Results 171 to 180 of about 37,904 (214)
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The Journal of Immunology, 1972
Abstract The C3b inactivator (C3bINA) contained in whole human serum has been purified by sequential diethylaminoethyl- and carboxymethyl-cellulose chromatography and polyacrylamide disc gel electrophoresis to yield a material which produces antibody to C3bINA when used to immunize rabbits.
Shaun Ruddy +2 more
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Abstract The C3b inactivator (C3bINA) contained in whole human serum has been purified by sequential diethylaminoethyl- and carboxymethyl-cellulose chromatography and polyacrylamide disc gel electrophoresis to yield a material which produces antibody to C3bINA when used to immunize rabbits.
Shaun Ruddy +2 more
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Molecular Immunology, 1999
Amplification of complement activation in blood and serum starts on multi-protein complexes that act as precursors of an alternative C3 convertase. Among these covalently linked C4b-, C3b-, and IgG-containing complexes C3b-C3b-IgG complexes represent the major species containing C3b and IgG.
Jelezarova E, Lutz HU
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Amplification of complement activation in blood and serum starts on multi-protein complexes that act as precursors of an alternative C3 convertase. Among these covalently linked C4b-, C3b-, and IgG-containing complexes C3b-C3b-IgG complexes represent the major species containing C3b and IgG.
Jelezarova E, Lutz HU
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Action of the C3b-inactivator on the cell-bound C3b.
Journal of immunology (Baltimore, Md. : 1950), 1979The action of C3bINA and beta 1H on cell-bound C3b is described in this paper. The alpha-polypeptide of C3b that binds covalently to cell surfaces is cleaved by the C3bINA and beta 1H into two fragments: one of 60,000 (C3b alpha-60) and another of 40,000 (C3b alpha-40) daltons. The beta-chain of C3b is unaffected by the C3bINA and beta 1H.
S K, Law, D T, Fearon, R P, Levine
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A fluorometric method for determination of C3b inactivator
Clinica Chimica Acta, 1982A simple method for the determination of C3b inactivator in human plasma was investigated. Fluorescent-labeled C3, which was prepared by treatment with methylamine and a fluorescent thiol reagent, N-(dimethylamino-4-methylcoumarinyl)-maleimide, was used as the substrate.
T, Seya, S, Nagasawa
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Interaction of C3 and C3b with immunoglobulin G
Molecular Immunology, 1983Human C3b as well as native C3 were found to bind to solid phase human and rabbit IgG. Haemolytically active C3 had significantly higher binding capacity to IgG than the C3b fragment. Inhibition experiments proved that C3 and C3b have common binding sites on the Fab and Fc part of the IgG molecule but the character of these binding sites was different.
J, Kulics +3 more
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C3b-Mediated chemiluminescence of human lymphocytes
Bulletin of Experimental Biology and Medicine, 1989Data are presented concerning peculiarities of human lymphocyte luminol-dependent chemiluminescence stimulated with C3b-opsonized and native zymosan. The response was maximal by the 60th min and 4-17 exceeded the results of the negative control. The C3b-opsonization considerably potentiated the lymphocyte activating zymosan's properties and the rate of
A N, Maianskiĭ +2 more
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Immobilization of C3b with retention of functional activity
Journal of Immunological Methods, 1990We compared eight commercially available, pre-activated affinity chromatography supports for ability to immobilize C3b that would retain functional activity. Pre-activated supports that we studied were: cyanogen bromide activated agarose, N-hydroxysuccinimide activated agarose, Reacti-Gel HW-65, Actigel A aldehyde activated agarose, thiopropyl ...
R E, Edens, R J, Linhardt, J M, Weiler
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The Journal of Immunology, 1979
Abstract A sensitive radio assay was developed to evaluate and quantitate the function of the control proteins important in the cleavage of cell-bound C3b and in the release of the subfragment C3c. In addition, the effects of the control proteins on C3b functional hemolytic activity and on C3b immune adherence reactivity were measured ...
T A, Gaither, C H, Hammer, M M, Frank
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Abstract A sensitive radio assay was developed to evaluate and quantitate the function of the control proteins important in the cleavage of cell-bound C3b and in the release of the subfragment C3c. In addition, the effects of the control proteins on C3b functional hemolytic activity and on C3b immune adherence reactivity were measured ...
T A, Gaither, C H, Hammer, M M, Frank
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C3b Acceptors on macrophages: Inhibition of Fcγ-receptor-mediated phagocytosis by acceptor-bound C3b
Immunology Letters, 1983The binding of nascent human C3b (i.e. the fragment of C3 just after trypsin cleavage) to mouse peritoneal macrophages was demonstrated by immune adherence. Acceptor-bound C3b could be detected longer than 24 h on the cell membrane. The rosette formation and phagocytosis of SRBC coated with anti-SRBC rat IgG was inhibited by preincubation of the cells ...
Z, Fábry, A, Erdei, J, Gergely
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Receptor-Binding Sites on C3 and C3b
The Journal of Immunology, 1977Abstract Human erythrocytes carry C3b receptors; Daudi lymphoid cells carry exclusively C3d receptors. With these two types of cells, it could be shown that isolated, uncleaved human C3 and soluble C3b possess two stable binding sites: SBS1-specific C3b receptors, and SBS2-specific for C3d receptors.
M P, Dierich, V A, Bokisch
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