Results 81 to 90 of about 2,778,442 (233)

Bioorthogonal chemistry in living organisms

Chemical Science, 2014
Bioorthogonal chemistry allows for selective and efficient modification of biomolecules in their natural environment. Several strategies have been developed over the past years that employ cellular biosynthetic pathways to incorporate the desired functionalities.
Borrmann, A., van Hest, J.C.M., van Hest, J.C.M.   +2 more
openaire   +2 more sources

Enzyme‐Linked Cycloaddition Assay (ELCA) for Rapid, Ultra‐Sensitive Monitoring of Secreted Sialoglycoproteins

Advanced Science, EarlyView.
Secreted glycoproteins report on cellular state faster than the cell surface, yet remain hard to measure. The Enzyme‐Linked Cycloaddition Assay (ELCA) combines metabolic azide labeling with click chemistry in an ELISA‐like microplate format, resolving sialylation changes within minutes for 1‐2 EUR per sample.
Jon Lundstrøm, Jingmei Yang, Daniel Bojar   +2 more
wiley   +1 more source

A Fluorogenic Cysteine‐Tetrapeptide Tagging Strategy for Nonperturbative Protein Labeling and Protein–Ligand Interaction Tracking

Advanced Science, EarlyView.
A compact Phe‐Cys‐Pro‐Phe (FCPF) tag and an environment‐sensitive SBD‐Cl‐derived probe form a sequence‐selective fluorogenic pair for wash‐free live‐cell protein imaging. This minimally perturbing chemistry allows flexible insertion at termini or loops, produces strong fluorescence upon local microenvironment confinement, supports FRET‐assisted ...
Xiao Wang, Xingcan Pan, Yongling Ying, Ziqian Bai, Jingming Zhang, Zhuang Shao, Jialiang Qian, Hongwei Li, Ning Zhang, Fan Yang, Yuan Liu, Xing Yang   +11 more
wiley   +1 more source

Carbohydrate‐Directed Labeling Probes for UV‐Independent Chemoproteomic Profiling of Carbohydrate–Protein Interactions

Angewandte Chemie International Edition, Volume 65, Issue 44, 26 October 2026.
Modular carbohydrate‐directed labeling probes (CDLPs) convert weak carbohydrate–protein interactions into durable covalent tags in live cells without the need for multivalency or UV irradiation. Ligand binding triggers proximity‐enabled, glycan‐releasing acyl transfer to install a click handle for protein tagging and chemoproteomic profiling.
Matthew C. Russolillo, Wariya Nirachonkul, Nora Ross, Rahul M. Hedau, Zachary Clark, Michaella J. Rekowski, Michael P. Washburn, Mark P. Farrell   +7 more
wiley   +2 more sources

Exploring New Bioorthogonal Catalysts: Scaffold Diversity in Catalysis for Chemical Biology

Advanced Science
Bioorthogonal catalysis has revolutionized the field of chemical biology by enabling selective and controlled chemical transformations within living systems.
Yan Zhang, Qizhen Huang, Fang Lei, Wanlong Qian, Chengfeng Zhang, Qi Wang, Chaoqun Liu, Haiwei Ji, Faming Wang   +8 more
doaj   +1 more source

Cancer's sweet spot: techniques to harness saccharides in tumor biology

BioTechniques
All of the cells in our bodies are enveloped in sugar, this sweet coating plays a particularly interesting and crucial role in tumor biology. Here, we review the techniques being used to detect and exploit cancer's sweet spot.
Jade Parker
doaj   +1 more source

Discovery and Application of a Lysine 5-Hydroxylase for Bioorthogonal Chemistry [PDF]

The selective functionalization of unactivated C(sp3)–H bonds remains an ongoing challenge in synthetic organic chemistry. In this context, biocatalysis provides an attractive strategy to perform such chemistry under mild reaction conditions.
Elizabeth, Stone, Nicole, Angelisanti, Adriana, Vargas-Figueroa, Andrew, Whitten, Douglas, Millar, Michelle, Chang, Elijah, Kissman   +6 more
core   +2 more sources

Aktivierung von Metallkomplexen durch Ultraschallwellen für eine gezielte Krebstherapie

Angewandte Chemie, EarlyView.
Ultrasound enables non‐invasive activation of metal‐based therapeutics deep within tumors. Acoustic cavitation can drive sonodynamic reactive oxygen species generation or mechanochemical metal–ligand bond dissociation, enabling controlled release of bioactive payloads.
Félix Grosjean, Nicolás Montesdeoca, Gilles Berger, Johannes Karges   +3 more
wiley   +1 more source

Rethinking Click and Bioorthogonal Chemistry for Biomedical Applications

, 2023
International audienceOver the last two decades, click chemistry and bioorthogonal chemistry have revolutionized the field of biomedical engineering.
Delplace, Vianney
core   +1 more source

Bioorthogonal Swarming: In Situ Generation of Dendrimers [PDF]

, 2020
With the aid of bioorthogonal chemistry, we demonstrate the fabrication of synthetic dendrimers in situ around living cells. Using tetrazine dienophile and aminooxyl/hydrazide aldehyde chemistries, the density of functional groups on the dendrimers ...
Baibek, Assel, Zhang, Shuo, Ucuncu, M, Üçüncü, Muhammed, Zhang, S, Lilienkampf, A, Lilienkampf, Annamaria; id_orcid, Zhang, Y, Gambardella, Alessia, Geng, J, Baibek, A, Bradley, M, Lilienkampf, Annamaria, Bradley, Mark, Litzen, Inga, Zhang, Yichuan, Gambardella, A, Clavadetscher, J, Geng, Jin, Litzen, I, Clavadetscher, Jessica   +20 more
core   +1 more source