Results 31 to 40 of about 3,203,016 (289)

Heterologous expression of pikromycin biosynthetic gene cluster using Streptomyces artificial chromosome system

Microbial Cell Factories, 2017
Background Heterologous expression of biosynthetic gene clusters of natural microbial products has become an essential strategy for titer improvement and pathway engineering of various potentially-valuable natural products.
Hye-rim Pyeon, Hee-Ju Nah, Seung-Hoon Kang, Si-Sun Choi, Eung-Soo Kim   +4 more
doaj   +1 more source

Heterologous Expression of the Unusual Terreazepine Biosynthetic Gene Cluster Reveals a Promising Approach for Identifying New Chemical Scaffolds

mBio, 2020
Advances in genome sequencing have revitalized natural product discovery efforts, revealing the untapped biosynthetic potential of fungi. While the volume of genomic data continues to expand, discovery efforts are slowed due to the time-consuming nature ...
Lindsay K. Caesar, Matthew T. Robey, Michael Swyers, Md N. Islam, Rosa Ye, Purav P. Vagadia, Gary E. Schiltz, Paul M. Thomas, Chengcang C. Wu, Neil L. Kelleher, Nancy P. Keller, Jin Woo Bok   +11 more
doaj   +1 more source

MIBiG 2.0: a repository for biosynthetic gene clusters of known function [PDF]

, 2019
Fueled by the explosion of (meta)genomic data, genome mining of specialized metabolites has become a major technology for drug discovery and studying microbiome ecology.
Tracanna, V., Selem-Mojica, N., Weber, T., Navarro-Muñoz, J. C., van Santen, J. A., Lund, G., Medema, M. H., Terlouw, B. R., Collemare, J., Shaw, S., Andreu, V. P., Sisto, A. C., Duran, H. G. S., Charkoudian, L. K., Robinson, S. L., Epstein, S. C., Kautsar, S. A., Blin, K., van der Hooft, J. J. J., Alanjary, M., Linington, R. G.   +20 more
core   +1 more source

Alternative sigma factor over-expression enables heterologous expression of a type II polyketide biosynthetic pathway in Escherichia coli. [PDF]

PLoS ONE, 2013
BACKGROUND: Heterologous expression of bacterial biosynthetic gene clusters is currently an indispensable tool for characterizing biosynthetic pathways.
David Cole Stevens, Kyle R Conway, Nelson Pearce, Luis Roberto Villegas-Peñaranda, Anthony G Garza, Christopher N Boddy   +5 more
doaj   +1 more source

Designing and implementing an assay for the detection of rare and divergent NRPS and PKS clones in European, Antarctic and Cuban soils [PDF]

, 2015
The ever increasing microbial resistome means there is an urgent need for new antibiotics. Metagenomics is an underexploited tool in the field of drug discovery. In this study we aimed to produce a new updated assay for the discovery of biosynthetic gene
David A Pearce, Gregory C A Amos, Gregory C. A. Amos (799040), Leo Calvo-Bado (507757), Berry, Andrew E., Chiara Borsetto (799041), Andrew E Berry, Laskaris, Paris, Calvo-Bado, Leo, Paris Laskaris, Carlos Vallin (5665333), Berry, Andrew, Calvo-Bado, Leo A., David A. Pearce (189743), Leo Calvo-Bado, Elizabeth M H Wellington, Chiara Borsetto, Elizabeth M. H. Wellington (325617), Borsetto, Chiara, Kevin K Newsham, Amos, Gregory, Carlos Vallin, Paris Laskaris (325613), Andrew E. Berry (799043), Martin Krsek, Wellington, Elizabeth M. H., Martin Krsek (799042), Pearce, David, Pearce, David A., Kevin K. Newsham (352778), Vallin, Carlos, Krsek, Martin, Wellington, Elizabeth, Newsham, Kevin, Wellington, E. M. H., Amos, Gregory C. A., Newsham, Kevin K.   +36 more
core   +1 more source

Comparative genomics reveals phylogenetic distribution patterns of secondary metabolites in Amycolatopsis species

BMC Genomics, 2018
Background Genome mining tools have enabled us to predict biosynthetic gene clusters that might encode compounds with valuable functions for industrial and medical applications.
Martina Adamek, Mohammad Alanjary, Helena Sales-Ortells, Michael Goodfellow, Alan T. Bull, Anika Winkler, Daniel Wibberg, Jörn Kalinowski, Nadine Ziemert   +8 more
doaj   +1 more source

Biosynthetic Gene Content of the ‘Perfume Lichens’ Evernia prunastri and Pseudevernia furfuracea

Molecules, 2019
Lichen-forming fungi produce a vast number of unique natural products with a wide variety of biological activities and human uses. Although lichens have remarkable potential in natural product research and industry, the molecular mechanisms underlying ...
Anjuli Calchera, Francesco Dal Grande, Helge B. Bode, Imke Schmitt   +3 more
doaj   +1 more source

Identification of a Biosynthetic Gene Cluster in Rice for Momilactones [PDF]

Journal of Biological Chemistry, 2007
Rice diterpenoid phytoalexins such as momilactones and phytocassanes are produced in suspension-cultured rice cells treated with a chitin oligosaccharide elicitor and in rice leaves irradiated with UV light. The common substrate geranylgeranyl diphosphate is converted into diterpene hydrocarbon precursors via a two-step sequential cyclization and then ...
Kazuhiro, Shimura, Atsushi, Okada, Kazunori, Okada, Yusuke, Jikumaru, Kwang-Wook, Ko, Tomonobu, Toyomasu, Takeshi, Sassa, Morifumi, Hasegawa, Osamu, Kodama, Naoto, Shibuya, Jinichiro, Koga, Hideaki, Nojiri, Hisakazu, Yamane   +12 more
openaire   +2 more sources

Genomic clustering within functionally related gene families in Ascomycota fungi

Computational and Structural Biotechnology Journal, 2020
Multiple mechanisms collaborate for proper regulation of gene expression. One layer of this regulation is through the clustering of functionally related genes at discrete loci throughout the genome.
Danielle Hagee, Ahmad Abu Hardan, Juan Botero, James T. Arnone   +3 more
doaj   +1 more source

Genome Mining and Characterization of Biosynthetic Gene Clusters in Two Cave Strains of Paenibacillus sp.

Frontiers in Microbiology, 2021
The genome sequencing and mining of microorganisms from unexplored and extreme environments has become important in the process of identifying novel biosynthetic pathways. In the present study, the biosynthetic potential of Paenibacillus sp.
Jolanta Lebedeva, Gabriele Jukneviciute, Rimvydė Čepaitė, Vida Vickackaite, Raminta Pranckutė, Nomeda Kuisiene   +5 more
doaj   +1 more source