Results 71 to 80 of about 31,732 (250)

Programmable Nanobody‐Targeting Chimeras Enable Intracellular Viral Protein Degradation

Advanced Science, EarlyView.
Nab‐TAC enables targeted degradation of HBV surface antigen (HBsAg) in vivo. By fusing nanobody‐based recognition domains with programmable proteasome‐recruiting degradation signals, Nab‐TAC reduces HBsAg levels in a hydrodynamic HBV mouse model. ABSTRACT Chronic hepatitis B virus (HBV) infection remains a major global health challenge, largely because
Max Yu‐Chen Pan, Ting‐Hui Lee, Tzu‐Ning Peng, Eva Yi‐Hsuan Wu, Yu‐Ting Chiu, Chih‐Chien Chiu, Hong Vinh Nguyen, Yuan‐Shao Pao, Ming‐Hong Chao, Richard Kuan‐Lin Lee, Han‐Chung Wu, Lily Hui‐Ching Wang   +11 more
wiley   +1 more source

Untangling Inositol (Pyro)Phosphate Biology Through Emerging Technologies

Advanced Science, EarlyView.
Inositol (pyro)phosphates represent a vital class of intracellular messengers that govern multiple biological processes. The last decade has experienced a surge of interest in the functions of this class of signaling molecules, primarily due to the introduction of several innovative analytical technologies.
Adolfo Saiardi, Mengsi Lu, Yue Zhang, Anuj Shukla, An‐Li Andrea Ko, Henning J. Jessen   +5 more
wiley   +1 more source

Computation and Structure‐Guided Arginine Scanning Engineers a Hyperactive AP Endonuclease for Multiplex Viral RNA Sensing

Advanced Science, EarlyView.
Computational and structure‐guided arginine scanning rewires the DNA‐binding interface of APE1 to create APE1‐Evo, a hyperactive yet specific AP endonuclease. Integrated into the NAPTUNE‐V2.0 cascade, APE1‐Evo enables amplification‐free, multiplex viral RNA sensing for dengue virus and influenza A/B, highlighting a general strategy for engineering ...
Junlan Wang, Ting Wu, Feizuo Wang, Boyang Wang, Xin Yu Wong, Senfeng Zhang, Shengsheng Ma, Yao Liu, Fangyao Yao, Zhifeng Zeng, Siyuan Yang, Kunming Liu, Qi Wang, Yilong Wang, Yunxiang Zang, Xiaolong Wang, Xingxu Huang, Shao Li, Quanquan Ji, Tao Hu, Chunyi Hu   +20 more
wiley   +1 more source

Bone-targeting AAV-mediated silencing of Schnurri-3 prevents bone loss in osteoporosis

Nature Communications, 2019
The adaptor protein SHN3 suppresses new bone formation by controlling osteoblast activity. Here, the authors show that ablation of SHN3 function, either genetically or by delivering an artificial miRNA via AAV9, rescues bone loss in osteoporotic mice ...
Yeon-Suk Yang, Jun Xie, Dan Wang, Jung-Min Kim, Phillip W. L. Tai, Ellen Gravallese, Guangping Gao, Jae-Hyuck Shim   +7 more
doaj   +1 more source

Engineered MS2 Virus Capsids for Cellular Display of Peptide Antigens

ACS Chemical Biology
ABSTRACT Our ability to respond to emerging pandemics and pathogen resistance relies critically on our ability to build vaccines quickly and efficiently. In this report we used an efficient enzymatic oxidative coupling reaction to create a viral capsid-based vaccine platform that is modular and quickly adaptable for ...
Hannah S. Martin, Paul Huang, Ian C. Leifer, Preeta Pratakshya, Matthew B. Francis   +4 more
openaire   +2 more sources

Overcoming Transmission Barriers: A Dual‐Functional Adhesive Hydrogel for Direct Monkeypox Virus Neutralization and Wound Healing

Advanced Science, EarlyView.
MPXV infection leads to persistent viral replication and severe local inflammation, while existing treatments cannot simultaneously remove viruses and repair injured tissue. This study constructs Polymer@mPDA@Gel hydrogel via a two‐step hierarchical assembly method: mPDA anchors photothermal polymers to form antiviral photothermal subunits, which are ...
Ming Zhang, Jiaxin Tian, Xiaolong Zhu, Xiao Li, Xiangshu Qiu, Yuanyuan Li, Ben Zhong Tang, Ning Shi   +7 more
wiley   +1 more source

301. AAV Capsid Engineering to Improve Transduction in Retina and Brain

Molecular Therapy, 2016
Gene therapy vectors based on adeno-associated virus (AAV) are currently in clinical studies for numerous disease indications including Leber's congenital amaurosis, age-related macular degeneration, hemophilia, muscular dystrophy and Parkinson's disease. AAV vectors hold considerable promise as therapeutic agents; however there is potential to further
Jennifer Sullivan, Lisa Stanek, Michael Lukason, Elizabeth Barry, Shayla Russell, James Morris, Bryan Mastis, Anna Alves, Jie Bu, Lamya S. Shihabuddin, Seng Cheng, Abraham Scaria   +11 more
openaire   +1 more source

Targeting CXCR2 in Nociceptive Sensory Neurons Offers Novel Therapeutic Potentials for Postoperative Pain

Advanced Science, EarlyView.
Tissue incision induces CXCL5 release, which activates neuronal CXCR2 on incision‐innervating sensory neurons. Neuronal CXCR2 couples with TRPA1 to drive mechanical pain and engages p38 MAPK signaling to upregulate TRPV1 and promote heat pain. Targeted Cxcr2 knockdown relieves incisional pain while preserving CXCR2‐dependent neutrophil responses ...
Yushuang Pan, Peiyi Li, Yufeng Chen, Zishan Dong, Yuanyuan Li, Boyu Liu, Yan Tai, Chuan Wang, Jianqiao Fang, Chengyu Yin, Boyi Liu   +10 more
wiley   +1 more source

Structural characterization and epitope mapping of the AAVX affinity purification ligand

Molecular Therapy: Methods & Clinical Development
The application of adeno-associated virus (AAV) vectors in human gene therapies requires reproducible and homogeneous preparations for clinical efficacy and safety.
Mario Mietzsch, Manasi Kamat, Kari Basso, Paul Chipman, Juha T. Huiskonen, Robert McKenna   +5 more
doaj   +1 more source

Reversible loading of thiol-modified curcumin in an engineered protein capsid

RSC Advances, 2017
The dodecahedral capsid formed by Aquifex aeolicus lumazine synthase (AaLS) is a promising protein scaffold for bionanotechnological applications.
Q. Guo, G. C. Thomas, K. J. Woycechowsky
openaire   +2 more sources