Results 111 to 120 of about 6,430 (223)

Stability ranking of 20 candidate reference genes using geNorm,Normfinder and Bestkeeper.

, 2014
Notes: LP, leaf-expansion period; FS, the flower stage; GFS, the green fruit stage; RFS, the red fruit stages; RGS, the root growing after fruit stage. G,geNorm software; N, Normfinder software; B, Bestkeeper software.
Yu Zhao (104852), Minying Sun (660055), Linlin Zhu (514231), Qun Wang (102815), Michael Yang (660056), Jing Liu (38537)   +5 more
core   +1 more source

Differences in the suitability of published honey bee (Apis mellifera) reference genes between the African subspecies Apis mellifera scutellata and European derived Apis mellifera

Molecular Ecology, Volume 34, Issue 15, August 2025.
Abstract Quantitative real‐time polymerase chain reaction (qPCR) is a method widely used to determine changes and differences in gene expression. As target gene expression is most often quantified relative to the expression of reference genes, the validation of suitable reference genes is of critical importance.
Anja Buttstedt, Christian W. W. Pirk, Abdullahi A. Yusuf   +2 more
wiley   +1 more source

Descriptive statistics and expression level obtained from BestKeeper.

, 2016
Descriptive statistics and expression level obtained from BestKeeper.
Tian Deng (2635531), Letian Chen (2635528), Shouzhou Zhang (2635534), Yanting Hu (759128), Hong Wu (105254)   +4 more
core   +1 more source

Identification of Suitable Reference Genes for Gene Expression Normalization in Jatropha curcas L During Development and Under Stress Conditions Using Real Time Quantitative PCR

Brazilian Archives of Biology and Technology
Jatropha curcas L represent a potential source of raw material for the production of biodiesel. The aim this study was to find potential candidate reference genes in J. curcas tissues.
Antonio José Rocha, Paulo Abraão Maranhão, Rafaela Oliveira Silva, Simone Pohl, Cristiane S.R Fonteles   +4 more
doaj   +1 more source

Highly Targeted Metabolomics Coupled With Gene Expression Analysis by RT–qPCR Improves Beef Separation Based on Grass, Grain, or Grape Supplemented Diet

Food Frontiers, Volume 6, Issue 3, Page 1483-1497, May 2025.
ABSTRACT The objective of this study was to use a multi‐omics (i.e., gene expression quantification, metabolomics, and fatty acid [FA] profiling) approach to separate and authenticate beef from three different dietary groups. In this 2‐year study, Red Angus steers (n = 54) were randomly allocated to one of three treatments: (1) complex biodiverse ...
Lucas Krusinski, Chloe Castanon, Rosalee S. Hellberg, Isabella C. F. Maciel, Muhammad Ahsin, Stephan van Vliet, Jason E. Rowntree, Jenifer I. Fenton   +7 more
wiley   +1 more source

Stability analysis of 17 candidate reference genes using BestKeeper.

, 2018
Stability analysis of 17 candidate reference genes using BestKeeper.
Hyun Park (106467), Mira Park (212785), Hyoungseok Lee (142509), Soon Gyu Hong (409166), Byeong-ha Lee (650490)   +4 more
core   +1 more source

Pathogen and Gene Expression Profiles of Atlantic Salmon From an Endangered Population

Aquatic Conservation: Marine and Freshwater Ecosystems, Volume 35, Issue 4, April 2025.
ABSTRACT The role of pathogens in impacting the behaviour and fate of salmonids has been studied extensively for some selected pathogens such as sea lice. However, the whole pathobiome of the fish are seldom considered and may confound and influence the study species in situ.
Robert J. Lennox, Angela D. Schulze, Kristi M. Miller, Trond Einar Isaksen, Eirik Straume Normann, Cecilie Iden Nilsen, Lotte S. Dahlmo, Bjørn T. Barlaup, Elisabeth Stöger, Sindre H. Eldøy, Knut Wiik Vollset   +10 more
wiley   +1 more source

NormiRazor: tool applying GPU-accelerated computing for determination of internal references in microRNA transcription studies

BMC Bioinformatics, 2020
Background Multi-gene expression assays are an attractive tool in revealing complex regulatory mechanisms in living organisms. Normalization is an indispensable step of data analysis in all those studies, since it removes unwanted, non-biological ...
Szymon Grabia, Urszula Smyczynska, Konrad Pagacz, Wojciech Fendler   +3 more
doaj   +1 more source

Selection and Validation of Reference Genes for RT-qPCR Analysis of the Ladybird Beetle Henosepilachna vigintioctomaculata

Frontiers in Physiology, 2018
Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a momentous technique for quantifying expression levels of the targeted genes across various biological processes.
Jing Lu, Shimin Chen, Mujuan Guo, Cuiyi Ye, Bao-Li Qiu, Jian-Hui Wu, Chunxiao Yang, Huipeng Pan   +7 more
semanticscholar   +1 more source

Repeated pair-wise correlation amongst genes with BestKeeper index.

, 2018
Repeated pair-wise correlation amongst genes with BestKeeper index.
Ramneek Kaur (465837), Vijay Lakshmi Sharma (4915405), Manishi Mukesh (612982), Shelesh Kumar Swami (4915402), Monika Sodhi (3183042), Preeti Verma (4915408), Ankita Sharma (3183054), Parvesh Kumari (4915411)   +7 more
core   +1 more source