Results 111 to 120 of about 7,909 (199)

Screening and validation of reference genes in Dracaena cochinchinensis using quantitative real-time PCR

Scientific Reports
Dragon's blood, the red resin derived from the wounded Dracaena, is a precious traditional medicine used by different culture. Dracaena cochinchinensis is one of the main species of Dracaena, and is the endangered medicinal plants in China.
Shixi Gao, Junxiang Peng, Mei Rong, Yang Liu, Yanhong Xu, Jianhe Wei   +5 more
semanticscholar   +1 more source

Cloning, Expression and Stability Analysis of Candidate Reference Genes in the Glacial Relict Hucho bleekeri Under Immune Stress

Journal of Applied Ichthyology, Volume 2025, Issue 1, 2025.
To find reference genes (RGs) and establish a screening method for RGs in Sichuan taimen (Hucho bleekeri), six candidate RGs—β-actin, b2m, β-tubulin, ef1α, gapdh and hprt — were cloned, and the stability of expression of the six candidate RGs were analysed. The six RG sequences in H.
Xiaoyun Wu, Jiansheng Lai, Yeyu Chen, Huanchao Yang, Yanling Chen, Zhao Liu, Quanyu Tu, Yi Yu, Hua Li, Poulami Jha   +9 more
wiley   +1 more source

Identification of Suitable Reference Genes for Gene Expression Normalization in Jatropha curcas L During Development and Under Stress Conditions Using Real Time Quantitative PCR

Brazilian Archives of Biology and Technology
Jatropha curcas L represent a potential source of raw material for the production of biodiesel. The aim this study was to find potential candidate reference genes in J. curcas tissues.
Antonio José Rocha, Paulo Abraão Maranhão, Rafaela Oliveira Silva, Simone Pohl, Cristiane S.R Fonteles   +4 more
doaj   +1 more source

Diploid and triploid Chinook salmon (Oncorhynchus tshawytscha) exhibit differential immunological responses to acute thermal stress

Journal of Fish Diseases, Volume 47, Issue 11, November 2024.
Abstract Exposure to temperatures outside of a fish's optimal range results in suppression of the immune system, ultimately leaving aquaculture stocks susceptible to disease outbreaks. This effect is exacerbated in triploid fishes, which demonstrate greater susceptibility to stress than their diploid counterparts. This study investigates the impacts of
Noah P. Rogozynski, Ivan G. Cadonic, Manuel Soto‐Dávila, Valentina Wong‐Benito, Tania Rodriguez‐Ramos, Paul Craig, Brian Dixon   +6 more
wiley   +1 more source

NormiRazor: tool applying GPU-accelerated computing for determination of internal references in microRNA transcription studies

BMC Bioinformatics, 2020
Background Multi-gene expression assays are an attractive tool in revealing complex regulatory mechanisms in living organisms. Normalization is an indispensable step of data analysis in all those studies, since it removes unwanted, non-biological ...
Szymon Grabia, Urszula Smyczynska, Konrad Pagacz, Wojciech Fendler   +3 more
doaj   +1 more source

Reference Genes Selection and Normalization of Oxidative Stress Responsive Genes upon Different Temperature Stress Conditions in Hypericum perforatum L [PDF]

, 2017
Reverse transcription-quantitative real-time PCR (RT-qPCR) is a widely used technique for gene expression analysis. The reliability of this method depends largely on the suitable selection of stable reference genes for accurate data normalization ...
Arnholdt-Schmitt, Birgit, Cardoso, Hélia, Ragonezi, Carla, Velada, Isabel   +3 more
core  

Selection of Reference Genes in Siraitia siamensis and Expression Patterns of Genes Involved in Mogrosides Biosynthesis

Plants
Siraitia siamensis is a traditional Chinese medicinal herb. In this study, using S. siamensis cultivated in vitro, twelve candidate reference genes under various treatments were analyzed for their expression stability by using algorithms such as GeNorm ...
Wenqiang Chen, Xiaodong Lin, Yan Wang, Detian Mu, C. Mo, Huaxue Huang, Huan Zhao, Zuliang Luo, Dai Liu, Iain W. Wilson, Deyou Qiu, Qi Tang   +11 more
semanticscholar   +1 more source

Selection and validation of reference genes desirable for gene expression analysis by qRT-PCR in MeJA-treated ginseng hairy roots.

PLoS ONE, 2019
Ginseng is a valuable herb of traditional Chinese medicine and ginsenosides, the main bioactive components of ginseng, have been proven to have multiple functions in human therapies and health.
Li Li, Kangyu Wang, Mingzhu Zhao, Shaokun Li, Yue Jiang, Lei Zhu, Jing Chen, Yanfang Wang, Chunyu Sun, Ping Chen, Jun Lei, Meiping Zhang, Yi Wang   +12 more
doaj   +1 more source

Selection and Validation of Reference Genes for RT-qPCR Analysis of the Ladybird Beetle Henosepilachna vigintioctomaculata

Frontiers in Physiology, 2018
Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a momentous technique for quantifying expression levels of the targeted genes across various biological processes.
Jing Lü, Shimin Chen, Mujuan Guo, Cuiyi Ye, B. Qiu, Jianhui Wu, Chunxiao Yang, Huipeng Pan   +7 more
semanticscholar   +1 more source

Selection of reference genes for quantitative RT-PCR (RT-qPCR) analysis of rat tissues under physiological and toxicological conditions [PDF]

, 2015
In biological research the analysis of gene expression levels in cells and tissues can be a powerful tool to gain insights into biological processes. For this, quantitative RT-PCR (RT-qPCR) is a popular method that often involve the use of constitutively
Hadrup, Niels, Hass, Ulla, Letting, Heidi, Svingen, Terje, Vinggaard, Anne Marie   +4 more
core   +3 more sources