Results 21 to 30 of about 5,912 (181)

Constructing New Bioorthogonal Reagents and Reactions [PDF]

Accounts of Chemical Research, 2018
Chemical tools are transforming our understanding of biomolecules and living systems. Included in this group are bioorthogonal reagents-functional groups that are inert to most biological species, but can be selectively ligated with complementary probes, even in live cells and whole organisms.
R. David Row, Jennifer A. Prescher
openaire   +4 more sources

In Situ Bioorthogonal Synthesis of PROTACs via Dual-Responsive Cleavage for Synergistic Photo-Immunotherapy. [PDF]

Adv Sci (Weinh)
A bioactive self‐delivering SNAP‐TAC platform responds to tumor‐associated cathepsin B and glutathione, enabling in situ bioorthogonal synthesis of a BRD4‐degrading proteolysis‐targeting chimera within the tumor microenvironment. The resulting BRD4 degradation and PD‐L1 downregulation synergize with photodynamic therapy‐induced immunogenic cell death ...
Jian S, Wu J, Xu F, Zuo Y, Shen H, Ji R, Wang L, Li N, Sun Y, Yu Y, Shi Y, Hu H, Xu F, Huang D, Hu X.   +14 more
europepmc   +2 more sources

Overview of Syntheses and Molecular-Design Strategies for Tetrazine-Based Fluorogenic Probes

Molecules, 2021
Various bioorthogonal chemistries have been used for fluorescent imaging owing to the advantageous reactions they employ. Recent advances in bioorthogonal chemistry have revolutionized labeling strategies for fluorescence imaging, with inverse electron ...
Sang-Kee Choi, Jonghoon Kim, Eunha Kim
doaj   +1 more source

Kinugasa Reactions in Water: From Green Chemistry to Bioorthogonal Labelling

Molecules, 2015
The Kinugasa reaction has become an efficient method for the direct synthesis of β-lactams from substituted nitrones and copper(I) acetylides. In recent years, the reaction scope has been expanded to include the use of water as the solvent, and with ...
Mariya Chigrinova, Douglas A. MacKenzie, Allison R. Sherratt, Lawrence L. W. Cheung, John Paul Pezacki   +4 more
doaj   +1 more source

Bioorthogonal Reactions in Activity-Based Protein Profiling [PDF]

Molecules, 2020
Activity-based protein profiling (ABPP) is a powerful technique to label and detect active enzyme species within cell lysates, cells, or whole animals. In the last two decades, a wide variety of applications and experimental read-out techniques have been pursued in order to increase our understanding of physiological and pathological processes, to ...
Verhelst, Steven H.L., Bonger, Kimberly M., Willems, Lianne I.   +2 more
openaire   +6 more sources

Should I Stay or Should I Go-Leveraging an Overlooked Feature of Click-to-Release Chemistry for Affinity Labeling. [PDF]

Angew Chem Int Ed Engl
Click‐to‐release (C2R) chemistry is redirected from payload liberation toward ligand‐directed covalent protein labeling through a transient dihydropyridazine methide (DHP‐methide). This concept opens the way to dual‐functional systems combining covalent target engagement with controlled effector release.
Kern D, Németh K, Imre T, Simon J, Meggyes K, Bárdos G, Petri L, Bojtár M, Kormos A, Kele P.   +9 more
europepmc   +3 more sources

Recent Advances in Bioorthogonal Reactions

CHIMIA, 2019
The ability to selectively perform chemical reactions within living systems has transformed the field of Chemical Biology. These chemoselective processes have had a major scientific impact by enabling studies of cellular processes, producing tools for the ligations of large biomolecules, and advancing our ability to image or track small ...
Schäfer, Rebecca J.B., Aronoff, Matthew R., Wennemers, Helma   +2 more
openaire   +5 more sources

Improving bioorthogonal protein ubiquitylation by click reaction [PDF]

Bioorganic & Medicinal Chemistry, 2013
Posttranslational modification of proteins with ubiquitin (ubiquitylation) regulates numerous cellular processes. Besides functioning as a signal for proteasomal degradation, ubiquitylation has also non-proteolytic functions by altering the biochemical properties of the modified protein.
Schneider, Daniel, Schneider, Tatjana, Rösner, Daniel, Scheffner, Martin, Marx, Andreas   +4 more
openaire   +3 more sources

Diazo group as a new chemical reporter for bioorthogonal labelling of biomolecules† [PDF]

RSC Advances, 2014
Diazoacetyl groups have been shown to undergo spontaneous cycloaddition reactions with strained alkenes and alkynes. The rates of reaction are similar to the equivalent reactions of azide groups.
Laia Josa-Culleré, Yelena A. Wainman, Kevin M. Brindle, Finian J. Leeper   +3 more
doaj   +1 more source

Rational design for high bioorthogonal fluorogenicity of tetrazine‐encoded green fluorescent proteins

Natural Sciences, 2022
The development of bioorthogonal fluorogenic probes constitutes a vital force to advance life sciences. Tetrazine‐encoded green fluorescent proteins (GFPs) show high bioorthogonal reaction rate and genetic encodability but suffer from low fluorogenicity.
Longteng Tang, Riley M. Bednar, Nikita D. Rozanov, Marcus L. Hemshorn, Ryan A. Mehl, Chong Fang   +5 more
doaj   +1 more source