Results 61 to 70 of about 921 (145)

Detection of protein-synthesizing microbes via bioorthogonal non-canonical amino acid tagging (BONCAT) v1

open access: yes, 2017
This protocol provides a detailed description of how to design and perform BONCAT experiments using two different bioorthogonal amino acids, L-azidohomoalanine (AHA) and L-homopropargylglycine (HPG), which are both surrogates of L-methionine.

core   +1 more source

Image_3_Metabolic Implications of Using BioOrthogonal Non-Canonical Amino Acid Tagging (BONCAT) for Tracking Protein Synthesis.jpg

open access: yes, 2020
BioOrthogonal Non-Canonical Amino acid Tagging (BONCAT) is a powerful tool for tracking protein synthesis on the level of single cells within communities and whole organisms.
Michael Dorle (8443950)   +10 more
core   +1 more source

Study of Oak Ridge soils using BONCAT-FACS-Seq reveals that a large fraction of the soil microbiome is active [PDF]

open access: yes, 2018
The ability to link soil microbial diversity to soil processes requires technologies that differentiate active subpopulations of microbes from so-called relic DNA and dormant cells. Measures of microbial activity based on various techniques including DNA
Sasse, Joelle   +7 more
core   +1 more source

Data_Sheet_1_Metabolic Implications of Using BioOrthogonal Non-Canonical Amino Acid Tagging (BONCAT) for Tracking Protein Synthesis.pdf

open access: yes, 2020
BioOrthogonal Non-Canonical Amino acid Tagging (BONCAT) is a powerful tool for tracking protein synthesis on the level of single cells within communities and whole organisms.
Michael Dorle (8443950)   +10 more
core   +1 more source

Chemoselective Characterization of New Extracellular Matrix Deposition in Bioengineered Tumor Tissues

open access: yesAdvanced Materials, Volume 37, Issue 47, November 27, 2025.
Understanding the bi‐directional crosstalk between cells and their surrounding extracellular matrix (ECM) is critical for tissue engineering. This study presents a chemoselective strategy for proteomic profiling of newly deposited ECM from resident cells.
Zihan Ling   +9 more
wiley   +1 more source

Detection of protein-synthesizing microorganisms in the environment via bioorthogonal non-canonical amino acid tagging (BONCAT) v1

open access: yes, 2015
This protocol provides a detailed description of how to design and perform BONCAT experiments using two different bioorthogonal amino acids, L-azidohomoalanine (AHA) and L-homopropargylglycine (HPG), which are both surrogates of L-methionine.
R. Hatzenpichler and V. J Orphan
core   +1 more source

Image_1_Metabolic Implications of Using BioOrthogonal Non-Canonical Amino Acid Tagging (BONCAT) for Tracking Protein Synthesis.jpg

open access: yes, 2020
BioOrthogonal Non-Canonical Amino acid Tagging (BONCAT) is a powerful tool for tracking protein synthesis on the level of single cells within communities and whole organisms.
Michael Dorle (8443950)   +10 more
core   +1 more source

Proteomic Insight Into Alzheimer's Disease Pathogenesis Pathways

open access: yesPROTEOMICS, Volume 25, Issue 21-22, Page 214-229, November 2025.
ABSTRACT Alzheimer's disease (AD) is a leading cause of dementia, but the pathogenesis mechanism is still elusive. Advances in proteomics have uncovered key molecular mechanisms underlying AD, revealing a complex network of dysregulated pathways, including amyloid metabolism, tau pathology, apolipoprotein E (APOE), protein degradation ...
Taekyung Ryu   +3 more
wiley   +1 more source

Detection of protein-synthesizing microorganisms in the environment via bioorthogonal non-canonical amino acid tagging (BONCAT) v1

open access: yes, 2017
This protocol provides a detailed description of how to design and perform BONCAT experiments using two different bioorthogonal amino acids, L-azidohomoalanine (AHA) and L-homopropargylglycine (HPG), which are both surrogates of L-methionine.

core   +1 more source

Exploring New Species of Lactic Acid Bacteria in Fermented Foods: A Case Study of Zha‐Chili

open access: yesFood Science &Nutrition, Volume 13, Issue 10, October 2025.
Recent novel LAB are primarily isolated from animal guts and fermented foods. Zha‐chili: a traditional Chinese fermented food as a novel LAB resource. The integration of metataxonomics, culturomics, genomics, high‐throughput methods, and artificial intelligence facilitates the discovery, detection, and characterization of novel LAB species.
Dingwu Qu   +5 more
wiley   +1 more source

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