BONCAT-Live for isolation and cultivation of active environmental bacteria [PDF]
MBioIn diverse environments, microbes drive a myriad of processes, from geochemical and nutrient cycling to interspecies interactions, including associations with plants and animals.
Tatiana A Vishnivetskaya +2 more
exaly +5 more sources
Using BONCAT to dissect the proteome of S. aureus persisters [PDF]
MSphereBacterial persisters are a subpopulation of cells that exhibit a transient non-susceptible phenotype in the presence of bactericidal antibiotic concentrations. This phenotype can lead to the survival and regrowth of bacteria after treatment, resulting in
Bogdan Iulius Florea +2 more
exaly +6 more sources
BONCAT-FACS-Seq reveals the active fraction of a biocrust community undergoing a wet-up event [PDF]
Frontiers in Microbiology, 2023 Determining which microorganisms are active within soil communities remains a major technical endeavor in microbial ecology research. One promising method to accomplish this is coupling bioorthogonal non-canonical amino acid tagging (BONCAT) with ...
Marc W van Goethem +2 more
exaly +6 more sources
Probing the active fraction of soil microbiomes using BONCAT-FACS [PDF]
Nature Communications, 2019 Standard DNA-based analyses of microbial communities cannot distinguish between active microbes and dead or dormant cells. Here, Couradeau et al. use BONCAT (bioorthogonal non-canonical amino acid tagging), flow cytometry, and 16S rRNA gene amplicon ...
Joelle Schläpfer +2 more
exaly +3 more sources
Proteomic Profiling of Burkholderia thailandensis During Host Infection Using Bio-Orthogonal Noncanonical Amino Acid Tagging (BONCAT) [PDF]
Frontiers in Cellular and Infection Microbiology, 2018 Burkholderia pseudomallei and B. mallei are the causative agents of melioidosis and glanders, respectively, and are often fatal to humans and animals. Owing to the high fatality rate, potential for spread by aerosolization, and the lack of efficacious ...
Steven Branda
exaly +4 more sources
Dual-BONCAT reveals distinct subpopulations of anabolically active cells [PDF]
Applied and Environmental MicrobiologyBio-orthogonal non-canonical amino acid tagging (BONCAT) has emerged as a prominent molecular technique that enables microbial ecologists to visualize and identify metabolically active cells in cultures and complex microbial communities.
Dylan Mankel +5 more
doaj +2 more sources
Frontiers in Microbiology, 2020 BioOrthogonal Non-Canonical Amino acid Tagging (BONCAT) is a powerful tool for tracking protein synthesis on the level of single cells within communities and whole organisms.
Valerie Copie +2 more
exaly +3 more sources
Testing antimicrobial peptides inhibiting protein synthesis in living E. coli and K. pneumoniae using bio-orthogonal non-canonical amino-acid tagging [PDF]
Frontiers in MicrobiologyIntroductionAntimicrobial peptides (AMPs) inhibit bacteria through diverse mechanisms of action. While many AMPs exert their effects by interacting with and damaging bacterial membranes, a growing subset has been shown to target intracellular processes ...
Luigi de Pascale +10 more
doaj +2 more sources
Positive relationship between substrate-induced respiration rate and translationally active bacterial counts in soil [PDF]
mSystemsSoil microorganisms perform biogeochemical processes fundamental to soil functions. Bulk respiration, or microbial metabolic emission of CO2, is the classic indicator of soil biological activity.
Nina Rose Camillone +4 more
doaj +2 more sources
The activity of soil microbial taxa in the rhizosphere predicts the success of root colonization [PDF]
mSystemsPlant-beneficial microbes have great potential to improve sustainability in agriculture. Still, managing beneficial microbes is challenging because the impact of microbial dormancy on community assembly across the soil, rhizosphere, and endosphere is ...
Jennifer E. Harris +6 more
doaj +2 more sources