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Using circular RNAs to target toxic RNA-binding proteins in amyotrophic lateral sclerosis. [PDF]
Hollensen AK +4 more
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Northern blotting is the technique whereby RNA molecules are denatured, fractionated through agarose on the basis of size, and then transferred to a solid matrix for subsequent hybridization to a specific labeled probe. The technique can be used to analyze the pattern of transgene expression in an organism, and changes in that pattern following ...
J W, Pollard, C R, Perry, C F, Thurston
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Northern Blotting Analysis [PDF]
Northern blotting analysis is a classical method for analysis of the size and steady-state level of a specific RNA in a complex sample. In short, the RNA is size-fractionated by gel electrophoresis and transferred by blotting onto a membrane to which the RNA is covalently bound.
Josefsen, Knud, Nielsen, Henrik
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Analysis of RNA by Northern Blotting
Cold Spring Harbor Protocols, 2022Northern hybridization is used to measure the amount and size of RNAs transcribed from eukaryotic genes and to estimate their abundance. No other method is capable of obtaining these pieces of information simultaneously from a large number of RNA preparations; northern analysis is therefore fundamental to studies of gene expression in eukaryotic cells.
Michael R, Green, Joseph, Sambrook
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