which may result in tooth loss, constitutes both a serious medical and social problem. This pathology, if not treated, can contribute to the development of, among others, pancreatic cancer, cardiovascular diseases or Alzheimer’s disease ...
Jan Kowalski, Renata Górska, Martyna Cieślik, Andrzej Górski, Ewa Jończyk-Matysiak   +4 more
doaj   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

Bacteriophage Therapy for Critical Infections Related to Cardiothoracic Surgery

Antibiotics, 2020
(1) Objective: Bacterial resistance to conventional antibiotic therapy is an increasingly significant worldwide challenge to human health. The objective is to evaluate whether bacteriophage therapy could complement or be a viable alternative to ...
Evgenii Rubalskii, S. Ruemke, C. Salmoukas, Erin C Boyle, G. Warnecke, I. Tudorache, M. Shrestha, J. Schmitto, A. Martens, S. Rojas, S. Ziesing, S. Bochkareva, C. Kuehn, A. Haverich   +13 more
semanticscholar   +1 more source

Bacteriophage genomics [PDF]

Current Opinion in Microbiology, 2008
The past three years have seen an escalation in the number of sequenced bacteriophage genomes with more than 500 now in the NCBI phage database, representing a more than threefold increase since 2005. These span at least 70 different bacterial hosts, with two-thirds of the sequenced genomes of phages representing only eight bacterial hosts.
openaire   +2 more sources

Bacteriophage Assembly [PDF]

Viruses, 2011
Bacteriophages have been a model system to study assembly processes for over half a century. Formation of infectious phage particles involves specific protein-protein and protein-nucleic acid interactions, as well as large conformational changes of assembly precursors.
Anastasia A. Aksyuk, Michael G. Rossmann
openaire   +3 more sources

Co‐Electrospinning Extracellular Matrix with Polycaprolactone Enables a Modular Approach to Balance Bioactivity and Mechanics of a Multifunctional Bone Wrap

Advanced Healthcare Materials, EarlyView.
The incorporation of nondigested ECM and synthetic polymers into a co‐electrospinning system enables the decoupling of bioactivity and mechanical properties within a single wrap. This technique is used to develop a multifunctional bone wrap that achieves augmented membrane durability, sustained infection control, and enhanced vascularity for use in ...
Sarah Jones, Madeline Laude, Zeenat Oyebanji, Ruchi Birur, Andres Luengo Martinez, Isabelle Gilbert, Phillip Baek, Nichole Longbottom, Nicole McCullough, Staci J. Horn, Chien‐Yu Lin, Matthew J. Lohr, Fred Clubb, Manuel K. Rausch, Elizabeth Cosgriff‐Hernandez   +14 more
wiley   +1 more source