Results 21 to 30 of about 40,087 (166)

ATP-Dependent Mismatch Recognition in DNA Replication Mismatch Repair

Quantum Reports, 2023
Mismatch repair is a critical step in DNA replication that occurs after base selection and proofreading, significantly increasing fidelity. However, the mechanism of mismatch recognition has not been established for any repair enzyme.
Nianqin Zhang, Yongjun Zhang
doaj   +1 more source

A unified Watson-Crick geometry drives transcription of six-letter expanded DNA alphabets by E. coli RNA polymerase

Nature Communications, 2023
Artificially Expanded Genetic Information Systems (AEGIS) add independently replicable unnatural nucleotide pairs to the natural G:C and A:T/U pairs found in native DNA, joining the unnatural pairs through alternative modes of hydrogen bonding.
Juntaek Oh, Zelin Shan, Shuichi Hoshika, Jun Xu, Jenny Chong, Steven A. Benner, Dmitry Lyumkis, Dong Wang   +7 more
doaj   +1 more source

Unbranched rod-like RNA is required for RNA editing of hepatitis delta virus genotype 2 and genotype 4

Virus Research, 2023
RNA editing of the hepatitis delta virus (HDV) is essential for generating the large delta antigen, which is crucial for virion assembly. In HDV genotype 1 (HDV-1), editing occurs within the context of the unbranched rod-like structure characteristic of ...
Chao-Wei Hsu, Hsueh-Ying Hsu, Chien-Hung Chen, Mei Chao   +3 more
doaj   +1 more source

SNP Discrimination by Tolane-Modified Peptide Nucleic Acids: Application for the Detection of Drug Resistance in Pathogens

Molecules, 2020
During the treatment of viral or bacterial infections, it is important to evaluate any resistance to the therapeutic agents used. An amino acid substitution arising from a single base mutation in a particular gene often causes drug resistance in ...
Kenji Takagi, Tenko Hayashi, Shinjiro Sawada, Miku Okazaki, Sakiko Hori, Katsuya Ogata, Nobuo Kato, Yasuhito Ebara, Kunihiro Kaihatsu   +8 more
doaj   +1 more source

pH-Responsive DNA Motif: From Rational Design to Analytical Applications

Frontiers in Chemistry, 2021
pH-responsive DNA motifs have attracted substantial attention attributed to their high designability and versatility of DNA chemistry. Such DNA motifs typically exploit DNA secondary structures that exhibit pH response properties because of the presence ...
Lin Lin Zheng, Jin Ze Li, Ying Xu Li, Jian Bang Gao, Jiang Xue Dong, Zhong Feng Gao   +5 more
doaj   +1 more source

Sequence-Dependent T:G Base Pair Opening in DNA Double Helix Bound by Cren7, a Chromatin Protein Conserved among Crenarchaea. [PDF]

PLoS ONE, 2016
T:G base pair arising from spontaneous deamination of 5mC or polymerase errors is a great challenge for DNA repair of hyperthermophilic archaea, especially Crenarchaea.
Lei Tian, Zhenfeng Zhang, Hanqian Wang, Mohan Zhao, Yuhui Dong, Yong Gong   +5 more
doaj   +1 more source

Optimization of Single-Base-Pair Mismatch Discrimination in Oligonucleotide Microarrays [PDF]

Applied and Environmental Microbiology, 2003
ABSTRACT The discrimination between perfect-match and single-base-pair-mismatched nucleic acid duplexes was investigated by using oligonucleotide DNA microarrays and nonequilibrium dissociation rates (melting profiles).
Urakawa, H., El Fantroussi, S., Smidt, H., Smoot, J.C., Tribou, E.H., Kelly, J.J., Noble, P.A., Stahl, D.A.   +7 more
openaire   +4 more sources

Dissociation kinetics of 19 base paired oligonucleotide-DNA duplexes containing different single mismatched base pairs [PDF]

Nucleic Acids Research, 1987
The dissociation kinetics of 19 base paired oligonucleotide-DNA duplex containing a various single mismatched base pair are studied on dried agarose gels. The kinetics of the dissociation are first order under our experimental conditions. The incorporation of a single mismatched base pair destabilizes the DNA duplexes to some extent, the amount ...
S, Ikuta, K, Takagi, R B, Wallace, K, Itakura   +3 more
openaire   +2 more sources

Mismatched DNA Base Pairs Show Increased Conformational Fluctuations [PDF]

Biophysical Journal, 2015
Base pair mismatches in DNA can have many adverse consequences, yet the exact mechanism by which mismatches are repaired is unknown. Both matched and mismatched DNA sequences were studied using molecular dynamics in biased and unbiased simulation. Significant differences were found between matched and mismatched pairs in structure, hydrogen bonding ...
Kingsland, Adelaide, Maibaum, Lutz
openaire   +1 more source

Dependence of Fluorescence Quenching of CY3 Oligonucleotide Conjugates on the Oxidation Potential of the Stacking Base Pair

Molecules, 2020
To understand the complex fluorescence properties of astraphloxin (CY3)-labelled oligonucleotides, it is necessary to take into account the redox properties of the nucleobases.
Jens Sobek, Ralph Schlapbach
doaj   +1 more source