Orthogonal, metal-free surface modification by strain-promoted azide–alkyne and nitrile oxide–alkene/alkyne cycloadditions [PDF]
, 2012 In this article we present a fast and efficient methodology for biochemical surface patterning under extremely mild conditions. Micropatterned azide/benzaldoxime-surfaces were prepared by microcontact printing of a heterobifunctional cyclooctyne oxime ...
Arlinghaus, Heinrich F +6 more
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Site-selective protein-modification chemistry for basic biology and drug development. [PDF]
, 2015 Nature has produced intricate machinery to covalently diversify the structure of proteins after their synthesis in the ribosome. In an attempt to mimic nature, chemists have developed a large set of reactions that enable post-expression modification of ...
A Perols +78 more
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Isonitrile-responsive and bioorthogonally removable tetrazine protecting groups. [PDF]
, 2020 In vivo compatible reactions have a broad range of possible applications in chemical biology and the pharmaceutical sciences. Here we report tetrazines that can be removed by exposure to isonitriles under very mild conditions.
Eckvahl, Hannah J +7 more
core
Anomeric O-Functionalization of Carbohydrates for Chemical Conjugation to Vaccine Constructs. [PDF]
, 2018 Carbohydrates mediate a wide range of biological interactions, and understanding these processes benefits the development of new therapeutics. Isolating sufficient quantities of glycoconjugates from biological samples remains a significant challenge ...
Gervay-Hague, Jacquelyn +2 more
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Convenient analysis of protein modification by chemical blotting with fluorogenic “click” reagents [PDF]
, 2015 Direct visualization of bioorthogonal alkyne or azide handles using fluorogenic azide–alkyne cycloaddition conducted on the surface of a blot membrane.
Ball, Zachary T. +2 more
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Advances in chemical protein modification. [PDF]
, 2015 Chemical protein modification has emerged as an invaluable tool for the development of modified proteins. The complementary use of both genetic and chemical methods has provided a large toolbox that allows the preparation of almost unlimited protein ...
Bernardes, Gonçalo JL, Boutureira, Omar
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Multicyclic D‐Stereospecific Hydrolase Dimer With High Sustained Activity
Angewandte Chemie, EarlyView.Protein macrocyclization was applied to a thermally unstable D‐stereospecific hydrolase using in situ cyclization of proteins (INCYPRO). This site‐specific cross‐linking approach enhanced the resistance of the enzyme to heat and cosolvents.
Anissa Haim +10 more
wiley +2 more sources
Iridium(iii) complexes of 1,2,4-triazines as potential bioorthogonal reagents: metal coordination facilitates luminogenic reaction with strained cyclooctynes [PDF]
, 2019 In this paper we describe unprecedented Ir(III) complexes of 5-(2-pyridyl)-1,2,4-triazine and their reactivity towards the strained cyclooctyne BCN. The coordination of a 1,2,4-triazine ring to an iridium(III) ion drastically increases the speed of the ...
Deary, Michael +4 more
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Chemical Metabolomics: Chemical Biology Tools for Advanced Metabolism Investigations
Angewandte Chemie, EarlyView.The human metabolism has been investigated for several millennia. The metabolome is known for a high complexity due to a large number of different metabolites that are present at different concentrations. Metabolomics has been developed as a field to investigate the entire human metabolome and to elucidate disease development mechanisms.
Alejandro Torregrosa‐Chinillach +4 more
wiley +2 more sources
Dual modification of biomolecules [PDF]
, 2016 With the advent of novel bioorthogonal reactions and “click” chemistry, an increasing number of strategies for the single labelling of proteins and oligonucleotides have emerged.
Chudasama, V, Maruani, A, Richards, DA
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