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Detection of MicroRNAs by Northern Blot
2023Small RNAs (sRNAs) are key regulators of transcriptomes and proteomes of organisms through their sequence-specific interaction with complementary RNA targets. sRNAs can be classified according to their origin and mode of action into different classes such as: microRNAs (miRNAs), small interfering RNAs (siRNAs) and PIWI-interacting RNAs (piRNAs).
Martinho, Claudia, Lopez-Gomollon, Sara
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BlotBase: A northern blot database
Gene, 2008With the availability of high-throughput gene expression analysis, multiple public expression databases emerged, mostly based on microarray expression data. Although these databases are of significant biomedical value, they do hold significant drawbacks, especially concerning the reliability of single gene expression profiles obtained by microarray ...
K, Schlamp +5 more
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Current Protocols in Toxicology, 2001
AbstractThis unit describes the processes of extracting RNA from tissues and analyzing it by northern blot hybridization to probe the expression of a particular gene at the transcriptional level.
M, Bergeron, J, Honkaniemi, F R, Sharp
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AbstractThis unit describes the processes of extracting RNA from tissues and analyzing it by northern blot hybridization to probe the expression of a particular gene at the transcriptional level.
M, Bergeron, J, Honkaniemi, F R, Sharp
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Nonradioactive Northern Blot of circRNAs
2018Circular RNAs (circRNAs) are recognized as a special species of transcripts in metazoans with increasing studies, and northern blotting is a direct way to confirm the existence and to evaluate the size of individual circRNAs. Northern blotting probes can be radioactive isotope (32P) labeled, which is not environment-friendly and sometimes inconvenient ...
Xiaolin, Wang, Ge, Shan
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Analysis of RNA by Northern Blot Hybridization
Current Protocols in Human Genetics, 2001AbstractSpecific sequences in RNA preparations can be detected by blotting and hybridization analysis using techniques very similar to those originally developed for DNA. Fractionated RNA is transferred from an agarose gel to a membrane support (northern blotting); unfractionated RNA is immobilized by slot or dot blotting.
T, Brown, K, Mackey
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