Results 51 to 60 of about 173,216 (260)
A sterol biosynthetic pathway in Mycobacterium
FEBS Letters, 1998 The genome sequence of Mycobacterium tuberculosis (and also M. leprae) revealed a significant number of homologies to Saccharomyces cerevisiae sterol biosynthetic enzymes. We addressed the hypothesis of a potential sterol biosynthetic pathway existing in Mycobacterium using cultures of Mycobacterum smegmatis.
Lamb, David C. +3 more
openaire +2 more sources
Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis
FEBS Letters, EarlyView.Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee +7 more
wiley +1 more source
FEBS Letters, EarlyView.Inositol pyrophosphates are energy‐rich signaling molecules that perform critical functions in cells. Three different families of phosphatases hydrolyze the β phosphate of the inositol pyrophosphate molecules: two have narrow specificities and one is promiscuous.
Ronda J. Rolfes
wiley +1 more source
Reconstructing enzyme evolution by protein engineering
FEBS Letters, EarlyView.Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler +2 more
wiley +1 more source
Discerning protein pools by selective staining with self‐labeling tags
FEBS Letters, EarlyView.Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley +1 more source
Advances in the Biosynthesis and Biomanufacturing of Carotenoid Cleavage Products
Natural Product CommunicationsCarotenoids are a class of natural pigments widely distributed in nature, which impart vibrant colors to plants, microorganisms, and marine organisms. Due to their unique isoprenoid structures and antioxidant properties, carotenoids have long been valued
Xuejian Zhang +7 more
doaj +1 more source
From reactants to products: computational methods for biosynthetic pathway design
Synthetic and Systems BiotechnologyOne of the main goals in synthetic biology is to produce value-added compounds from available precursors using enzymatic approaches. The construction of biosynthetic pathways for synthesizing target molecules plays a crucial role in this process. However,
Shaozhen Ding +7 more
doaj +1 more source
Biosynthesis of food constituents: Vitamins. 2. Water-soluble vitamins: Part 1 - a review
Czech Journal of Food Sciences, 2007 This review article gives a survey of the generally accepted biosynthetic pathways that lead to water-soluble vitamins in microorganisms, plants and some animals.
Jan Velíšek, Karel Cejpek
doaj +1 more source
Molecular Oncology, EarlyView.Dimethyl fumarate (DMF) reduces growth of HPV‐positive cervical cancer spheroids and induces ferroptosis in cervical cancer cells via blocking SLC7A11/Glutathione (GSH) axis. Combination of subcytotoxic doses of DMF and cisplatin (CDDP) further suppresses spheroid growth and drives cell death in 2D culture models.
Carolina Punziano +6 more
wiley +1 more source
Results in ChemistryThe extraction and modification of naturally-occurring compounds are widely performed due to their valuable medicinal and physical properties. Herin, we reported the extraction of bis-demethoxycurcumin from Ragbrai turmeric root.
Shayma M. Ahmad +9 more
doaj +1 more source