Results 31 to 40 of about 2,778,442 (233)
Affinity Bioorthogonal Chemistry (ABC) Tags for Site-Selective Conjugation, On-Resin Protein-Protein Coupling, and Purification of Protein Conjugates. [PDF]
Angew Chem Int Ed Engl, 2022 The site-selective functionalization of proteins has broad application in chemical biology, but can be limited when mixtures result from incomplete conversion or the formation of protein containing side products.
Scinto SL, Reagle TR, Fox JM.
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Bioorthogonal Reactions in Activity-Based Protein Profiling
Molecules, 2020 Activity-based protein profiling (ABPP) is a powerful technique to label and detect active enzyme species within cell lysates, cells, or whole animals. In the last two decades, a wide variety of applications and experimental read-out techniques have been
Steven H. L. Verhelst +2 more
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A Decade of Bioorthogonal Chemistry [PDF]
Accounts of Chemical Research, 2011 Inventing new chemical reactions is a long-standing passion of chemists, as reflected in the ever-popular field of reaction methodology. Historically, the environment in which newly crafted transformations would be performed imposed minimal limitations on reaction design; after all, solvents can be screened to find an optimal choice, temperature can be
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Bioorthogonal Chemistry in Biomolecule Quantification: A Review of Reactions and Strategies. [PDF]
ChemistryABSTRACT Bioorthogonal chemistry has emerged as a transformative strategy for detecting and quantifying biomolecules in complex biological systems. This review highlights recent advances in catalyst‐free bioorthogonal reactions specifically applied to semi‐quantitative and quantitative biomolecular analysis.
Yang M, Wang S.
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Bioorthogonal chemistry: strategies and recent developments [PDF]
Chemical Communications, 2013 The use of covalent chemistry to track biomolecules in their native environment-a focus of bioorthogonal chemistry-has received considerable interest recently among chemical biologists and organic chemists alike. To facilitate wider adoption of bioorthogonal chemistry in biomedical research, a central effort in the last few years has been focused on ...
Carlo P, Ramil, Qing, Lin
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Metabolic glycoengineering – exploring glycosylation with bioorthogonal chemistry
Chemical Society Reviews, 2023 Metabolic glycoengineering in combination with bioorthogonal chemistry provides a means to study and exploit the biological functions of glycans.
Markus Kufleitner +2 more
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Minimal genetically encoded tags for fluorescent protein labeling in living neurons
Nature Communications, 2022 Site-specific labelling of proteins can be performed with unnatural amino acids combined with bioorthogonal click chemistry. Here the authors establish this in living neurons, using neurofilament light chain; they show combination with CRISPR/Cas9 ...
Aleksandra Arsić +4 more
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A Genetically Encoded Picolyl Azide for Improved Live Cell Copper Click Labeling
Frontiers in Chemistry, 2021 Bioorthogonal chemistry allows rapid and highly selective reactivity in biological environments. The copper-catalyzed azide–alkyne cycloaddition (CuAAC) is a classic bioorthogonal reaction routinely used to modify azides or alkynes that have been ...
Birthe Meineke +9 more
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STAR Protocols, 2023 Summary: Bioorthogonal labeling and click chemistry techniques allow the detailed examination of cellular physiology through tagging and visualizing newly synthesized proteins.
Alison Keolani Carlisle +2 more
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Bioorthogonal Photocatalytic Profiling of Mitochondrial Proteomes from Primary Living Samples [PDF]
, 2023 In situ profiling of subcellular proteomic networks in primary and living systems, such as primary cells derived from native tissues or clinic samples, is crucial for the understanding of life processes and diseases, yet challenging for the current ...
Xinyuan, Fan +8 more
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