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Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper – Excel-based tool using pair-wise correlations

Biotechnology Letters, 2004
The stability of standard gene expression is an elementary prerequisite for internal standardisation of target gene expression data and many so called housekeeping genes with assumed stable expression can exhibit either up- or down-regulation under some experimental conditions.
Michael W Pfaffl, Ales Tichopad
exaly   +4 more sources

Stability of endogenous reference genes in postmortem human brains for normalization of quantitative real-time PCR data: comprehensive evaluation using geNorm, NormFinder, and BestKeeper

International Journal of Legal Medicine, 2012
In forensic molecular pathology, quantitative real-time polymerase chain reaction (RT-qPCR) provides a rapid and sensitive method to investigate functional changes in the death process. Accurate and reliable relative RT-qPCR requires ideal amplification efficiencies of target and reference genes.
Qi Wang, Takaki Ishikawa, Bao-Li Zhu
exaly   +4 more sources

Evaluation of reference genes for RT-qPCR gene expression analysis in Arabidopsis thaliana exposed to elevated temperatures.

Plant biology, 2022
Increases in environmental temperature are directly linked to the issue of climate change and are known to significantly disrupt plant growth and development. Studies of gene expression in plants commonly include RT-qPCR but the reliability of the method
A. Škiljaica   +5 more
semanticscholar   +1 more source

RefFinder: a web-based tool for comprehensively analyzing and identifying reference genes

Functional & Integrative Genomics, 2023
Fuliang Xie, Jianyu Wang, Bao-Hong Zhang
semanticscholar   +1 more source

TBP, PPIA, YWHAZ and EF1A1 Are the Most Stably Expressed Genes during Osteogenic Differentiation

International Journal of Molecular Sciences, 2022
Janja Marc   +2 more
exaly  

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