Results 71 to 80 of about 3,203,016 (289)

Sequence analysis of porothramycin biosynthetic gene cluster [PDF]

Folia Microbiologica, 2014
The biosynthetic gene cluster of porothramycin, a sequence-selective DNA alkylating compound, was identified in the genome of producing strain Streptomyces albus subsp. albus (ATCC 39897) and sequentially characterized. A 39.7 kb long DNA region contains 27 putative genes, 18 of them revealing high similarity with homologous genes from biosynthetic ...
Najmanova, Lucie, Ulanova, Dana, Jelinkova, Marketa, Kamenik, Zdenek, Kettnerova, Eliska, Koberska, Marketa, Gazak, Radek, Radojevic, Bojana, Janata, Jiri   +8 more
openaire   +2 more sources

Chassis optimization as a cornerstone for the application of synthetic biology based strategies in microbial secondary metabolism

Frontiers in Microbiology, 2015
The increased number of bacterial genome sequencing projects has generated over the last years a large reservoir of genomic information. In silico analysis of this genomic data has renewed the interest in bacterial bioprospecting for bioactive compounds ...
Tiago eBeites, Tiago eBeites, Marta Vaz Mendes, Marta Vaz Mendes   +3 more
doaj   +1 more source

Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis

FEBS Letters, EarlyView.
Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee, Junho Jeong, Chae Yeong Lee, Hyunjoon Cho, Man Hwan Oh, Je Chul Lee, Jee‐Young Lee, Yangmee Kim   +7 more
wiley   +1 more source

Phylogenetic Comparison of Swainsonine Biosynthetic Gene Clusters among Fungi

Journal of Fungi, 2022
Swainsonine is a cytotoxic alkaloid produced by fungi. Genome sequence analyses revealed that these fungi share an orthologous gene cluster, SWN, necessary for swainsonine biosynthesis.
Marwa Neyaz, Sumanjari Das, Daniel Cook, Rebecca Creamer   +3 more
doaj   +1 more source

Discerning protein pools by selective staining with self‐labeling tags

FEBS Letters, EarlyView.
Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley   +1 more source

MIBiG 4.0: advancing biosynthetic gene cluster curation through global collaboration [PDF]

