Detection, isolation and characterization of phage-host complexes using BONCAT and click chemistry [PDF]
Frontiers in MicrobiologyIntroductionPhages are viruses that infect prokaryotes and can shape microbial communities by lysis, thus offering applications in various fields. However, challenges exist in sampling, isolation and accurate prediction of the host specificity of phages ...
Udo Reichl +2 more
exaly +4 more sources
BONCAT-iTRAQ Labelling Reveals Molecular Markers of Adaptive Responses in Toxoplasma gondii to Pyrimethamine Treatment [PDF]
PathogensWe employed a BONCAT-iTRAQ labelling approach to investigate newly synthesised proteins (NSPs) in Toxoplasma gondii subjected to varying concentrations of the antifolate drug pyrimethamine.
John G. Mina +4 more
doaj +4 more sources
Flow Cytometric Analysis of Bacterial Protein Synthesis: Monitoring Vitality After Water Treatment [PDF]
Frontiers in Microbiology, 2021 Bacterial vitality after water disinfection treatment was investigated using bio-orthogonal non-canonical amino acid tagging (BONCAT) and flow cytometry (FCM).
Mathilde Lindivat +5 more
doaj +2 more sources
Pseudomonas aeruginosa dynamically prioritizes motility and resource recycling during prolonged starvation [PDF]
mSystemsHeterotrophic bacteria rapidly deplete essential macronutrients during growth and must navigate subsequent periods of growth arrest imposed by starvation.
Findlay D. Munro +3 more
doaj +2 more sources
Current Research in BiotechnologySelective identification of translationally active cells remains a key challenge in linking microbial function to population dynamics. Bioorthogonal non-canonical amino acid tagging (BONCAT) enables pulse-labelling of newly synthesized proteins in live ...
Yumechris Amekan +5 more
doaj +2 more sources
Lactulose selectively stimulates members of the gut microbiota, as determined by multi-modal activity-based sorting [PDF]
Gut MicrobesThere is much interest in the development of dietary supplements that selectively promote the growth of beneficial gut bacteria. The selectivity of many candidate prebiotics has, however, not been thoroughly investigated.
Hamid Rasoulimehrabani +12 more
doaj +2 more sources
Protocol for in vivo BONCAT labeling of nascent proteins in Xenopus laevis tadpole brains with high temporal precision for quantitative analysis [PDF]
STAR ProtocolsSummary: Proteostasis is critical for neuronal function and circuit plasticity. We present here an in vivo bio-orthogonal non-canonical amino acid tagging (BONCAT) protocol optimized to selectively label newly synthesized proteins in Xenopus laevis ...
Arifa Ahsan +4 more
doaj +2 more sources
A BONCAT-iTRAQ method enables temporally resolved quantitative profiling of newly synthesised proteins in Leishmania mexicana parasites during starvation. [PDF]
PLoS Neglected Tropical Diseases, 2019 Adaptation to starvation is integral to the Leishmania life cycle. The parasite can survive prolonged periods of nutrient deprivation both in vitro and in vivo.
Karunakaran Kalesh, Paul W Denny
doaj +2 more sources
Divergent mechanisms of active antibiotic resistance gene enrichment in soil driven by pesticide diversity [PDF]
Nature CommunicationsAntimicrobial resistance is an escalating global threat, with soils serving as reservoirs and conduits for the dissemination of antibiotic resistance genes (ARGs).
Yi-Fei Wang +11 more
doaj +2 more sources
Single-cell heterotrophic activity in deep-ocean prokaryotic communities quantified by BONCAT and microautoradiography. [PDF]
ISME CommunProkaryotes play a central role in marine biogeochemical cycles, yet quantifying their activity requires sensitive methods, particularly in the deep ocean where their biomass and metabolic rates are low.
Amano C +8 more
europepmc +2 more sources