Results 81 to 90 of about 921 (145)

A Proteomics Approach to Profiling the Temporal Translational Response to Stress and Growth

iScience, 2018
Summary: To quantify dynamic protein synthesis rates, we developed MITNCAT, a method combining multiplexed isobaric mass tagging with pulsed SILAC (pSILAC) and bio-orthogonal non-canonical amino acid tagging (BONCAT) to label newly synthesized proteins ...
Daniel A. Rothenberg, J. Matthew Taliaferro, Sabrina M. Huber, Thomas J. Begley, Peter C. Dedon, Forest M. White   +5 more
doaj   +1 more source

Consistent prokaryotic successional dynamics across contrasting phytoplankton blooms

Limnology and Oceanography, Volume 70, Issue 2, Page 493-508, February 2025.
Abstract Heterotrophic prokaryotes play a vital role in organic matter cycling in the ocean and have been observed to undergo substrate‐controlled successions during phytoplankton blooms. However, there is limited understanding of the succession patterns during blooms triggered by upwelling events of different characteristics.
Markel Gómez‐Letona, Javier Arístegui, Ulf Riebesell, Marta Sebastián   +3 more
wiley   +1 more source

Cystatin B Involvement in Synapse Physiology of Rodent Brains and Human Cerebral Organoids

Frontiers in Molecular Neuroscience, 2019
Cystatin B (CSTB) is a ubiquitous protein belonging to a superfamily of protease inhibitors. CSTB may play a critical role in brain physiology because its mutations cause progressive myoclonic epilepsy-1A (EPM1A), the most common form of progressive ...
Eduardo Penna, Angela Cerciello, Angela Chambery, Rosita Russo, Filippo M. Cernilogar, Emilia Maria Pedone, Carla Perrone-Capano, Carla Perrone-Capano, Silvia Cappello, Rossella Di Giaimo, Rossella Di Giaimo, Marianna Crispino   +11 more
doaj   +1 more source

Protein Synthesis Inhibition and Activation of the c-Jun N-Terminal Kinase Are Potential Contributors to Cisplatin Ototoxicity

Frontiers in Cellular Neuroscience, 2017
Cisplatin has been regarded as an effective and versatile chemotherapeutic agent for nearly 40 years. Though the associated dose-dependent ototoxicity is known, the cellular mechanisms by which cochleovestibular hair cell death occur are not well ...
Brian D. Nicholas, Shimon Francis, Elizabeth L. Wagner, Sibo Zhang, Jung-Bum Shin   +4 more
doaj   +1 more source

Quantitative, Time-Resolved Proteomic Analysis Using Bio-Orthogonal Non-Canonical Amino Acid Tagging [PDF]

, 2015
Bio-orthogonal non-canonical amino acid tagging (BONCAT) is an analytical method that allows the selective analysis of the subset of newly synthesized cellular proteins produced in response to a biological stimulus.
Bagert, John David
core   +1 more source

Bioorthogonal non-canonical amino acid tagging to track transplanted human induced pluripotent stem cell-specific proteome

Stem Cell Research & Therapy
Background Human induced pluripotent stem cells (hiPSCs) and their differentiated cell types have a great potential for tissue repair and regeneration. While the primary focus of using hiPSCs has historically been to regenerate damaged tissue, emerging ...
Divya Sridharan, Julie A. Dougherty, Uzair Ahmed, Shridhar K. Sanghvi, Syed Baseeruddin Alvi, Ki Ho Park, Helena Islam, Sue E. Knoblaugh, Harpreet Singh, Elizabeth D. Kirby, Mahmood Khan   +10 more
doaj   +1 more source

Visualizing in situ translational activity for identifying and sorting slow-growing archaeal−bacterial consortia [PDF]

, 2016
To understand the biogeochemical roles of microorganisms in the environment, it is important to determine when and under which conditions they are metabolically active.
Woyke, Tanja, Orphan, Victoria J., Connon, Stephanie A., Malmstrom, Rex R, Hatzenpichler, Roland, Woykeb, Tanja, Goudeau, Danielle, Malmstrom, Rex R., Orphan, Victoria J, Connon, Stephanie A   +9 more
core   +1 more source

visualization of newly synthesized proteins in environmental microbes using amino acid tagging and click chemistry [PDF]

, 2014
Here we describe the application of a new click chemistry method for fluorescent tracking of protein synthesis in individual microorganisms within environmental samples.
Silvan Scheller, Brett M. Babin, Scheller, Silvan, Orphan, Victoria J., Patricia L. Tavormina, David A. Tirrell, Roland Hatzenpichler, Hatzenpichler, Roland, Victoria J. Orphan, Tavormina, Patricia L., Tirrell, David A., Babin, Brett M.   +11 more
core   +1 more source

Deoxynivalenol Affects Cell Metabolism and Increases Protein Biosynthesis in Intestinal Porcine Epithelial Cells (IPEC-J2): DON Increases Protein Biosynthesis

Toxins, 2018
Deoxynivalenol (DON) is a toxin found in cereals as well as in processed products such as pasta, and causes substantial economic losses for stock breeding as it induces vomiting, reduced feeding, and reduced growth rates in piglets.
Constanze Nossol, Peter Landgraf, Stefan Kahlert, Michael Oster, Berend Isermann, Daniela C. Dieterich, Klaus Wimmers, Sven Dänicke, Hermann-Josef Rothkötter   +8 more
doaj   +1 more source

Interrogating marine virus-host interactions and elemental transfer with BONCAT and nanoSIMS-based methods [PDF]

, 2018
While the collective impact of marine viruses has become more apparent over the last decade, a deeper understanding of virus-host dynamics and the role of viruses in nutrient cycling would benefit from direct observations at the single-virus level.
Kopf, Sebastian H., Guan, Yunbin, Sweredoski, Michael J., Thamatrakoln, Kimberlee, Orphan, Victoria J., Sullivan, Mathew B., Hess, Sonja, Bidle, Kay D., Poulos, Bonnie, Moradian, Annie, Pasulka, Alexis L.   +10 more
core