Results 61 to 70 of about 921 (145)
This protocol provides a detailed description of how to design and perform BONCAT experiments using two different bioorthogonal amino acids, L-azidohomoalanine (AHA) and L-homopropargylglycine (HPG), which are both surrogates of L-methionine.
core +1 more source
BioOrthogonal Non-Canonical Amino acid Tagging (BONCAT) is a powerful tool for tracking protein synthesis on the level of single cells within communities and whole organisms.
Michael Dorle (8443950) +10 more
core +1 more source
Study of Oak Ridge soils using BONCAT-FACS-Seq reveals that a large fraction of the soil microbiome is active [PDF]
The ability to link soil microbial diversity to soil processes requires technologies that differentiate active subpopulations of microbes from so-called relic DNA and dormant cells. Measures of microbial activity based on various techniques including DNA
Sasse, Joelle +7 more
core +1 more source
BioOrthogonal Non-Canonical Amino acid Tagging (BONCAT) is a powerful tool for tracking protein synthesis on the level of single cells within communities and whole organisms.
Michael Dorle (8443950) +10 more
core +1 more source
Understanding the bi‐directional crosstalk between cells and their surrounding extracellular matrix (ECM) is critical for tissue engineering. This study presents a chemoselective strategy for proteomic profiling of newly deposited ECM from resident cells.
Zihan Ling +9 more
wiley +1 more source
This protocol provides a detailed description of how to design and perform BONCAT experiments using two different bioorthogonal amino acids, L-azidohomoalanine (AHA) and L-homopropargylglycine (HPG), which are both surrogates of L-methionine.
R. Hatzenpichler and V. J Orphan
core +1 more source
BioOrthogonal Non-Canonical Amino acid Tagging (BONCAT) is a powerful tool for tracking protein synthesis on the level of single cells within communities and whole organisms.
Michael Dorle (8443950) +10 more
core +1 more source
Proteomic Insight Into Alzheimer's Disease Pathogenesis Pathways
ABSTRACT Alzheimer's disease (AD) is a leading cause of dementia, but the pathogenesis mechanism is still elusive. Advances in proteomics have uncovered key molecular mechanisms underlying AD, revealing a complex network of dysregulated pathways, including amyloid metabolism, tau pathology, apolipoprotein E (APOE), protein degradation ...
Taekyung Ryu +3 more
wiley +1 more source
This protocol provides a detailed description of how to design and perform BONCAT experiments using two different bioorthogonal amino acids, L-azidohomoalanine (AHA) and L-homopropargylglycine (HPG), which are both surrogates of L-methionine.
core +1 more source
Exploring New Species of Lactic Acid Bacteria in Fermented Foods: A Case Study of Zha‐Chili
Recent novel LAB are primarily isolated from animal guts and fermented foods. Zha‐chili: a traditional Chinese fermented food as a novel LAB resource. The integration of metataxonomics, culturomics, genomics, high‐throughput methods, and artificial intelligence facilitates the discovery, detection, and characterization of novel LAB species.
Dingwu Qu +5 more
wiley +1 more source

