Results 51 to 60 of about 4,965,597 (147)

Tolerance to mutations in the foot-and-mouth disease virus integrin-binding RGD region is different in cultured cells and in vivo and depends on the capsid sequence context. [PDF]

, 2008
Engineered RNAs carrying substitutions in the integrin receptor-binding Arg-Gly-Asp (RGD) region of foot-and-mouth disease virus (FMDV) were constructed (aa 141-147 of VP1 capsid protein) and their infectivity was assayed in cultured cells and suckling
Gutiérrez-Rivas, M, Baranowski, Eric, Sáiz, M, M. Gutierrez-Rivas, Sobrino, Francisco, F. Sobrino, M. Saiz, Sobrino Castelló, Francisco, Gutiérrez-Rivas, Monica, Pulido, Miguel R, M. R. Pulido, Sobrino, F, Pulido, Mr, Sáiz, Margarita, E. Baranowski, Rodríguez Pulido, Miguel Ramón   +15 more
core   +2 more sources

Engineering a highly durable adeno-associated virus receptor for analytical applications

Molecular Therapy: Methods & Clinical Development, 2023
Adeno-associated virus (AAV) is a major viral vector used in gene therapy. There are multiple AAV serotypes, and many engineered AAV serotypes are developed to alter their tissue tropisms with capsid modification.
Kouhei Yoshida, Yuji Tsunekawa, Kento Kurihara, Kazuya Watanabe, Yuriko Makino-Manabe, Mikako Wada, Toru Tanaka, Teruhiko Ide, Takashi Okada   +8 more
doaj   +1 more source

The DNA Ejection Process in Bacteriophage λ [PDF]

, 2007
Bacteriophages have long served as model systems through which the nature of life may be explored. From a physical or mechanical point of view, phages are excellent examples of natural nanotechnology: they are nanometer-scale systems which depend ...
Grayson, Paul Daniel
core   +1 more source

Quantifying Nanoscale Properties of Engineered Virus Capsids for Malaria Vaccines [PDF]

Biophysical Journal, 2016
An important challenge in targeted drug delivery and vaccine development is to create the most effective interface between the carrier and target cells. Parasitic diseases, for which no approved vaccines are available, remain a world health problem with over one million severe cases per year attributed to malaria alone.
Jin, Albert J., Mertz, David, Liao, Hsien-Shun, Johri, Aanchal, Torres, Luis, Wu, Yimin, Narum, David   +6 more
openaire   +1 more source

Altering VP1 and VP2 expression in trans affects the transduction efficiency of AAV9

Frontiers in Bioengineering and Biotechnology
Currently, adeno-associated virus (AAV) is one of the most reliable carrier for gene delivery in both proliferating and non-proliferating cells. Stable and long-lasting transgene expression has made this viral vector a key platform for the development of
Maxim K. Efremov, Alima Galieva, Andrew N. Brovin, Natalia V. Mesonzhnik, Mikhail B. Afonin, Elena N. Subcheva, Alexander Karabelsky   +6 more
doaj   +1 more source

Modified AAV5 capsid for improved brain biodistribution following direct injection in preclinical models

Molecular Therapy: Methods & Clinical Development
Gene therapies based on adeno-associated virus vectors hold strong potential for the treatment of central nervous system disorders. However, systemic delivery is limited by the blood-brain barrier, off-target effects, immune responses, and vector loss ...
Sebastian N. Kieper, Elisabeth A. Spronck, Meriem Bourajjaj, Roberto D.V.S. Morais, M. Leontien van der Bent, Seyda Acar Broekmans, Charlot van Rooijen, Rhodé van Westen, Giorgia Squeri, Giso Brasser, Morgane Wartel, Stijn J.H. Waaijer, C. Warner Hoornenborg, Tycho M. Hoogland, Richard Porter, Anggakusuma   +15 more
doaj   +1 more source

Structural basis for biologically relevant mechanical stiffening of a virus capsid by cavity-creating or spacefilling mutations

Scientific Reports, 2017
Recent studies reveal that the mechanical properties of virus particles may have been shaped by evolution to facilitate virus survival. Manipulation of the mechanical behavior of virus capsids is leading to a better understanding of viral infection, and ...
Pablo Guerra, Alejandro Valbuena, Jordi Querol-Audí, Cristina Silva, Milagros Castellanos, Alicia Rodríguez-Huete, Damià Garriga, Mauricio G. Mateu, Nuria Verdaguer   +8 more
doaj   +1 more source

αFAP-specific nanobodies mediate a highly precise retargeting of modified AAV2 capsids thereby enabling specific transduction of tumor tissues

Molecular Therapy: Methods & Clinical Development
Due to the refractiveness of tumor tissues to adeno-associated virus (AAV) transduction, AAV vectors are poorly explored for cancer therapy delivery.
Olaniyi Olarewaju, Franziska Held, Pamela Curtis, Cynthia Hess Kenny, Udo Maier, Tadas Panavas, Francois du Plessis   +6 more
doaj   +1 more source

Generation of a bovine cell line for gene engineering using an HIV-1-based lentiviral vector

Scientific Reports, 2022
Human immunodeficiency virus type 1 (HIV-1)-based lentiviral vectors are indispensable tools for gene engineering in mammalian cells. Conversely, lentiviral vector transduction is severely inhibited in bovine cells.
Nanami Morizako, Erika P. Butlertanaka, Yuri L. Tanaka, Honoka Shibata, Tamaki Okabayashi, Hirohisa Mekata, Akatsuki Saito   +6 more
doaj   +1 more source

A Rationally Designed AAV9-DM Capsid with Minimal Liver Tropism

Cells
Adeno-associated viral vectors (AAV) are the leading gene therapy in the clinic. AAV9 has been of particular interest due to its wide tropism for multiple tissue types as well as being able to cross the blood-brain barrier and transduce central nervous ...
Zoe C. Nabakowski, Izabella C. Jaramillo, Primrose Tanachaiwiwat, Geoffrey D. Keeler   +3 more
doaj   +1 more source