An efficient targeted nuclease strategy for high-resolution mapping of DNA binding sites
bioRxiv, 2016 We describe Cleavage Under Targets and Release Using Nuclease (CUT&RUN), a chromatin profiling strategy in which antibody-targeted controlled cleavage by micrococcal nuclease releases specific protein-DNA complexes into the supernatant for paired-end DNA
P. Skene, S. Henikoff
semanticscholar +1 more source
De novo prediction of PTBP1 binding and splicing targets reveals unexpected features of its RNA recognition and function. [PDF]
, 2014 The splicing regulator Polypyrimidine Tract Binding Protein (PTBP1) has four RNA binding domains that each binds a short pyrimidine element, allowing recognition of diverse pyrimidine-rich sequences.
Black, Douglas L +5 more
core +4 more sources
Identification of transcription factor binding sites using ATAC-seq
Genome Biology, 2018 Transposase-Accessible Chromatin followed by sequencing (ATAC-seq) is a simple protocol for detection of open chromatin. Computational footprinting, the search for regions with depletion of cleavage events due to transcription factor binding, is poorly ...
Zhijian Li +5 more
semanticscholar +1 more source
Diversity of molecular targets and signaling pathways for CBD
Pharmacology Research & Perspectives, 2020 Cannabidiol (CBD) is the second most abundant component of the Cannabis plant and is known to have effects distinct from Δ9‐tetrahydrocannabinol (THC). Many studies that examined the behavioral effects of CBD concluded that it lacks the psychotomimetic ...
Douglas L. deAlmeida, Lakshmi A. Devi
doaj +1 more source
Nucleic Acids Res., 2018 The identification of protein–ligand binding sites is critical to protein function annotation and drug discovery. The consensus algorithm COACH developed by us represents one of the most efficient approaches to protein–ligand binding sites prediction ...
Qi Wu +3 more
semanticscholar +1 more source
Journal of Lipid Research, 1972 Quantitative aspects of the binding of free fatty acid to human erythrocytes were studied by measuring the distribution of various amounts of [1-14C]lauric acid between washed human erythrocytes and defatted human plasma albumin. Incubations were done at
Arthur A. Spector +3 more
doaj +1 more source
A novel prediction method for protein DNA-binding residues based on neighboring residue correlations
Biotechnology & Biotechnological Equipment, 2022 Accurately identifying the protein DNA-binding residues is important for understanding the protein–DNA recognition mechanism and protein function annotation.
Jiazhi Song, Guixia Liu, Jingqing Jiang
doaj +1 more source
Recurrent Neural Network for Predicting Transcription Factor Binding Sites
Scientific Reports, 2018 It is well known that DNA sequence contains a certain amount of transcription factors (TF) binding sites, and only part of them are identified through biological experiments. However, these experiments are expensive and time-consuming.
Zhen Shen, Wenzheng Bao, De-shuang Huang
semanticscholar +1 more source
Amyloid and Tau Positron Emission Tomography Imaging in Alzheimer’s Disease and Other Tauopathies
Frontiers in Aging Neuroscience, 2022 The detection and staging of Alzheimer’s disease (AD) using non-invasive imaging biomarkers is of substantial clinical importance. Positron emission tomography (PET) provides readouts to uncover molecular alterations in the brains of AD patients with ...
Cinzia Maschio, Ruiqing Ni, Ruiqing Ni
doaj +1 more source
cAMP activates adenylate and guanylate cyclase of Dictyostelium discoideum cells by binding to different classes of cell-surface receptors. A study with extracellular Ca2+ [PDF]
, 1985 cAMP induces a transient increase of cAMP and cGMP levels in Dictyostelium discoideum cells. Fast binding experiments reveal three types of cAMP-binding site (S, H and L), which have different off-rates (t0.5, 0.7-15 s) and different affinities (Kd, 15 ...
Brachet +25 more
core +3 more sources