Results 41 to 50 of about 921 (145)
Conversion of Rutin, a Prevalent Dietary Flavonol, by the Human Gut Microbiota
Frontiers in Microbiology, 2020 The gut microbiota plays a pivotal role in the conversion of dietary flavonoids, which can affect their bioavailability and bioactivity and thereby their health-promoting properties. The ability of flavonoids to metabolically-activate the microbiota has,
Alessandra Riva +7 more
doaj +1 more source
Data in Brief, 2016 Here we provide data for SILAC and iTRAQ based hyperplexing combined with BONCAT based click chemistry for selective enrichment of newly synthesized proteins secreted by THP1 macrophages at various time points after infection with four different strains ...
Ajay Kumar +6 more
doaj +1 more source
Frontiers in Marine Science, 2022 Plastic debris reaching the ocean is exponentially increasing in parallel with plastic production. Once into seawater, plastic starts to leach organic compounds that are presumably additives and plastic oligomers, and that process is enhanced by solar ...
Cristina Romera-Castillo +3 more
doaj +1 more source
Acute Synthesis of CPEB Is Required for Plasticity of Visual Avoidance Behavior in Xenopus
Cell Reports, 2014 Neural plasticity requires protein synthesis, but the identity of newly synthesized proteins generated in response to plasticity-inducing stimuli remains unclear. We used in vivo bio-orthogonal noncanonical amino acid tagging (BONCAT) with the methionine
Wanhua Shen +6 more
doaj +1 more source
BONCAT in L. mexicana promastigotes.
, 2019 (A) Chemical structure of AHA, HPG and Methionine. (B) Workflow for BONCAT in L. mexicana promastigotes. AHA that can be bioorthogonally tagged with a fluorescent terminal alkyne was used for the BONCAT.
Paul W. Denny (6729614) +1 more
core +1 more source
LRG1 is an adipokine that promotes insulin sensitivity and suppresses inflammation
eLife, 2022 While dysregulation of adipocyte endocrine function plays a central role in obesity and its complications, the vast majority of adipokines remain uncharacterized.
Chan Hee J Choi +12 more
doaj +1 more source
, 2018 Experimental set up for BONCAT coupled with fluorophore addition via copper (I)- catalysed azide-alkyne cycloaddition (i.e., click chemistry) to detect the the transfer of HPG, a methionine analogue, from newly synthesized proteins in host cells to ...
Alexis Pasulka, Victoria J Orphan
core +1 more source
Site‐Specific Protein Bioconjugation Through Cellular Incorporation of Noncanonical Amino Acids
Angewandte Chemie International Edition, EarlyView.Genetic code expansion (GCE) enables site‐specific installation of noncanonical amino acids containing bioorthogonal conjugation handles, allowing precise, homogeneous protein modification. This review examines the principles of orthogonal translation, surveys the chemistries available for chemoselective labeling, and highlights emerging multi‐site ...
Rahul Sarkar +2 more
wiley +1 more source
Bioorthogoonalisilla ei-kanonisilla aminohapoilla leimauksen (BONCAT) ja kuparin katalysoiman ”click” reaktion hyödyntäminen proteiinisynteesin aloitusajankohdan määrittämisessä Methanosarcinassa substraatin metanolista asetaattiin vaihdon jälkeen [PDF]
, 2021 Biokaasun (metaanin) tuotanto asetaatista metanogeneesin kautta voisi olla energiatehokasta ja ekonomisesti kannattavaa. Metanogeenisten arkeoneiden, Methanosarcina, asetaatin kulutus ja asetaatin metabolinen reitti on hyvin ymmärretty, kuitenkin näiden ...
Haapanen, Mariia Micaela
core
Visualization of giant virus particles using BONCAT labeling and STED microscopy
, 2020 Giant DNA viruses of the phylum Nucleocytoviricota are being increasingly recognized as important regulators of natural protist populations. However, our knowledge of their infection cycles is still very limited due to a lack of cultured virus-host ...
Fischer, M. +3 more
core +1 more source