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Selection and Validation of Stable Reference Genes for RT-qPCR Analyses of <i>Rumex patientia</i> (Polygonaceae) Under Four Abiotic Stresses. [PDF]

open access: yesGenes (Basel)
Yang Q   +11 more
europepmc   +1 more source
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Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper – Excel-based tool using pair-wise correlations

Biotechnology Letters, 2004
The stability of standard gene expression is an elementary prerequisite for internal standardisation of target gene expression data and many so called housekeeping genes with assumed stable expression can exhibit either up- or down-regulation under some experimental conditions.
Michael W, Pfaffl   +3 more
openaire   +2 more sources

Stability of endogenous reference genes in postmortem human brains for normalization of quantitative real-time PCR data: comprehensive evaluation using geNorm, NormFinder, and BestKeeper

International Journal of Legal Medicine, 2012
In forensic molecular pathology, quantitative real-time polymerase chain reaction (RT-qPCR) provides a rapid and sensitive method to investigate functional changes in the death process. Accurate and reliable relative RT-qPCR requires ideal amplification efficiencies of target and reference genes.
Qi, Wang   +5 more
openaire   +2 more sources

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