Results 91 to 100 of about 11,629 (239)

Live-Cell Bioorthogonal Chemical Imaging: Stimulated Raman Scattering Microscopy of Vibrational Probes [PDF]

, 2016
Innovations in light microscopy have tremendously revolutionized the way researchers study biological systems with subcellular resolution. In particular, fluorescence microscopy with the expanding choices of fluorescent probes has provided a ...
Chen, Zhixing, Hu, Fanghao, Min, Wei, Shen, Yihui, Wei, Lu, Zhang, Luyuan   +5 more
core  

Molecular basis for functional switching of GFP by two disparate non-native post-translational modifications of a phenyl azide reaction handle [PDF]

, 2016
Through the genetic incorporation of a single phenyl azide group into superfolder GFP (sfGFP) at residue 148 we provide a molecular description of how this highly versatile chemical handle can be used to positively switch protein function in vitro and in
Hartley, Andrew M., Jones, D. Dafydd, Reddington, Samuel C., Rizkallah, Pierre J., Worthy, Harley L.   +4 more
core   +3 more sources

Reactive polymer enables efficient in vivo bioorthogonal chemistry [PDF]

Proceedings of the National Academy of Sciences, 2012
There has been intense interest in the development of selective bioorthogonal reactions or “click” chemistry that can proceed in live animals. Until now however, most reactions still require vast surpluses of reactants because of steep temporal and spatial concentration gradients.
Neal K, Devaraj, Greg M, Thurber, Edmund J, Keliher, Brett, Marinelli, Ralph, Weissleder   +4 more
openaire   +2 more sources

Bioorthogonal “Click” Cycloadditions: A Toolkit for Modulating Polymers and Nanostructures in Living Systems

Reactions
“Click” cycloadditions offer effective pathways for the modifications of supramolecular structures, polymers, and nanomaterials. These reactions include bioorthogonal mechanisms that do not interfere with the biological processes, providing a type of ...
Irene Lepori, Yavuz Oz, Jungkyun Im, Nandan Ghosh, Mohuya Paul, Ulrich S. Schubert, Stefano Fedeli   +6 more
doaj   +1 more source

Direct Intracellular Delivery of Cell Impermeable Probes of Protein Glycosylation Using Nanostraws [PDF]

, 2017
Bioorthogonal chemistry is an effective tool for elucidating metabolic pathways and measuring cellular activity, yet its use is currently limited by the difficulty of getting probes past the cell membrane and into the cytoplasm, especially if more ...
Cao, Yuhong, Melosh, Nicholas A., Shieh, Peyton, Wang, Derek S., Xu, Alexander M.   +4 more
core  

Tailoring Chimeric Ligands for Studying and Biasing ErbB Receptor Family Interactions [PDF]

, 2013
Described is the development and application of a versatile semisynthetic strategy, based on a combination of sortase-mediated coupling and tetrazine ligation chemistry, which can be exploited for the efficient incorporation of tunable functionality into
Amin, Baselga, Bundy, Butt, Chen, Ciardiello, Citri, Devaraj, Engelman, Hudak, Inagaki, Iyer, Jay, Jay, Jeppesen, Karver, Kumar, Lee, Lemmon, Linggi, Lu, Mammen, Marblestone, Martino, Nagel, Nandagopal, Olayioye, Popp, Proft, Reddy, Riese, Rodrigues, Sainlos, Scheck, Schellinger, Tanaka, Wiley, Wingens, Witte, Wu   +39 more
core   +1 more source

Photocaged Oxytocin and Vasopressin Probes to Decipher Neuropeptide Signalling With High Spatiotemporal Resolution

Angewandte Chemie International Edition, EarlyView.
Novel and biocompatible photoactivatable oxytocin and vasopressin probes, utilising state‐of‐the‐art photocages, remain inactive in the dark. Light exposure facilitates rapid spatiotemporal receptor activation and phospholipase C (PLC)‐mediated downstream signalling. Figure created in https://BioRender.com.
Konstantin Raabe, Predrag Kalaba, Xuan Ling Hilary Yong, Greta Crudeli, Sarah Melzer, Erik Keimpema, Victor Anggono, Markus Muttenthaler   +7 more
wiley   +1 more source

Ring Expansion Of Alkylidenecarbenes Derived From Lactams, Lactones, And Thiolactones Into Strained Heterocyclic Alkynes: A Theoretical Study [PDF]

, 2019
Strained cycloalkynes are of considerable interest to theoreticians and experimentalists, and possess much synthetic value as well. Herein, a series of cyclic alkylidenecarbenes—formally obtained by replacing the carbonyl oxygen of four-, five-, and six ...
Just, J., Pankauski, J. M., Rablen, Paul R., Thamattoor, D. M., Thu Le, N. N.   +4 more
core   +1 more source

A Synergistic Inhibitor Development Strategy Against Human UDP‐Galactose‐4‐Epimerase

Angewandte Chemie International Edition, EarlyView.
The epimerase GalE is crucial for the biosynthesis of cancer‐relevant O‐GalNAc glycans. Here, we employ orthogonal, structurally enabled small molecule fragment screens to yield both covalent and non‐covalent inhibitors against GalE within no more than 22 elaborated compounds.
William M. Browne, Jonathan Pettinger, Teresa Weckwerth, Andrew Purkiss, Sing Hei Lok, Louisa Penicaut, Roksana Ogrodowicz, Raveena Prema, Simone Kunzelmann, Chloe Roustan, Ganka Bineva‐Todd, Saskia Pieters, Francesca Zappacosta, Alfred E. Doherty, Isobel Oram, Christelle Soudy, Robert Quinlan, Joanna Redmond, Svend Kjaer, David House, Stephane Mouilleron, Jacob T. Bush, Benjamin Schumann   +22 more
wiley   +1 more source

T Cell Membrane Mimicking Nanoparticles with Bioorthogonal Targeting and Immune Recognition for Enhanced Photothermal Therapy

Advanced Science, 2019
Due to specific immune recognition receptors on the surface of T cells, their membranes are promising mimic nanocarriers for delivering drugs to tumor lesions.
Yutong Han, Hong Pan, Wenjun Li, Ze Chen, Aiqing Ma, Ting Yin, Ruijing Liang, Fuming Chen, Yifan Ma, Yan Jin, Mingbin Zheng, Baohong Li, Lintao Cai   +12 more
doaj   +1 more source