Specialized or secondary metabolites are small molecules of biological origin, often showing potent biological activities with applications in agriculture, engineering and medicine.
Aguilar, C., Donahey, M. S. C., Teufel, R., Medema, M. H., Huynh, T., Jing, J., Katsuyama, Y., Zimmermann, C., Mahmud, T., Jayachandran, S., Streiff, A. B., Hooft, J. J. V. D., Barbieri, H. B., Macias, M. I., Liu, J., Santos-Medellin, C., Linington, R. G., Parra, J., Witte, T. E., Aleti, G., Augustijn, H. E., Barbosa, J. C. J., Koetsier, R. A., Borriss, R., Bulcock, B. W., Ryu, B., Bernaldo-Aguero, J., Creamer, K. E., Ostash, B. O., Tarnowski, M. J., Rubio, M. P., Kim, G. J., Hanif, N., Grayson, N. E., Bozhuyuk, K. A., Hindra, H., Kalkreuter, E., Kim, H., Rutz, A., Kakumu, Y., Li, S., Carrion, V. J., Choi, H., Barra, L., Larsen, N. J., Pham, H. T., Duncan, K. R., Damiani, T., Krug, D., Navarro, J., Robinson, S. L., Bhatt, K. M., Saldanha, M., Rosas-Becerra, L. R., Kollten, C., Gutierrez-Garcia, K., Haslinger, K., Ramesh, S., Singh, G., Connolly, J. A., Rojo, M. Y. A., Otani, H., Cumsille, A., Warnasinghe, R., Williams, S. E., Hoing, L., Korenskaia, A., Licona-Cassani, C., Merrild, A., Le, N., Singh, H., Kubach, N., Cano-Prieto, C., Rey, T., Kust, A., Reher, R., Zimmer, L., Gu, W., Strüder, A., Tan, B., Sowa, S. T., Truman, A. W., Doering, D. T., Chromy, J., Parade, D. S., Rosiles, P. Y., Salvi, L., Borges, R. M., Charria-Giron, E., Kang, K. B., Jenner, M., Sieber, S., Fewer, D. P., Tugizimana, F., Guerra, S., Machushynets, N. V., Torring, T., Yu, J., Paliya, S., Cediel-Becerra, J. D., Shah, A. D., Park, J., Lopez, J. A. V., Libis, V., Müller, A. V., Weber, T., Nguyen, G., Yang, D., Castro-Falcon, G., Dillen, J., Jung, J. J., Schoellhorn, S. M., Szenei, J., Ulbricht, C., Durusu, S., Singh, K. S., Yamada, K., Trindade, M., Frediansyah, A., Bielinski, V. A., Sokolova, N., Niedermeyer, T. H. J., Pan, X., Lorio, M., Flores, S. G., Weddeling, H. G., Hart, L., Surup, F., Cruz, A. A., Selem, N., Lee, B. T., Ossowicki, A., Kushnareva, A. K., Morgan, K. D., Kriukova, Y., Barrett, S. E., Kim, W., Gurevich, A., Rasmussen, M. H., Gavriilidou, A., Beemelmanns, C., Holland, D. C., Chooi, Y. H., Steffen, K., Sala, G. D., Foldi, J., Carter, R. S., Beqaj, D., Klapper, M., Wezel, G. P. V., Zdouc, M. M., Al-Salihi, S. A., Phan, C., Li, Y., Dallery, J., Launay, A. P., Owen, C., Winter, J. M., Schumm, C., Avelar-Rivas, J. A., Kruijff, M. D., Dommaraju, S. R., McBride, C. M., Chagas, F. O., Metsä-Ketela, M., Alharthi, S. A., Mantri, S., Alberti, F., Philmus, B., Hu, J., Helfrich, E. J., Bretscher, K. M., Fetter, A., Pidot, S. J., Gonzalez-Salazar, L. A., Piel, J., Lev, K. L., Bailey, C. B., Reitz, Z. L., Brigham, M. W., Blin, K., Larralde, M., McLellan, R. M., Arishi, A. A., Feuermann, M., Crusemann, M., Romero-Otero, A., Maleckis, M., Lien, A., Hrab, P., Booth, T. J., Jeong, E., Loureiro, C., Hemmann, J. L., Sonnenschein, E. C., Greco, C., Mast, Y., Biermann1, F., Berger, T., Buedenbender, L., Shishido, T. K., Lamber, J., Padva, L., Panghal, M., Damas-Ramos, L. C., Martin, B. D., Terlouw1, B. R., D’Agostino, P. M., Gomez-Escribano, J. P., Gentile, A., Mitchell, D. A., Williams, K., Breitenbach, M., Castelo-Branco, R., Capela, J., Kang, S., Rocca, S. M. W., Faussurier, B., Bader, C. D., Stegmann, E., Segato, L. G. B., Kovacs, A. T., Ortega, L. R. T., Lund, G., Bettenbuhl, S. M., Udwary, D. W., Axt, K. K., Martinez-Martinez, A. M., Hebra, T., Rice, A. J., Baunach, M., Sahadeo, L., Rond, T. D., Gomez, J. E. G., Tagirdzhanov, A., Sbaraini, N., Chaudhry, V., Rego, A., Sánchez-Carvajal, E., Chevrette, M. G., Maximo, M. F., Zhou, Z., Holme, J. E., Ulanova, D., Louwen, N. L., Charlop-Powers, Z., Walmsley, M., Collemare, J., Choi, Y., Gerke, J., Pourmohsenin, B., Ellerhorst, M., Gerstmans, H., Svenningsen, T., Sehnal, L., Horch, T., He, B., Rigolet, A., Kunjapur, A. M., O’Hare, J. H., Weir, A. N., Silviani, V., Choupannejad, R., Melkonian, C., Sosio, M., Tvilum, M., Vader, L., Akiyama, D. Y., Garza, E. A., Zimmermann, A., Avalon, N. E., Tong, Y., Rakhmanov, M., Kim, H. U., Ziemert, N., Lacovelli, R., Thome, N. U., Nguyen, H. T., Lftime, D., Egbert, S., Chaudhri, A. A., Pup, E. D., Lebeer, S., Lee, K., Sweeney, D., Alanjary, M., Hwang, J.   +286 more
core   +1 more source

Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network

FEBS Letters, EarlyView.
Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley   +1 more source

Secondary metabolites biosynthetic gene clusters in Aspergillus ustus.

, 2015
a. Two partial of such secondary biosynthetic gene cluster locate in the ends of scaffolds 43 and 37 of Aspergillus ustus draft assembly, respectively.Secondary metabolites biosynthetic gene clusters in Aspergillus ustus.
Dongliang Yu (514041), Xiaoming Song (695086), Hongye Li (695088), Borui Pi (514042), Yunsong Yu (496103), Fangwei Dai (695085), Congyi Zhu (695087)   +6 more
core   +1 more source

Biosynthetic Gene Cluster of Linaridin Peptides Contains Epimerase Gene

ChemBioChem, 2022
AbstractSalinipeptins belong to the type‐A linaridin class of ribosomally synthesized and post‐translationally modified peptides (RiPPs) comprising 22 amino acid residues with multiple D‐amino acids. Although chirality of other type‐A linaridins, such as grisemycin and cypemycin, has not been reported, the biosynthetic gene clusters of type‐A ...
Wanlu Xiao, Yasuharu Satoh, Yasushi Ogasawara, Tohru Dairi   +3 more
openaire   +3 more sources

In Vitro CRISPR/Cas9 System for Efficient Targeted DNA Editing

mBio, 2015
The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system, an RNA-guided nuclease for specific genome editing in vivo, has been adopted in a wide variety of organisms.
Yunkun Liu, Weixin Tao, Shishi Wen, Zhengyuan Li, Anna Yang, Zixin Deng, Yuhui Sun   +6 more
doaj   +1 more source