Results 51 to 60 of about 252,228 (286)

Inter-Conversion of carbohydrate reserves from pollen maturation to rehydration in a chili pepper [PDF]

, 2013
Carbohydrate metabolism is critical for male fertility. Carbohydrate reserves (soluble sugars and starch) were quantified in three advanced stages of pollen development, from the final maturation to rehydration, in a chili pepper cultivar (Capsicum ...
Carrizo Garcia, Carolina, Guarnieri, Massimo, Pacini, Ettore   +2 more
core   +1 more source

Exploration of Trichoderma reesei as an alternative host for erythritol production

Biotechnology for Biofuels and Bioproducts
Background Erythritol, a natural polyol, is a low-calorie sweetener synthesized by a number of microorganisms, such as Moniliella pollinis. Yet, a widespread use of erythritol is limited by high production costs due to the need for cultivation on glucose-
Audrey Masi, Georg Stark, Johanna Pfnier, Robert L. Mach, Astrid R. Mach-Aigner   +4 more
doaj   +1 more source

Cell wall target fragment discovery using a low‐cost, minimal fragment library

FEBS Letters, EarlyView.
LoCoFrag100 is a fragment library made up of 100 different compounds. Similarity between the fragments is minimized and 10 different fragments are mixed into a single cocktail, which is soaked to protein crystals. These crystals are analysed by X‐ray crystallography, revealing the binding modes of the bound fragment ligands.
Kaizhou Yan, Mathew Stanley, Olawale Raimi, Andrew T. Ferenbach, Helge C Dorfmueller, Daan M. F. van Aalten   +5 more
wiley   +1 more source

Structure and activity of the Streptococcus pyogenes family GH1 6-phospho β-glycosidase, Spy1599 [PDF]

, 2013
The group A streptococcus Streptococcus pyogenes is the causative agent of a wide spectrum of invasive infections, including necrotizing fasciitis, scarlet fever and toxic shock syndrome.
Aguilar-Moncayo, Ajdić, Cantarel, Collin, Conejo, Cowtan, Cowtan, Edward J. Taylor, Emsley, Evans, Fujita, Gideon J. Davies, Gregg, Harry Brumer, Henrissat, Henrissat, Hondorp, Jens M. Eklof, Jerome Dabin, Jin, Johan P. Turkenburg, Judith Stepper, Kabsch, Kietzman, Kori, Leslie, McNicholas, Murshudov, Padilla, Pancholi, Prachumporn Kongsaeree, Preeyanuch Thongpoo, Ryan, Ryan, Seshadri, Shelburne, Shelburne, Shelburne, Sheldon, Suck, Suits, Vagin, Wiesmann, Wiesmann, Winn, Winter, Wright, Zechel   +47 more
core   +1 more source

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

Graph theoretic methods for the analysis of structural relationships in biological macromolecules [PDF]

, 2005
Subgraph isomorphism and maximum common subgraph isomorphism algorithms from graph theory provide an effective and an efficient way of identifying structural relationships between biological macromolecules.
Altschul, Artymiuk, Artymiuk, Artymiuk, Artymiuk, Artymiuk, Barnard, Baxevanis, Benning, Berman, Bernstein, Brint, Brint, Bron, Bruno, Bryant, Crandell, Dean, Diestel, Doubet, Fan, Feizi, Figueras, Flores, Gardiner, Gati, Good, Gray, Groves, Gruer, Gund, Hagadone, Harrison, Holden, Hutchinson, Jasanoff, Johnson, Kanna, Klausner, Kleywegt, Koch, Kraulis, Lengauer, Lesk, Martin, Martin, McGregor, Messmer, Mitchell, Ollis, Pickering, Ray, Raymond, Read, Salton, Samudrala, Sayle, Simon, Srere, Sussenguth, Tesmer, Tinoco, Trinajstic, Tsukada, Ullmann, van Rijsbergen, Willett, Willett, Willett, Willett, Williams, Wilson, Zhang   +72 more
core   +3 more sources

Poplar Carbohydrate-Active Enzymes. Gene Identification and Expression Analyses [PDF]

Plant Physiology, 2006
Abstract Over 1,600 genes encoding carbohydrate-active enzymes (CAZymes) in the Populus trichocarpa (Torr. & Gray) genome were identified based on sequence homology, annotated, and grouped into families of glycosyltransferases, glycoside hydrolases, carbohydrate esterases, polysaccharide lyases, and expansins.
Jane, Geisler-Lee, Matt, Geisler, Pedro M, Coutinho, Bo, Segerman, Nobuyuki, Nishikubo, Junko, Takahashi, Henrik, Aspeborg, Soraya, Djerbi, Emma, Master, Sara, Andersson-Gunnerås, Björn, Sundberg, Stanislaw, Karpinski, Tuula T, Teeri, Leszek A, Kleczkowski, Bernard, Henrissat, Ewa J, Mellerowicz   +15 more
openaire   +2 more sources

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Comparative analysis of carbohydrate active enzymes in Clostridium termitidis CT1112 reveals complex carbohydrate degradation ability.

PLoS ONE, 2014
Clostridium termitidis strain CT1112 is an anaerobic, gram positive, mesophilic, cellulolytic bacillus isolated from the gut of the wood-feeding termite, Nasutitermes lujae.
Riffat I Munir, John Schellenberg, Bernard Henrissat, Tobin J Verbeke, Richard Sparling, David B Levin   +5 more
doaj   +1 more source

Sugar conformations that enhance cleavage of glycosidic bonds in carbohydrate-active enzymes [PDF]

, 2015
Carbohydrate-active enzymes select particular conformations of one sugar of their carbohydrate substrates to enhance catalysis. The evolution of this conformation during catalysis, known as the catalytic itinerary, is of fundamental interest in ...
Raich, Lluís, Rovira, Carme
core