Results 11 to 20 of about 101,738 (257)

Calmodulin-Binding Protein Detection Using a Non-Radiolabeled Calmodulin Fusion Protein

BioTechniques, 2001
Calmodulin-binding proteins are involved in numerous cellular signaling pathways. The biotinylated-calmodulin overlay is a nonradioactive method widely used to detect calmodulin-binding proteins in tissue and cell samples.
Gloria Murray, Mathew J. Marshall, William Trumble, Bernadene A. Magnuson   +3 more
doaj   +1 more source

Transcriptome Analysis of Melocactus glaucescens (Cactaceae) Reveals Metabolic Changes During in vitro Shoot Organogenesis Induction

Frontiers in Plant Science, 2021
Melocactus glaucescens is an endangered cactus highly valued for its ornamental properties. In vitro shoot production of this species provides a sustainable alternative to overharvesting from the wild; however, its propagation could be improved if the ...
Gabriela Torres-Silva, Ludmila Nayara Freitas Correia, Diego Silva Batista, Andréa Dias Koehler, Sheila Vitória Resende, Elisson Romanel, Daniela Cassol, Ana Maria Rocha Almeida, Susan R. Strickler, Chelsea Dvorak Specht, Wagner Campos Otoni   +10 more
doaj   +1 more source

Detection of Calmodulin-Binding Proteins Using a 32P-Labeled GST-Calmodulin Fusion Protein and a Novel Renaturation Protocol

BioTechniques, 1996
To identify calmodulin-binding proteins in cellular extracts and tissue homogenates and to analyze purified calmodulin target proteins, overlay procedures using 125I-calmodulin or, more recently, nonradioactive biotinylated calmodulin have been widely ...
Roland Fischer, Yu Wei, Martin Berchtold
doaj   +1 more source

Tilianin improves cognition in a vascular dementia rodent model by targeting miR-193b-3p/CaM- and miR-152-3p/CaMKIIα-mediated inflammatory and apoptotic pathways

Frontiers in Immunology, 2023
IntroductionAlthough vascular dementia (VaD) is the second most prevalent form of dementia, there is currently a lack of effective treatments. Tilianin, isolated from the traditional drug Dracocephalum moldavica L., may protect against ischemic injury by
Ting Sun, Linjie Tan, Mimin Liu, Li Zeng, Kaiyue Zhao, Zhongdi Cai, Shengnan Sun, Zhuorong Li, Rui Liu   +8 more
doaj   +1 more source

Kv7 channels can function without constitutive calmodulin tethering.

PLoS ONE, 2011
M-channels are voltage-gated potassium channels composed of Kv7.2-7.5 subunits that serve as important regulators of neuronal excitability. Calmodulin binding is required for Kv7 channel function and mutations in Kv7.2 that disrupt calmodulin binding ...
Juan Camilo Gómez-Posada, Paloma Aivar, Araitz Alberdi, Alessandro Alaimo, Ainhoa Etxeberría, Juncal Fernández-Orth, Teresa Zamalloa, Meritxell Roura-Ferrer, Patricia Villace, Pilar Areso, Oscar Casis, Alvaro Villarroel   +11 more
doaj   +1 more source

W342F Mutation in CCaMK Enhances Its Affinity to Calmodulin But Compromises Its Role in Supporting Root Nodule Symbiosis in Medicago truncatula

Frontiers in Plant Science, 2017
The calcium/calmodulin-dependent protein kinase (CCaMK) is regulated by free Ca2+ and Ca2+-loaded calmodulin. This dual binding is believed to be involved in its regulation and associated physiological functions, although direct experimental evidence for
Edgard Jauregui, Liqun Du, Liqun Du, Cynthia Gleason, B. W. Poovaiah   +4 more
doaj   +1 more source

Effect of Chronic Hydrogen Peroxide Exposure on Ion Transport in Gills of Common Carp (Cyprinus carpio)

Fishes, 2023
High environmental hydrogen peroxide (H2O2) has been demonstrated to be toxic for fish. However, the response mechanism of fish to chronic H2O2 exposure is not yet well understood.
Yating Mou, Bing Li, Yiran Hou, Rui Jia, Jian Zhu   +4 more
doaj   +1 more source

Interactions of Calmodulin With the Multiple Binding Sites of Cav1.2 Ca2+ Channels

Journal of Pharmacological Sciences, 2010
Although calmodulin binding to various sites of the Cav1.2 Ca2+ channel has been reported, the mechanism of the interaction is not fully understood. In this study we examined calmodulin binding to fragment channel peptides using a semi-quantitative pull ...
Hadhimulya Asmara, Etsuko Minobe, Zahangir A. Saud, Masaki Kameyama   +3 more
doaj   +1 more source

Functional, genetic and bioinformatic characterization of a calcium/calmodulin kinase gene in Sporothrix schenckii

BMC Microbiology, 2007
Background Sporothrix schenckii is a pathogenic, dimorphic fungus, the etiological agent of sporotrichosis, a subcutaneous lymphatic mycosis. Dimorphism in S.
Rodriguez-del Valle Nuri, Gonzalez-Mendez Ricardo R, Valentin-Berrios Shirley, Valle-Aviles Liz   +3 more
doaj   +1 more source

Human Calmodulin Mutations [PDF]

Frontiers in Molecular Neuroscience, 2018
Fluxes of calcium (Ca2+) across cell membranes enable fast cellular responses. Calmodulin (CaM) senses local changes in Ca2+ concentration and relays the information to numerous interaction partners. The critical role of accurate Ca2+ signaling on cellular function is underscored by the fact that there are three independent CaM genes (CALM1-3) in the ...
Jensen, Helene H, Brohus, Malene, Nyegaard, Mette, Overgaard, Michael T   +3 more
openaire   +5 more sources