Results 21 to 30 of about 121,897 (203)

Heterologous Expression of the Unusual Terreazepine Biosynthetic Gene Cluster Reveals a Promising Approach for Identifying New Chemical Scaffolds

mBio, 2020
Advances in genome sequencing have revitalized natural product discovery efforts, revealing the untapped biosynthetic potential of fungi. While the volume of genomic data continues to expand, discovery efforts are slowed due to the time-consuming nature ...
Lindsay K. Caesar, Matthew T. Robey, Michael Swyers, Md N. Islam, Rosa Ye, Purav P. Vagadia, Gary E. Schiltz, Paul M. Thomas, Chengcang C. Wu, Neil L. Kelleher, Nancy P. Keller, Jin Woo Bok   +11 more
doaj   +1 more source

Multiplexed mobilization and expression of biosynthetic gene clusters

Nature Communications, 2022
AbstractBacterial genomes contain large reservoirs of biosynthetic gene clusters (BGCs) that are predicted to encode unexplored natural products. Heterologous expression of previously unstudied BGCs should facilitate the discovery of additional therapeutically relevant bioactive molecules from bacterial culture collections, but the large-scale ...
Vincent Libis, Logan W. MacIntyre, Rabia Mehmood, Liliana Guerrero, Melinda A. Ternei, Niv Antonovsky, Ján Burian, Zongqiang Wang, Sean F. Brady   +8 more
openaire   +3 more sources

Heterologous expression of pikromycin biosynthetic gene cluster using Streptomyces artificial chromosome system

Microbial Cell Factories, 2017
Background Heterologous expression of biosynthetic gene clusters of natural microbial products has become an essential strategy for titer improvement and pathway engineering of various potentially-valuable natural products.
Hye-rim Pyeon, Hee-Ju Nah, Seung-Hoon Kang, Si-Sun Choi, Eung-Soo Kim   +4 more
doaj   +1 more source

Characterization of the Ergosterol Biosynthesis Pathway in Ceratocystidaceae

Journal of Fungi, 2021
Terpenes represent the biggest group of natural compounds on earth. This large class of organic hydrocarbons is distributed among all cellular organisms, including fungi.
Mohammad Sayari, Magrieta A. van der Nest, Emma T. Steenkamp, Saleh Rahimlou, Almuth Hammerbacher, Brenda D. Wingfield   +5 more
doaj   +1 more source

Cloning of the Bisucaberin B Biosynthetic Gene Cluster from the Marine Bacterium Tenacibaculum mesophilum, and Heterologous Production of Bisucaberin B

Marine Drugs, 2018
The biosynthetic gene cluster for bisucaberin B (1, bsb gene cluster), an N-hydroxy-N-succinyl diamine (HSD)-based siderophore, was cloned from the marine bacterium Tenacibaculum mesophilum, originated from a marine sponge.
Masaki J. Fujita, Yusuke Goto, Ryuichi Sakai   +2 more
doaj   +1 more source

Biosynthetic gene cluster for the cladoniamides, bis-indoles with a rearranged scaffold. [PDF]

PLoS ONE, 2011
The cladoniamides are bis-indole alkaloids isolated from Streptomyces uncialis, a lichen-associated actinomycete strain. The cladoniamides have an unusual, indenotryptoline structure rarely observed among bis-indole alkaloids. I report here the isolation,
Katherine S Ryan
doaj   +1 more source

One Pathway, Two Cyclic Non-Ribosomal Pentapeptides: Heterologous Expression of BE-18257 Antibiotics and Pentaminomycins from Streptomyces cacaoi CA-170360

Microorganisms, 2021
The strain Streptomyces cacaoi CA-170360 produces the cyclic pentapeptides pentaminomycins A–H and BE-18257 A–C, two families of cyclopeptides synthesized by two non-ribosomal peptide synthetases encoded in tandem within the same biosynthetic gene ...
Fernando Román-Hurtado, Marina Sánchez-Hidalgo, Jesús Martín, Francisco Javier Ortiz-López, Daniel Carretero-Molina, Fernando Reyes, Olga Genilloud   +6 more
doaj   +1 more source

Activation of an unconventional meroterpenoid gene cluster in Neosartorya glabra leads to the production of new berkeleyacetals

Acta Pharmaceutica Sinica B, 2018
Fungal genomes carry many gene clusters seemingly capable of natural products biosynthesis, yet most clusters remain cryptic or down-regulated. Genome mining revealed an unconventional paraherquonin-like meroterpenoid biosynthetic gene cluster in the ...
Tao Zhang, Jun Wan, Zhajun Zhan, Jian Bai, Bingyu Liu, Youcai Hu   +5 more
doaj   +1 more source

Expanding our understanding of sequence-function relationships of type II polyketide biosynthetic gene clusters: bioinformatics-guided identification of Frankiamicin A from Frankia sp. EAN1pec. [PDF]

PLoS ONE, 2015
A large and rapidly increasing number of unstudied "orphan" natural product biosynthetic gene clusters are being uncovered in sequenced microbial genomes.
Yasushi Ogasawara, Benjamin J Yackley, Jacob A Greenberg, Snezna Rogelj, Charles E Melançon   +4 more
doaj   +1 more source

New Insights into Chloramphenicol Biosynthesis in Streptomyces venezuelae ATCC 10712 [PDF]

, 2014
Comparative genome analysis revealed seven uncharacterized genes, sven0909 to sven0915, adjacent to the previously identified chloramphenicol biosynthetic gene cluster (sven0916–sven0928) of Streptomyces venezuelae strain ATCC 10712 that was absent in a ...
Al-Bassam, Asghar, Aínsa, Bibb, Bibb, Brown, Bunet, Chang, Chiara Borsetto, Claesen, Copp, Datsenko, Doull, Gomez-Escribano, Govind Chandra, Gregory, Gross, Gust, Gust, Gust, Hanahan, He, Herrmann, Hong, Juan Pablo Gomez-Escribano, Kelley, Kieser, Lambalot, Latimer, Laureti, Lorena T. Fernández-Martínez, Lu, MacNeil, Mahmoud M. Al-Bassam, Makris, Maureen J. Bibb, Mervyn J. Bibb, Mosher, Owen, Pacholec, Paget, Piffaretti, Piraee, Puar, Punta, Sambrook, Sherwood, Shima, Stuttard, Tunca, Vining, Vining, Walsh, Yanai, Zerikly   +54 more
core   +1 more source