Results 71 to 80 of about 921 (145)

Image_2_Metabolic Implications of Using BioOrthogonal Non-Canonical Amino Acid Tagging (BONCAT) for Tracking Protein Synthesis.jpg

, 2020
BioOrthogonal Non-Canonical Amino acid Tagging (BONCAT) is a powerful tool for tracking protein synthesis on the level of single cells within communities and whole organisms.
Michael Dorle (8443950), Brian Tripet (8443944), Narayanaganesh Balasubramanian (1688254), Amanda Fuchs (8443947), Roland Hatzenpichler (487768), Valérie Copié (2738170), Berliza Soriano (8443956), Katherine F. Steward (8443941), Rachel Rawle (8443953), Brian Eilers (2849582), Brian Bothner (152279)   +10 more
core   +1 more source

BONCAT-SIP Metaproteomics

Syntrophic microbial consortia can contribute significantly to the activity and function of anoxic ecosystems, yet are often too rare to study their in situ physiologies using traditional molecular methods.
Skyler Friedline, Ryan Ziels
core   +1 more source

Role of the visual experience-dependent nascent proteome in neuronal plasticity

eLife, 2018
Experience-dependent synaptic plasticity refines brain circuits during development. To identify novel protein synthesis-dependent mechanisms contributing to experience-dependent plasticity, we conducted a quantitative proteomic screen of the nascent ...
Han-Hsuan Liu, Daniel B McClatchy, Lucio Schiapparelli, Wanhua Shen, John R Yates III, Hollis T Cline   +5 more
doaj   +1 more source

Subsurface hydrocarbon degradation strategies in low- and high-sulfate coal seam communities identified with activity-based metagenomics

npj Biofilms and Microbiomes, 2022
Environmentally relevant metagenomes and BONCAT-FACS derived translationally active metagenomes from Powder River Basin coal seams were investigated to elucidate potential genes and functional groups involved in hydrocarbon degradation to methane in coal
Hannah D. Schweitzer, Heidi J. Smith, Elliott P. Barnhart, Luke J. McKay, Robin Gerlach, Alfred B. Cunningham, Rex R. Malmstrom, Danielle Goudeau, Matthew W. Fields   +8 more
doaj   +1 more source

BONCAT enables time-resolved analysis of protein synthesis in native plant tissue [PDF]

, 2017
Proteomic plasticity undergirds stress responses in plants, and understanding such responses requires accurate measurement of the extent to which proteins levels are adjusted to counter external stimuli.
Ho, Samuel H., Sweredoski, Michael J., Glenn, Weslee S., Hess, Sonja, Stone, Shannon E., Moradian, Annie, Tirrell, David A., Bailey-Serres, Julia   +7 more
core  

Cell-selective proteomics reveal novel effectors secreted by an obligate intracellular bacterial pathogen

Nature Communications
Pathogenic bacteria secrete protein effectors to hijack host machinery and remodel their infectious niche. Rickettsia spp. are obligate intracellular bacteria that can cause life-threatening disease, but their absolute dependence on the host cell has ...
Allen G. Sanderlin, Hannah Kurka Margolis, Abigail F. Meyer, Rebecca L. Lamason   +3 more
doaj   +1 more source

Selective Proteomic Analysis of Antibiotic-Tolerant Cellular Subpopulations in Pseudomonas aeruginosa Biofilms

mBio, 2017
Biofilm infections exhibit high tolerance against antibiotic treatment. The study of biofilms is complicated by phenotypic heterogeneity; biofilm subpopulations differ in their metabolic activities and their responses to antibiotics.
Brett M. Babin, Lydia Atangcho, Mark B. van Eldijk, Michael J. Sweredoski, Annie Moradian, Sonja Hess, Tim Tolker-Nielsen, Dianne K. Newman, David A. Tirrell   +8 more
doaj   +1 more source

In‐Depth Cell‐Type‐Specific Proteome Landscape of the Brain from Human Amyloid‐β Overexpression Mouse Model

Advanced Science, Volume 12, Issue 20, May 29, 2025.
In situ Cell‐type‐specific proteome Analysis using antibody‐mediated Biotinylation (iCAB) is a method that directly targets and analyzes proteins in specific cell types within tissue. Applied to 5xFAD mice, iCAB identifies ≈8000 cell‐type‐specific proteins, revealing unique pathways in neurons, astrocytes, and microglia.
Taekyung Ryu, Seok‐Young Kim, Thujitha Thuraisamy, Jisu Shin, Yura Jang, Tae‐In Kam, Chan Hyun Na   +6 more
wiley   +1 more source

Time- and polarity-dependent proteomic changes associated with homeostatic scaling at central synapses

eLife, 2018
In homeostatic scaling at central synapses, the depth and breadth of cellular mechanisms that detect the offset from the set-point, detect the duration of the offset and implement a cellular response are not well understood.
Christoph T Schanzenbächer, Julian D Langer, Erin M Schuman   +2 more
doaj   +1 more source

Experimental Approaches to Visualize Effector Protein Translocation During Host‐Pathogen Interactions

BioEssays, Volume 47, Issue 4, April 2025.
Translocation of effector proteins is a key virulence trait of many pathogenic bacteria. Various strategies were developed to visualize the translocation of effector proteins, and this review summarizes experimental approaches for analyses of translocation towards localization, kinetics, and dynamics on single molecule level.
Verena Nadin Fritsch, Michael Hensel
wiley   +1 more source