Results 31 to 40 of about 921 (145)

Activity-Based Cell Sorting Reveals Resistance of Functionally Degenerate Nitrospira during a Press Disturbance in Nitrifying Activated Sludge

mSystems, 2021
Managing and engineering activated sludge wastewater treatment microbiomes for low-energy nitrogen removal requires process control strategies to stop the oxidation of ammonium at nitrite.
Maxwell B. W. Madill, Yaqian Luo, Pranav Sampara, Ryan M. Ziels   +3 more
doaj   +1 more source

Using click chemistry to study microbial ecology and evolution

ISME Communications, 2023
Technological advances have largely driven the revolution in our understanding of the structure and function of microbial communities. Culturing, long the primary tool to probe microbial life, was supplanted by sequencing and other -omics approaches ...
Sander van Kasteren, Daniel E. Rozen
doaj   +1 more source

Comparison of picolyl azide-based BONCAT and microautoradiography for assessing the heterotrophic prokaryotic activity in the deep ocean

Prokaryotes play a central role in marine biogeochemical cycles, yet quantifying their activity requires sensitive methods due to low biomass and metabolic rates, particularly in the deep ocean.
Amano C, Sintes E, Lebon N, Steiger J, Prijovic D, Reinthaler T, Obernosterer I, Bergauer K, Herndl GJ.   +8 more
europepmc   +2 more sources

Mass spectrometry proteomics for studying mitostasis. [PDF]

Protein Sci
Abstract Maintaining mitochondrial integrity and function is fundamental to cellular homeostasis. Cells rely on coordinated protein quality control (QC) systems—including intricate chaperone‐protease networks, the ubiquitin‐proteasome system, and cytosolic surveillance pathways—that together form a dynamic, cell‐wide mitostasis network governing the ...
Sharma L, Chakroborty S, Das H, Oeljeklaus S, Bender J, Warscheid B.   +5 more
europepmc   +2 more sources

Click Chemistry (CuAAC) and Detection of Tagged de novo Synthesized Proteins in Drosophila

Bio-Protocol, 2019
Copper-catalyzed azide-alkyne-cycloaddition (CuAAC), also known as ‘click chemistry’ serves as a technique for bio-orthogonal, that is, bio-compatible labeling of macromolecules including proteins or lipids.
Kathrin Marter, Oliver Kobler, Ines Erdmann, Elaheh Soleimanpour, Peter Landgraf, Anke Müller, Julia Abele, Ulrich Thomas, Daniela Dieterich   +8 more
doaj   +1 more source

In Old Mice, Exercise Induces Inflammation and Fibrosis Unless Alk5-Inhibitor and Oxytocin Are Used. [PDF]

J Cell Physiol
Muscle injury and exercise lead to repair and regeneration in young animals, but to inflammation and fibrosis in the old. The pathological responses to exercise were alleviated by Alk5i+oxytocin, each of which has positive effects, such as increased neurogenesis, myogenesis, immune homeostasis, and cardiac regeneration.
Cruz JMC, Yeung H, Alzalzalee R, Yang Q, Kabir H, McDonough SA, Mei X, Conboy MJ, Conboy IM.   +8 more
europepmc   +2 more sources

Using Click-Chemistry for Visualizing in Situ Changes of Translational Activity in Planktonic Marine Bacteria

Frontiers in Microbiology, 2017
A major challenge in microbial ecology is linking diversity and function to determine which microbes are actively contributing to processes occurring in situ.
Ainara Leizeaga, Margarita Estrany, Irene Forn, Marta Sebastián   +3 more
doaj   +1 more source

Biocompatible Chemistry: A Plug-and-Play Toolbox for Chemical Biology Research. [PDF]

Chembiochem
The “plug‐and‐play” nature of biocompatible chemistry makes it a powerful toolbox for investigating complicated biological systems and producing drug leads for clinical usages. This review summarizes the commonly used biocompatible reactions and the recent advances of their representative applications in chemical biology research, thus highlighting the
Lovato AR, Lin Z, Zheng Q.
europepmc   +2 more sources

Translatome analyses by bio-orthogonal non-canonical amino acid labeling reveal that MR1-activated MAIT cells induce an M1 phenotype and antiviral programming in antigen-presenting monocytes

Frontiers in Immunology, 2023
MAIT cells are multifunctional innate-like effector cells recognizing bacterial-derived vitamin B metabolites presented by the non-polymorphic MHC class I related protein 1 (MR1).
Josefine Jakob, Josefine Jakob, Josefine Jakob, Andrea Kröger, Andrea Kröger, Frank Klawonn, Dunja Bruder, Dunja Bruder, Lothar Jänsch   +8 more
doaj   +1 more source

Bioorthogonal Non-Canonical Amino Acid Tagging (BONCAT) to detect newly synthesized proteins in cells and their secretome [PDF]

PLoS ONE
Elizabeth P. Anim, Justin Mezzanotte, Siwei Chu, Ursula Stochaj   +3 more
doaj   +2 more sources