Results 61 to 70 of about 349,942 (303)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Relationship between different subpopulations of circulating CD4+ T lymphocytes and microvascular or systemic oxidative stress in humans

Blood Pressure, 2017
Background and objective: Different components of the immune system, including innate and adaptive immunity (T effector lymphocytes and T regulatory lymphocytes – TREGs) may be involved in the development of hypertension, vascular injury and inflammation.
Carolina De Ciuceis, Claudia Agabiti-Rosei, Claudia Rossini, Paolo Airò, Mirko Scarsi, Angela Tincani, Guido Alberto Massimo Tiberio, Silvia Piantoni, Enzo Porteri, Leonardo Solaini, Sarah Duse, Francesco Semeraro, Beatrice Petroboni, Luigi Mori, Maurizio Castellano, Alice Gavazzi, Enrico Agabiti-Rosei, Damiano Rizzoni   +17 more
doaj   +1 more source

Intracapillary leucocyte accumulation as a novel antihaemorrhagic mechanism in acute pancreatitis in mice [PDF]

, 2009
Background: Pancreatic infiltration by leucocytes represents a hallmark in acute pancreatitis. Although leucocytes play an active role in the pathophysiology of this disease, the relation between leucocyte activation, microvascular injury and haemorrhage
Deduchovas, O., Hartwig, W., Kerkadze, V., Märten, A., Parseliunas, A., Ryschich, E., Salnikova, O., Schmidt, J., Sperandio, Markus   +8 more
core   +1 more source

Multi‐omics and low‐input proteomics profiling reveals dynamic regulation driving pluripotency initiation in early mouse embryos

FEBS Open Bio, EarlyView.
Mouse pre‐implantation development involves a transition from totipotency to pluripotency. Integrating transcriptomics, epigenetic profiling, low‐input proteomics and functional assays, we show that eight‐cell embryos retain residual totipotency features, whereas cytoskeletal remodeling regulated by the ubiquitin‐proteasome system drives progression ...
Wanqiong Li, Xi Xiao, Lingbin Qi, Chanyi Li, Sirui Song, Jiaying Qin, Zhigang Xue, Jinfeng Xue, Huaifang Li   +8 more
wiley   +1 more source

Deep learning-based classification of the capillary ultrastructure in human skeletal muscles

Frontiers in Molecular Biosciences
BackgroundCapillary ultrastructure in human skeletal muscles is dynamic and prone to alterations in response to many stimuli, e.g., systemic pathologies such as diabetes mellitus and arterial hypertension.
Marius Reto Bigler, Oliver Baum
doaj   +1 more source

Engineering of a complex bone tissue model with endothelialised channels and capillary-like networks [PDF]

European Cells & Materials, 2018
In engineering of tissue analogues, upscaling to clinically-relevant sized constructs remains a significant challenge. The successful integration of a vascular network throughout the engineered tissue is anticipated to overcome the lack of nutrient and ...
BJ Klotz, KS Lim, YX Chang, BG Soliman, I Pennings, FPW Melchels, TBF Woodfield, AJWP Rosenberg, J Malda, D Gawlitta   +9 more
doaj   +1 more source

On production and asymmetric focusing of flat electron beams using rectangular capillary discharge plasmas

, 2017
A method for the asymmetric focusing of electron bunches, based on the active plasma lensing technique is proposed. This method takes advantage of the strong inhomogeneous magnetic field generated inside the capillary discharge plasma to focus the ...
Bagdasarov, G. A., Barber, S. K., Bobrova, N. A., Boldarev, A. S., Bulanov, S. S., Bulanov, S. V., Esarey, E., Gasilov, V. A., Gonsalves, A. J., Kando, M., Korn, G., Leemans, W. P., Levato, T., Margarone, D., Olkhovskaya, O. G., Sasorov, P. V., Schroeder, C. B., van Tilborg, J.   +17 more
core   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

THE CAPILLARY PATTERN IN HUMAN MASSETER MUSCLE DURING AGEING

Image Analysis and Stereology, 2013
The effect of ageing on the capillary network in skeletal muscles has produced conflicting results in both, human and animals studies. Some of the inconsistencies are due to non-comparable and biased methods that were applied on thin transversal sections,
Erika Cvetko, Jiří Janáček, Lucie Kubínová, Ida Eržen   +3 more
doaj   +1 more source

Enhanced self-healing capacity in cementitious materials by use of encapsulated carbonate precipitating bacteria : from proof-of-concept to reality [PDF]

, 2013
In this study, two bacteria-based self-healing systems were developed for the proof-of-concept and approach to a realistic self-healing. A self-healing system with glass capillaries and silica sol gel carried bacterial cells was first built.
De Belie, Nele, Verstraete, Willy, Wang, Jianyun   +2 more
core