Results 41 to 50 of about 10,672 (180)

Bioorthogonal Decaging Reactions for Targeted Drug Activation

CHIMIA, 2018
Bioorthogonal decaging reactions are highly selective transformations which involve the cleavage of a protecting group from a molecule of interest. Decaging reactions can be classified into subgroups depending on the nature of the trigger; they can be photo-, metal- or small molecule-triggered.
Davies, Sarah, Stenton, Benjamin J, Bernardes, Gonçalo JL   +2 more
openaire   +5 more sources

Cold Orthogonal Translation: A Psychrophilic Pyrrolysyl‐tRNA Synthetase Boosts Genetic Code Expansion in E. coli

Advanced Science, EarlyView.
ABSTRACT Orthogonal translation systems (OTSs) enable site‐specific incorporation of non‐canonical amino acids (ncAAs) and are central to genetic code expansion. Current engineering strategies typically rely on hyperstable aminoacyl tRNA synthetase (aaRS) scaffolds to tolerate destabilizing mutations required for substrate diversification.
Nikolaj G. Koch, Peter Goettig, Michael A. Nash, Juri Rappsilber, Nediljko Budisa   +4 more
wiley   +1 more source

Controlled Orientation of Active Sites in a Nanostructured Multienzyme Complex [PDF]

, 2016
Multistep cascade reactions in nature maximize reaction efficiency by co-assembling related enzymes. Such organization facilitates the processing of intermediates by downstream enzymes.
A Sachdeva, A Weckbecker, BM Hutchins, CC Roberts, CH Kim, CJ Delebecque, D Guan, DE Torres Pazmino, EM Brustad, H Choe, H Nanba, H Pettersson, HS Seo, J Fu, J Fu, J-L Lin, JA Johnson, JE Dueber, JW Chin, M Castellana, M Leibundgut, M Slatner, MG Hoesl, MH Seo, MJ Lajoie, ML Blackman, NJ Agard, O Idan, P Bauler, P Brunker, P Bubner, R Narayanaswamy, RJ Conrado, S An, S Schoffelen, SI Lim, SI Lim, SI Lim, SI Lim, SR Bond, TM Schmeing, TS Moon, Y Kim, Y Zhang   +43 more
core   +2 more sources

Development of Bioorthogonal Reactions and Their Applications in Bioconjugation [PDF]

Molecules, 2015
Biomolecule labeling using chemical probes with specific biological activities has played important roles for the elucidation of complicated biological processes. Selective bioconjugation strategies are highly-demanded in the construction of various small-molecule probes to explore complex biological systems.
Mengmeng Zheng, Li Zheng, Peiyuan Zhang, Jinbo Li, Yan Zhang   +4 more
openaire   +3 more sources

Sensing and Reprogramming Surface Receptor Activation With Synthetic Transcriptional Circuits

Advanced Science, EarlyView.
A synthetic receptor‐signalling induced transcription (RESIT) circuit is designed based on receptor activation mediated split protease complementation and release of membrane‐tethered synthetic transcriptional modules. The RESIT system enables probing Ca2+ entry, receptor tyrosine kinase (RTK) activities and Ras activation, and reprogramming RTK ...
Fei Liu, Mei Yuan, Li‐Juan Tang, Fenglin Wang, Xia Chu, Jian‐Hui Jiang   +5 more
wiley   +1 more source

Bioorthogonal Fluorogenic Reporters for Noninvasive Imaging and Urinalysis of Immunotherapeutic Response in Renal Cell Carcinoma

Advanced Science, EarlyView.
This study reported renal‐clearable bio‐orthogonal near‐infrared fluorogenic probes (BGRs) that specifically imaging and urinalysis of granzyme B for dynamic evaluation of RCC immunotherapy. BGRs not only differentiate immunotherapeutic responses in orthotopic RCC mice, but also enable sensitive optical urinalysis of granzyme B in clinical specimens ...
Xingyue Yang, Baoshuai Liang, Liangmin Fu, Weiliang Deng, Yuyan Jiang, Weiping Xu, Jinwei Chen, Ya Zhou, Ke Wu, Jiaguo Huang   +9 more
wiley   +1 more source

Engineering Oncolytic Virus‐Armed Macrophages for Enhanced Cancer Immunotherapy

Advanced Science, EarlyView.
ZIFOA‐M is engineered by conjugating oncolytic adenovirus‐loaded ZIF‐8 nanoparticles onto macrophage surfaces via bioorthogonal chemistry. Upon tumor infiltration, the platform releases OA to downregulate CD47/CD24 on tumor cells, restoring macrophage phagocytosis.
Jilong Wang, Ning Lu, Zhuo Yan, Luyi Ye, Luyao Bai, Shanshan Yuan, Yuting Zhu, Yiru Xiong, Yongheng Bai, Yaping Chen, Junjie Deng   +10 more
wiley   +1 more source

Bioorthogonal Modification of the Major Sheath Protein of Bacteriophage M13: Extending the Versatility of Bionanomaterial Scaffolds [PDF]

, 2016
This document is the unedited Author’s version of a Submitted Work that was subsequently accepted for publication in Biconjugate Chemistry, copyright © American Chemical Society after peer review.
Daub, Elisabeth, Honek, John, Urquhart, Taylor   +2 more
core   +2 more sources

Evaluation of Pyrones in Bioorthogonal Reactions: Correlation between Structure, Reactivity, and Bioorthogonality

The Journal of Organic Chemistry
Alpha-pyrones have been used for applications ranging from total synthesis to antibiotics. However, their application as dienes in bioorthogonal reactions has not been extensively explored. In previous work, we demonstrated the promising application of ester-functionalized pyrones in bioorthogonal protein labeling.
Wei Huang, Kangqiao Wen, Paul F. Muschitiello, Jorge Escorihuela, Scott T. Laughlin   +4 more
openaire   +3 more sources

Bionanomaterials from plant viruses [PDF]

, 2015
Plant virus capsids have emerged as useful biotemplates for material synthesis. All plant virus capsids are assembled with high-precision, three-dimensional structures providing nanoscale architectures that are highly monodisperse, can be produced in ...
Aljabali, Alaa A. A., Evans, David
core   +2 more sources