Results 11 to 20 of about 1,093 (175)

Low-Input Assay for Transposase-Accessible Chromatin Identifies Epigenetic Signatures of Liver Group 1 Innate Lymphoid Cells. [PDF]

Eur J Immunol
Assessing chromatin accessibility in rare cell populations within tissue remains a key challenge. To address this, we present a low‐input ATAC workflow optimized for liver ILCs. The protocol is validated across cell numbers, Tn5 ratios, and library preparation steps and unveils unique epigenetic features of liver NK cells and ILC1s.
Schmid K, Schenk RP, Wiedemann GM.
europepmc   +2 more sources

Scaling relation between genome length and particle size of viruses provides insights into viral life history

iScience, 2021
Summary: In terms of genome and particle sizes, viruses exhibit great diversity. With the discovery of several nucleocytoplasmic large DNA viruses (NCLDVs) and jumbo phages, the relationship between particle and genome sizes has emerged as an important ...
Harshali V. Chaudhari, Mandar M. Inamdar, Kiran Kondabagil   +2 more
doaj   +1 more source

2D effects enhance precision of gradient-based tissue patterning

iScience, 2023
Summary: Robust embryonic development requires pattern formation with high spatial accuracy. In epithelial tissues that are patterned by morphogen gradients, the emerging patterns achieve levels of precision that have recently been explained by a simple ...
Yuchong Long, Roman Vetter, Dagmar Iber
doaj   +1 more source

The computational rules of cross-modality suppression in the visual posterior sylvian area

iScience, 2023
Summary: The macaque visual posterior sylvian area (VPS) is an area with neurons responding selectively to heading direction in both visual and vestibular modalities, but how VPS neurons combined these two sensory signals is still unknown. In contrast to
Bin Zhao, Rong Wang, Zhihua Zhu, Qianli Yang, Aihua Chen   +4 more
doaj   +1 more source

Computational characterization of recombinase circuits for periodic behaviors

iScience, 2023
Summary: Recombinases are site-specific proteins found in nature that are capable of rearranging DNA. This function has made them promising gene editing tools in synthetic biology, as well as key elements in complex artificial gene circuits implementing ...
Judith Landau, Christian Cuba Samaniego, Giulia Giordano, Elisa Franco   +3 more
doaj   +1 more source

Predicting Human Clinical Outcomes Using Mouse Multi-Organ Transcriptome

iScience, 2020
Summary: Approximately 90% of pre-clinically validated drugs fail in clinical trials owing to unanticipated clinical outcomes, costing over several hundred million US dollars per drug.
Satoshi Kozawa, Fumihiko Sagawa, Satsuki Endo, Glicia Maria De Almeida, Yuto Mitsuishi, Thomas N. Sato   +5 more
doaj   +1 more source

In silico assessment of a novel single-molecule protein fingerprinting method employing fragmentation and nanopore detection

iScience, 2021
Summary: The identification of proteins at the single-molecule level would open exciting new venues in biological research and disease diagnostics.
Carlos de Lannoy, Florian Leonardus Rudolfus Lucas, Giovanni Maglia, Dick de Ridder   +3 more
doaj   +1 more source

A geometric framework for understanding dynamic information integration in context-dependent computation

iScience, 2021
Summary: The prefrontal cortex (PFC) plays a prominent role in performing flexible cognitive functions and working memory, yet the underlying computational principle remains poorly understood.
Xiaohan Zhang, Shenquan Liu, Zhe Sage Chen   +2 more
doaj   +1 more source

Comprehensive multi-omics single-cell data integration reveals greater heterogeneity in the human immune system

iScience, 2022
Summary: Single-cell transcriptomics enables the definition of diverse human immune cell types across multiple tissues and disease contexts. Further deeper biological understanding requires comprehensive integration of multiple single-cell omics ...
Congmin Xu, Junkai Yang, Astrid Kosters, Benjamin R. Babcock, Peng Qiu, Eliver E.B. Ghosn   +5 more
doaj   +1 more source

Identifying Transcription Factor Combinations to Modulate Circadian Rhythms by Leveraging Virtual Knockouts on Transcription Networks

iScience, 2020
Summary: The mammalian circadian systems consist of indigenous, self-sustained 24-h rhythm generators. They comprise many genes, molecules, and regulators. To decode their systematic controls, a robust computational approach was employed.
Debajyoti Chowdhury, Chao Wang, Aiping Lu, Hailong Zhu   +3 more
doaj   +1 more source