Results 61 to 70 of about 5,912 (181)

Orthogonal Light‐ and Enzyme‐Triggered and‐Gate Prodrugs for Senolytic Therapy

Advanced Science, EarlyView.
An orthogonal AND‐gate prodrug platform combines 390 nm near‐UV photocleavage with SA‐β‐gal activation to control payload release in senescent cells. Its modular architecture enables 4‐MU fluorescence, doxorubicin‐mediated cytotoxicity, and ARV‐771‐driven BRD4 degradation, supporting stimulus‐controlled senolytic design in cellular and organoid models.
Mengyang Chang, Kaixue Zhang, Junwei Wang, Fangchao Bi, Zhuoxin Dai, Miao Yang, Ming Liu, Huanzhi Peng, Franklin Zhang, Yuan Guo, Jigang Dai, Quanxing Liu, Wei Wang   +12 more
wiley   +1 more source

Carbohydrate‐Directed Labeling Probes for UV‐Independent Chemoproteomic Profiling of Carbohydrate–Protein Interactions

Angewandte Chemie International Edition, Volume 65, Issue 44, 26 October 2026.
Modular carbohydrate‐directed labeling probes (CDLPs) convert weak carbohydrate–protein interactions into durable covalent tags in live cells without the need for multivalency or UV irradiation. Ligand binding triggers proximity‐enabled, glycan‐releasing acyl transfer to install a click handle for protein tagging and chemoproteomic profiling.
Matthew C. Russolillo, Wariya Nirachonkul, Nora Ross, Rahul M. Hedau, Zachary Clark, Michaella J. Rekowski, Michael P. Washburn, Mark P. Farrell   +7 more
wiley   +2 more sources

A Synergistic Nanotherapeutic Platform Leveraging Bioorthogonal Amplification and Ultrasound Triggering for Multidrug‐Resistant Bacterial Clearance and Wound Repair

Aggregate
The rise of multidrug‐resistant (MDR) bacterial infections highlights the need for precise, non‐antibiotic therapeutic strategies. Herein, a bioorthogonal reaction‐mediated and ultrasound (US)‐responsive nanoplatform (denoted as MnCyNPs‐Tz) was developed
Wentao Lei, Binbin Fu, Cuizhou Luan, Jinhao Li, Sijia Huang, Jianping Shao, Zifei Wang, Shufen Ye, Zhengyin Pan, Liang He   +9 more
doaj   +1 more source

Enzyme‐Linked Cycloaddition Assay (ELCA) for Rapid, Ultra‐Sensitive Monitoring of Secreted Sialoglycoproteins

Advanced Science, EarlyView.
Secreted glycoproteins report on cellular state faster than the cell surface, yet remain hard to measure. The Enzyme‐Linked Cycloaddition Assay (ELCA) combines metabolic azide labeling with click chemistry in an ELISA‐like microplate format, resolving sialylation changes within minutes for 1‐2 EUR per sample.
Jon Lundstrøm, Jingmei Yang, Daniel Bojar   +2 more
wiley   +1 more source

Synthesis of non-linear protein dimers through a genetically encoded Thiol-ene reaction. [PDF]

PLoS ONE, 2014
Site-specific incorporation of bioorthogonal unnatural amino acids into proteins provides a useful tool for the installation of specific functionalities that will allow for the labeling of proteins with virtually any probe.
Jessica Torres-Kolbus, Chungjung Chou, Jihe Liu, Alexander Deiters   +3 more
doaj   +1 more source

A Fluorogenic Cysteine‐Tetrapeptide Tagging Strategy for Nonperturbative Protein Labeling and Protein–Ligand Interaction Tracking

Advanced Science, EarlyView.
A compact Phe‐Cys‐Pro‐Phe (FCPF) tag and an environment‐sensitive SBD‐Cl‐derived probe form a sequence‐selective fluorogenic pair for wash‐free live‐cell protein imaging. This minimally perturbing chemistry allows flexible insertion at termini or loops, produces strong fluorescence upon local microenvironment confinement, supports FRET‐assisted ...
Xiao Wang, Xingcan Pan, Yongling Ying, Ziqian Bai, Jingming Zhang, Zhuang Shao, Jialiang Qian, Hongwei Li, Ning Zhang, Fan Yang, Yuan Liu, Xing Yang   +11 more
wiley   +1 more source

Fluorogenic Coumarins Activated via Bioorthogonal Reaction

The Journal of Organic Chemistry
Fluorogenic bioorthogonal reagents enable facile detection in complex environments. While useful for real-time imaging, few such probes are available. Existing tools also exploit similar mechanisms for signal turn-on, precluding multiplexed applications. To address these gaps, we developed a palette of cyclopropenone (CpO) scaffolds that are activated
Halley M. Lin-Jones, Sharon Chen, Yeonseong Seo, Kelly Q. Pham, Jennifer A. Prescher   +4 more
openaire   +2 more sources

Preparation of Protein Lysates Using Biorthogonal Chemical Reporters for Click Reaction and in-Gel Fluorescence Analysis

Bio-Protocol
Bioorthogonal chemical reporters are non-native chemical handles introduced into biomolecules of living systems, typically through metabolic or protein engineering.
Yaxin Xu, Tao Peng
doaj   +1 more source

Fluorogenic and Bioorthogonal Modification of RNA Using Photoclick Chemistry

Biomolecules, 2020
A bromoaryltetrazole-modified uridine was synthesized as a new RNA building block for bioorthogonal, light-activated and postsynthetic modification with commercially available fluorescent dyes.
Katja Krell, Hans-Achim Wagenknecht
doaj   +1 more source

A Spatially Distributed Microneedle System for Bioorthogonal T Cell‐Guided Cancer Therapy

Advanced Science
Chimeric antigen receptor (CAR)‐T cell therapy represents a promising strategy for cancer treatment. However, the diversity of solid tumor antigens and the poor infiltration of CAR‐T cells significantly hinder the efficacy of CAR‐T therapies against ...
Lanya Li, Fei Wang, Shushan Mo, Junyao Deng, Xueyi Wang, Jiacong Ai, Yingxian Xiao, Yan Zeng, Qishan Li, Yixin Zhang, Limin Cai, Zhenhua Li   +11 more
doaj   +1 more source