Ring-like oligomers of Synaptotagmins and related C2 domain proteins [PDF]
eLife, 2016 We recently reported that the C2AB portion of Synaptotagmin 1 (Syt1) could self-assemble into Ca2+-sensitive ring-like oligomers on membranes, which could potentially regulate neurotransmitter release.
Maria N Zanetti +7 more
doaj +10 more sources
Voltage-sensing phosphatase modulation by a C2 domain [PDF]
Frontiers in Pharmacology, 2015 The voltage-sensing phosphatase (VSP) is the first example of an enzyme controlled by changes in membrane potential. VSP has four distinct regions: the transmembrane voltage-sensing domain (VSD), the inter-domain linker, the cytosolic catalytic domain ...
Paul M. Castle +2 more
doaj +6 more sources
Molecular Analysis of Membrane Targeting by the C2 Domain of the E3 Ubiquitin Ligase Smurf1 [PDF]
Biomolecules, 2020 SMAD ubiquitination regulatory factor 1 (Smurf1) is a Nedd4 family E3 ubiquitin ligase that regulates cell motility, polarity and TGFβ signaling. Smurf1 contains an N-terminal protein kinase C conserved 2 (C2) domain that targets cell membranes and is ...
Jordan L. Scott +7 more
doaj +3 more sources
Structural basis of phosphatidylcholine recognition by the C2–domain of cytosolic phospholipase A2α [PDF]
eLife, 2019 Ca2+-stimulated translocation of cytosolic phospholipase A2α (cPLA2α) to the Golgi induces arachidonic acid production, the rate-limiting step in pro-inflammatory eicosanoid synthesis.
Yoshinori Hirano +8 more
doaj +3 more sources
Genome-Wide Analysis of the C2 Domain Family in Soybean and Identification of a Putative Abiotic Stress Response Gene GmC2-148 [PDF]
Frontiers in Plant Science, 2021 Plant C2 domain proteins play essential biological functions in numerous plants. In this study, 180 soybean C2 domain genes were identified by screening.
Yue Sun +12 more
doaj +2 more sources
C2‐domain containing calcium sensors in neuroendocrine secretion [PDF]
Journal of Neurochemistry, 2016 The molecular mechanisms for calcium‐triggered membrane fusion have long been sought for, and detailed models now exist that account for at least some of the functions of the many proteins involved in the process. Key players in the fusion reaction are a group of proteins that, upon binding to calcium, trigger the merger of cargo‐filled vesicles with ...
P. Pinheiro, S. Houy, J. B. Sørensen
semanticscholar +4 more sources
Purification of Cytosolic Phospholipase A2α C2-domain after Expression in Soluble Form in Escherichia coli [PDF]
Bio-Protocol, 2021 Previous expression/purification strategies for cytosolic phospholipase A2α C2-domain in Escherichia coli have relied on refolded protein recovered from inclusion bodies and sometimes containing C-terminal Cys139Ala and Cys141Ser substitutions to ...
Yoshinori Hirano +8 more
doaj +2 more sources
Structure of Blood Coagulation Factor VIII in Complex With an Anti-C2 Domain Non-Classical, Pathogenic Antibody Inhibitor [PDF]
Frontiers in Immunology, 2021 Factor VIII (fVIII) is a procoagulant protein that binds to activated factor IX (fIXa) on platelet surfaces to form the intrinsic tenase complex. Due to the high immunogenicity of fVIII, generation of antibody inhibitors is a common occurrence in ...
Estelle K. Ronayne +8 more
doaj +2 more sources
The Cytosolic Phospholipase A2α N-Terminal C2 Domain Binds and Oligomerizes on Membranes with Positive Curvature [PDF]
Biomolecules, 2020 Group IV phospholipase A2α (cPLA2α) regulates the production of prostaglandins and leukotrienes via the formation of arachidonic acid from membrane phospholipids. The targeting and membrane binding of cPLA2α to the Golgi involves the N-terminal C2 domain,
Katherine E. Ward +4 more
doaj +2 more sources
PTEN controls glandular morphogenesis through a juxtamembrane β-Arrestin1/ARHGAP21 scaffolding complex [PDF]
eLife, 2017 PTEN controls three-dimensional (3D) glandular morphogenesis by coupling juxtamembrane signaling to mitotic spindle machinery. While molecular mechanisms remain unclear, PTEN interacts through its C2 membrane-binding domain with the scaffold protein β ...
Arman Javadi +6 more
doaj +5 more sources