Results 61 to 70 of about 38,034 (262)
Cells, 2022 Three-dimensional cell culture techniques mimic the in vivo cell environment more adequately than flat surfaces. Spheroids are multicellular aggregates and we aimed to produce scaffold-free spheroids of myogenic origin, called myospheres, using a mid ...
Katja Stange +3 more
doaj +1 more source
Tunable 3D‐Printed Static Mixers for Gradient Bioprinting With High Cell Viability
Advanced Materials Technologies, EarlyView.ABSTRACT The fabrication of native tissue‐like structures with gradual transitions in material properties, cell types, and growth factors remains a major challenge in biofabrication due to the lack of suitable methods. Mimicking the hierarchical organization of living tissues is essential for functional models, yet creating gradient, multimaterial ...
Florian Hofmann +9 more
wiley +1 more source
Topography‐Regulated Screening Platform for Myoblast Contact Guidance
Advanced Materials Technologies, EarlyView.This study develops a topography‐regulated screening platform (12 gradients in 96‐topography‐well plate) enabling analysis of skeletal muscle morphogenesis. The topography‐induced cell morphology changed, cell polarization, and myotube differentiation, establishing an evaluative framework for generating pathologically relevant myotube phenotypes to ...
Tianqi Feng +6 more
wiley +1 more source
Lactate overload inhibits myogenic activity in C2C12 myotubes [PDF]
Open Life Sciences, 2019 AbstractLactate (LA), an endogenous metabolite produced from pyruvate, can accumulate in skeletal muscle in certain conditions including major diseases, as well as during intensive exercise. Using differentiated C2C12 myotubes, we evaluated the early (1-h) and delayed (24-h) effects of LA (8 mM) on mechanisms involved in myogenesis or muscle atrophy ...
Oh Sarah Se-Jung +4 more
openaire +3 more sources
Irisin promotes C2C12 cell proliferation.
, 2019 (A, B) C2C12 cells were treated with irisin for indiciated times in culture medium (A) without FBS or (B) with FBS. Cell proliferation (%) was measured by MTS assay. Data are represented as the means ± SD (n = 3).
Jangho Lee (4742889) +4 more
core +1 more source
Characterization and differentiation of Fbxl21 KO C2C12 cells.
, 2022 (A) Loss of FBXL21 expression in Fbxl21 KO C2C12 cells compared to control cells. Successful Fbxl21 knockdown using the CRISPR-Cas9 was confirmed by immunofluorescence. Scale bars, 15 μm. (B) DAPI staining for Fig 3A. Scale bars, 15 μm. (C) Fusion index (
Collin M. Douglas (14305221) +11 more
core +1 more source
Frontiers in Cell and Developmental Biology, 2021 Muscle development requires myoblast differentiation and muscle fiber formation. Myod family inhibitor (Mdfi) inhibits myogenic regulatory factors in NIH3T3 cells, but how Mdfi regulates myoblast myogenic development is still unclear.
Bo Huang +7 more
doaj +1 more source
Advanced Materials Technologies, EarlyView.We introduce an additively manufactured bioreactor with a perfusion flow system and integrated temperature and pH sensors for skeletal muscle tissue biofabrication. The bioreactor's performance was evaluated by assessing the viability, spreading of the myoblast cells in a printed scaffold, and contraction of the isolated murine musculi interossei ...
Lys Sprenger +9 more
wiley +1 more source
Data on MyoD reduction by autophagy in C2C12 cells
Data in Brief, 2017 Autophagy is a highly regulated physiologic mechanism in which cells maintain homeostasis by degrading excessive or unnecessary proteins and damaged or aged organelles through the lysosomal machinery (Yorimitsu and Klionsky, 2005) [1]. MyoD is basic helix-loop-helix (bHLH) transcription factors that regulate myoblast proliferation and myogenic ...
Yeong-Min Yoo, Yung Chul Park
openaire +3 more sources
Trbp is required for C2C12 myoblast differentiation.
, 2016 (A) qRT-PCR of Trbp mRNA levels in siRNA-treated C2C12 cells (n = 3); (B) Representative images of C2C12 cells at differentiation day 3, stained with MF20 antibody.
Da-Zhi Wang (345525) +6 more
core +1 more source