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Detecting sRNAs by Northern Blotting
2011Small RNAs (sRNAs) play fundamental roles in modifying the transcriptomes and proteomes of organisms. sRNAs can be classified according to their origin and way of action into classes such as microRNAs, trans-acting siRNAs, heterochromatin siRNAs, Piwi-interacting RNAs, and natural antisense siRNAs, although microRNAs are the most conserved and studied ...
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Analysis of RNA by Northern-Blot Hybridization
2003Northern-blot hybridization, also referred to as Northern blotting, is one of several methods developed to detect the presence, to determine the size, and to quantify specific cellular mRNAs. By this method, total RNA or poly(A)(+)mRNA, prepared from the cells or tissue of interest, is fractionated by size on a denaturing agarose gel.
P E, Gallagher, D I, Diz
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Analysis of gene expression by Northern blot
Molecular Biotechnology, 1994In the analysis of gene expression, the steady-state level of RNA transcripts is one of the most convenient parameters used to monitor the activity of an endogenous or introduced gene in cell lines and tissues. A variety of methods, such as S1 hybridization, RNase protection and Northern blotting, can be used to measure RNA levels.
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Northern Blotting Techniques for Small RNAs
2010In eukaryotes, RNA silencing encompasses a range of biochemical processes mediated by approximately 20-25 nt small RNAs (smRNAs). This chapter describes northern blot hybridization techniques optimized for detection of such smRNAs, whether extracted from plant or animal tissues.
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The deep population history of northern East Asia from the Late Pleistocene to the Holocene
Cell, 2021John Olsen, Hucai Zhang, Tianyi Wang
exaly
Northern Blot Analysis of Gene Expression
2003In the analysis of gene expression, the steady-state level of RNA transcripts is one of the most convenient parameters used to monitor the activity of an endogenous or introduced gene in cell lines and tissues. A variety of methods, such as Sl hybridization (Chapter 21, this vol), RNase protection (Chapter 22), and Northern blotting, can be used to ...
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The role of northern peatlands in the global carbon cycle for the 21st century
Global Ecology and Biogeography, 2020Shushi Peng +2 more
exaly
Intensification and Driving Forces of Pastoralism in Northern China 5.7 ka Ago
Geophysical Research Letters, 2021Lele Ren, Xiaozhong Huang, Fahu Chen
exaly

