Results 51 to 60 of about 40,087 (166)
Cell Reports, 2015 CRISPR-Cas systems provide bacteria and archaea with adaptive immunity against foreign nucleic acids. In type I CRISPR-Cas systems, invading DNA is detected by a large ribonucleoprotein surveillance complex called Cascade.
Marius Rutkauskas +5 more
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Anthracene-modified oligonucleotides as fluorescent DNA mismatch sensors: discrimination between various base-pair mismatches [PDF]
Supramolecular Chemistry, 2011 A fluorescent anthracene-tagged DNA probe has been shown to respond to various DNA sequences by changes to its emission signal upon duplex formation. The fluorescence response for duplexes containing a single mismatch near the anthracene site has been found to be very sensitive to its composition, with the emission signal increasing for a CA mismatch ...
Duprey, J.L.H.A. +8 more
openaire +2 more sources
A rule of seven in Watson-Crick base-pairing of mismatched sequences [PDF]
Nature Structural & Molecular Biology, 2012 Sequence recognition through base-pairing is essential for DNA repair and gene regulation, but the basic rules governing this process remain elusive. In particular, the kinetics of annealing between two imperfectly matched strands is not well characterized, despite its potential importance in nucleic acid-based biotechnologies and gene silencing.
Cisse, Ibrahim I. +2 more
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Detection of base-pair mismatches in DNA using graphene-based nanopore device [PDF]
Nanotechnology, 2016 Accepted in Nanotechnology, 12 pages, 7 ...
Kundu, Sourav, Karmakar, S. N.
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BMC Biotechnology, 2011 Background Many SNP discrimination strategies employ natural restriction endonucleases to discriminate between allelic states. However, SNPs are often not associated with a restriction site and therefore, a number of attempts have been made to generate ...
Chan Chee +2 more
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ZooKeys, 2017 The 137 individuals of Johnius grypotus were collected from seven localities from the Bohai Sea to the East China Sea. A 549 base pair (bp) fragment of the hypervariable region of the mtDNA control region was sequenced to examine genetic diversity and ...
Linlin Zhao +5 more
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Anharmonic Bending of DNA Base-Pair Mismatch [PDF]
Biophysical Journal, 2021 Michael L. Ryan +3 more
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Modeling DNA-Strand Displacement Reactions in the Presence of Base-Pair Mismatches
Journal of the American Chemical Society, 2020 Toehold-mediated strand displacement is the most abundantly used method to achieve dynamic switching in DNA-based nanotechnology. An "invader" strand binds to the "toehold" overhang of a target strand and replaces a target-bound "incumbent" strand. Here, the complementarity of the invader to the single-stranded toehold provides the free energy bias of ...
Patrick Irmisch +2 more
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Communications BiologyCRISPR/Cas12a is a highly promising detection tool. However, detecting single nucleotide variations (SNVs) remains challenging. Here, we elucidate Cas12a specificity through crRNA engineering and profiling of single- and double-base mismatch tolerance ...
Xujian Mao +12 more
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High-resolution structure of a DNA helix containing mismatched base pairs
Nature, 1985 The concept of complementary base pairing, integral to the double-helical structure of DNA, provides an effective and elegant mechanism for the faithful transmission of genetic information. Implicit in this model, however, is the potential for incorporating non-complementary base pairs (mismatches) during replication or subsequently, for example ...
Brown, T +3 more
openaire +3 more sources