Results 21 to 30 of about 921 (145)

circPDE4B downregulation triggers GEMIN5‑dependent translational stress response and autophagy to reduce MAPT pathology. [PDF]

Alzheimers Dement
Abstract INTRODUCTION Circular RNAs (circRNAs) are emerging as key regulators of gene expression, synaptic plasticity, and neuronal function in Alzheimer's disease (AD). Here, we characterize the biological actions of circPDE4B, a highly expressed circRNA markedly reduced in AD. METHODS circPDE4B knockdown in neuronal progenitor cells was combined with
Puri S, van der Spek SJF, Lin M, Wang Z, Porter J, Hu J, Hu L, Rondón-Ortiz AN, Knyshov A, Libera JL, Counts SE, Lahiri DK, Tcw J, Emili A, Zhang X, Wolozin B.   +15 more
europepmc   +2 more sources

Translational activity is uncoupled from nucleic acid content in bacterial cells of the human gut microbiota

Gut Microbes, 2021
Changes in bacterial diversity in the human gut have been associated with many conditions, despite not always reflecting changes in bacterial activity. Methods linking bacterial identity to function are needed for improved understanding of how bacterial ...
Mariia Taguer, B. Jesse Shapiro, Corinne F. Maurice   +2 more
doaj   +1 more source

Microbial Community Response to Polysaccharide Amendment in Anoxic Hydrothermal Sediments of the Guaymas Basin

Frontiers in Microbiology, 2021
Organic-rich, hydrothermal sediments of the Guaymas Basin are inhabited by diverse microbial communities including many uncultured lineages with unknown metabolic potential.
Viola Krukenberg, Viola Krukenberg, Viola Krukenberg, Nicholas J. Reichart, Nicholas J. Reichart, Nicholas J. Reichart, Rachel L. Spietz, Rachel L. Spietz, Rachel L. Spietz, Roland Hatzenpichler, Roland Hatzenpichler, Roland Hatzenpichler   +11 more
doaj   +1 more source

Spatial Partitioning of Microbial Functional Potential Suggests Complex Nitrogen Cycling in a Subterranean Estuary. [PDF]

Environ Microbiol
Microbial communities in a Mediterranean subterranean estuary show spatially structured nitrogen‐cycling potential across hydrochemical zones. Microbial properties, more than environmental variables, predict NO2− and NH4+ concentrations, highlighting their key role in controlling nitrogen transformations prior to submarine groundwater discharge ...
Romano-Gude D, Arandia-Gorostidi N, Mena C, Diego-Feliu M, Almillategui B, Székely AJ, Bertilsson S, Bravo AG, Carrera J, Romera-Castillo C, Folch A, Garcia-Orellana J, Rodellas V, Ruiz-González C.   +13 more
europepmc   +2 more sources

Using BONCAT-FCM to Quantify Bacterial Production in Marine Observing Programs [PDF]

, 2022
Recent research has shown the potential of bioorthogonal noncanonical amino acid tagging (BONCAT) methods in studying microbial activity in marine environments in situ without the use of a radioisotope label.
McClure, Clay
core   +1 more source

Bioorthogonal Non-canonical Amino Acid Tagging Combined With Flow Cytometry for Determination of Activity in Aquatic Microorganisms

Frontiers in Microbiology, 2020
In this study, we have combined bioorthogonal non-canonical amino acid tagging (BONCAT) and flow cytometry (FCM) analysis, and we demonstrate the applicability of the method for marine prokaryotes.
Mathilde Lindivat, Aud Larsen, Aud Larsen, Ole Kristian Hess-Erga, Gunnar Bratbak, Ingunn Alne Hoell   +5 more
doaj   +1 more source

From Molecule to Meaning: Click and Bioorthogonal Chemical Reporters for Plant Systems, Biological Imaging, and Artistic Expression. [PDF]

Chembiochem
At the intersection of chemical biology, plant imaging, and contemporary art, this review introduces the concept of chembioart. By tracing how chemical reporters illuminate plant biomolecules in vivo, their role not only in advancing scientific understanding but also in inspiring transdisciplinary collaborations and visual expression is highlighted ...
Hinnebo M, Simon C, Tilouine A, Spriet C, Biot C.   +4 more
europepmc   +2 more sources

Bioorthogonal non-canonical amino acid tagging reveals translationally active subpopulations of the cystic fibrosis lung microbiota

Nature Communications, 2020
Bioorthogonal non-canonical amino acid tagging (BONCAT) coupled with flow cytometry (FACS) has been utilized to profile active microbiome of environmental samples.
Talia D. Valentini, Sarah K. Lucas, Kelsey A. Binder, Lydia C. Cameron, Jason A. Motl, Jordan M. Dunitz, Ryan C. Hunter   +6 more
doaj   +1 more source

BONCAT-based Profiling of Nascent Small and Alternative Open Reading Frame-encoded Proteins

Bio-Protocol, 2023
RIBO-seq and proteogenomics have revealed that mammalian genomes harbor thousands of unannotated small and alternative open reading frames (smORFs, <100 amino acids, and alt-ORFs, >100 amino acids, respectively).
Xiongwen Cao, Yanran Chen, Alexandra Khitun, Sarah Slavoff   +3 more
doaj   +1 more source

Sulfide Oxidation Products Support Microbial Metabolism at Interface Environments in a Marine-Like Serpentinizing Spring in Northern California. [PDF]

Geobiology
ABSTRACT Interface environments between extreme and neutrophilic conditions are often hotspots of metabolic activity and taxonomic diversity. In serpentinizing systems, the mixing of high pH fluids with meteoric water, and/or the exposure of these fluids to the atmosphere can create interface environments with distinct but related metabolic activities ...
Trutschel L, Kruger B, Czaja A, Brueck M, Sackett J, Druschel G, Rowe A.   +6 more
europepmc   +2 more sources