Genome-Wide Analysis of the C2 Domain Family in Soybean and Identification of a Putative Abiotic Stress Response Gene GmC2-148 [PDF]
Frontiers in Plant Science, 2021 Plant C2 domain proteins play essential biological functions in numerous plants. In this study, 180 soybean C2 domain genes were identified by screening.
Yue Sun +12 more
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Purification of Cytosolic Phospholipase A2α C2-domain after Expression in Soluble Form in Escherichia coli [PDF]
Bio-Protocol, 2021 Previous expression/purification strategies for cytosolic phospholipase A2α C2-domain in Escherichia coli have relied on refolded protein recovered from inclusion bodies and sometimes containing C-terminal Cys139Ala and Cys141Ser substitutions to ...
Yoshinori Hirano +8 more
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Structure of Blood Coagulation Factor VIII in Complex With an Anti-C2 Domain Non-Classical, Pathogenic Antibody Inhibitor [PDF]
Frontiers in Immunology, 2021 Factor VIII (fVIII) is a procoagulant protein that binds to activated factor IX (fIXa) on platelet surfaces to form the intrinsic tenase complex. Due to the high immunogenicity of fVIII, generation of antibody inhibitors is a common occurrence in ...
Estelle K. Ronayne +8 more
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The Cytosolic Phospholipase A2α N-Terminal C2 Domain Binds and Oligomerizes on Membranes with Positive Curvature [PDF]
Biomolecules, 2020 Group IV phospholipase A2α (cPLA2α) regulates the production of prostaglandins and leukotrienes via the formation of arachidonic acid from membrane phospholipids. The targeting and membrane binding of cPLA2α to the Golgi involves the N-terminal C2 domain,
Katherine E. Ward +4 more
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Molecular Analysis of Membrane Targeting by the C2 Domain of the E3 Ubiquitin Ligase Smurf1 [PDF]
Biomolecules, 2020 SMAD ubiquitination regulatory factor 1 (Smurf1) is a Nedd4 family E3 ubiquitin ligase that regulates cell motility, polarity and TGFβ signaling. Smurf1 contains an N-terminal protein kinase C conserved 2 (C2) domain that targets cell membranes and is ...
Jordan L. Scott +7 more
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The HRAS-binding C2 domain of PLCη2 suppresses tumor‐like synoviocytes and experimental arthritis in rheumatoid arthritis [PDF]
Experimental and Molecular MedicineFibroblast-like synoviocytes (FLSs), which are stromal cells that play key roles in rheumatoid arthritis (RA) pathophysiology, are characterized by a tumor-like phenotype and immunostimulatory actions.
Hyun Min Jeon +10 more
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Structural basis of phosphatidylcholine recognition by the C2–domain of cytosolic phospholipase A2α [PDF]
eLife, 2019 Ca2+-stimulated translocation of cytosolic phospholipase A2α (cPLA2α) to the Golgi induces arachidonic acid production, the rate-limiting step in pro-inflammatory eicosanoid synthesis.
Yoshinori Hirano +8 more
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Voltage-sensing phosphatase modulation by a C2 domain [PDF]
Frontiers in Pharmacology, 2015 The voltage-sensing phosphatase (VSP) is the first example of an enzyme controlled by changes in membrane potential. VSP has four distinct regions: the transmembrane voltage-sensing domain (VSD), the inter-domain linker, the cytosolic catalytic domain ...
Paul M. Castle +2 more
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Ring-like oligomers of Synaptotagmins and related C2 domain proteins [PDF]
eLife, 2016 We recently reported that the C2AB portion of Synaptotagmin 1 (Syt1) could self-assemble into Ca2+-sensitive ring-like oligomers on membranes, which could potentially regulate neurotransmitter release.
Maria N Zanetti +7 more
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Membrane binding and lipid-protein interaction of the C2 domain from coagulation factor V [PDF]
Current Research in Structural BiologyAnchoring of coagulation factors to anionic regions of the membrane involves the C2 domain as a key player. The rate of enzymatic reactions of the coagulation factors is increased by several orders of magnitude upon membrane binding. However, the precise
Y. Zenmei Ohkubo +3 more
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