Results 161 to 170 of about 1,513,732 (317)

An efficient strategy for producing RNA‐free Nucleocapsid protein of SARS‐CoV‐2 for biochemical and structural investigations

open access: yesFEBS Open Bio, EarlyView.
Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley   +1 more source

Interaction of class III cellobiose dehydrogenase with lytic polysaccharide monooxygenase

open access: yesFEBS Open Bio, EarlyView.
The activity of lytic polysaccharide monooxygenase (LPMO) is supported by its auxiliary enzyme cellobiose dehydrogenase (CDH). The catalytic activity of both enzymes is coupled by electron transfer and a cyclic cascade generating substrates for both enzymes – hydrogen peroxide for LPMO and oxidized and non‐oxidized cellobiose and cello‐oligosaccharide ...
Angela Giorgianni   +4 more
wiley   +1 more source

Mutations in the proximal binding site and F-loop of AdeJ confer resistance to efflux pump inhibitors. [PDF]

open access: yesAntimicrob Agents Chemother
Saral Sariyer A   +10 more
europepmc   +1 more source

Characterization of the thermophilic xylanase Fsa02490Xyn from the hyperthermophile Fervidibacter sacchari belonging to glycoside hydrolase family 10

open access: yesFEBS Open Bio, EarlyView.
Fervidibacter sacchari is a member of the bacterial phylum Armatimonadota, only a few of which have been cultivated. It is a hyperthermophilic polysaccharide degradation specialist. We show that a secreted enzyme, Fsa02490Xyn, is an endoxylanase belonging to glycoside hydrolase family 10.
Nicole Torosian   +10 more
wiley   +1 more source

Correction: Iceland screens, treats, or prevents multiple myeloma (iStopMM): a population-based screening study for monoclonal gammopathy of undetermined significance and randomized controlled trial of follow-up strategies

open access: yesBlood Cancer Journal, 2023
Sæmundur Rögnvaldsson   +34 more
doaj   +1 more source

Characterization of ribosome heterogeneity during endothelial to hematopoietic transition

open access: yesFEBS Open Bio, EarlyView.
The panorama of ribosome heterogeneity during embryonic hematopoiesis has not yet been portrayed. In this study, utilizing dual‐omics data, the heterogenous dynamic of ribosome during endothelial‐to‐hematopoietic transition has been systemically described. Moreover, stage‐specific upregulation and peripheral localization of RPL27 and RACK1 in hemogenic
Xitong Tian   +4 more
wiley   +1 more source

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