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Archives of Virology, 1998
The 3'-terminal 1500 nucleotides of the genome of pelargonium flower break carmovirus (PFBV) were sequenced from RT-PCR amplification products. One large ORF was found, encoding a 345 amino acid protein of Mr 37 kDa, which corresponds to the coat protein, as confirmed by immunoprecipitation of products of in vitro transcription and translation.
R, Berthomé +5 more
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The 3'-terminal 1500 nucleotides of the genome of pelargonium flower break carmovirus (PFBV) were sequenced from RT-PCR amplification products. One large ORF was found, encoding a 345 amino acid protein of Mr 37 kDa, which corresponds to the coat protein, as confirmed by immunoprecipitation of products of in vitro transcription and translation.
R, Berthomé +5 more
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Journal of Phytopathology, 1997
AbstractA polymerase chain reaction (PCR)‐based assay was used to detect pelargonium flower break carmovirus (PFBV) in total RNA extractions made from infected Pelargonium plants. Extracts were reverse transcribed (RT) and the resultant cDNA was amplified by PCR, using oligonucleotide primers specific for 343, 510 and 832 base pair fragments of the RNA‐
A. Franck, G. Loebenstein, A. Gera
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AbstractA polymerase chain reaction (PCR)‐based assay was used to detect pelargonium flower break carmovirus (PFBV) in total RNA extractions made from infected Pelargonium plants. Extracts were reverse transcribed (RT) and the resultant cDNA was amplified by PCR, using oligonucleotide primers specific for 343, 510 and 832 base pair fragments of the RNA‐
A. Franck, G. Loebenstein, A. Gera
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Archives of Virology, 2011
A virus associated with yellow-to-purple ringspot on honeysuckle plants has been detected and tentatively named honeysuckle ringspot virus (HnRSV). The complete nucleotide sequence of HnRSV from infected honeysuckle has been determined. The genomic RNA of HnRSV is 3,956 nucleotides in length and is predicted to contain five open reading frames (ORFs ...
Anju, Gulati-Sakhuja +3 more
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A virus associated with yellow-to-purple ringspot on honeysuckle plants has been detected and tentatively named honeysuckle ringspot virus (HnRSV). The complete nucleotide sequence of HnRSV from infected honeysuckle has been determined. The genomic RNA of HnRSV is 3,956 nucleotides in length and is predicted to contain five open reading frames (ORFs ...
Anju, Gulati-Sakhuja +3 more
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Archives of Virology, 2000
The genome of Japanese iris necrotic ring virus (JINRV) consists of a positive-sense ssRNA of 4014 nucleotides with six major open reading frames (ORFs). A 5'-non-coding region of 31 nucleotides precedes the first initiation codon. Like Carnation mottle virus (CarMV), the 5'-proximal three ORFs encode a 26 kDa protein (p26) and two readthrough proteins,
Y, Takemoto +4 more
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The genome of Japanese iris necrotic ring virus (JINRV) consists of a positive-sense ssRNA of 4014 nucleotides with six major open reading frames (ORFs). A 5'-non-coding region of 31 nucleotides precedes the first initiation codon. Like Carnation mottle virus (CarMV), the 5'-proximal three ORFs encode a 26 kDa protein (p26) and two readthrough proteins,
Y, Takemoto +4 more
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EPPO Bulletin, 1995
With the aim of optimizing the detection of pelargonium flower‐break carmovirus in Pelargonium×hortorum by immunosorbent electron microscopy (ISEM), several factors were investigated. The best results were obtained when we used NPPI‐polyclonal antiserum diluted 1:1000 for coating and prepared the pelargonium samples in 0.05 M acetate buffer, pH 6.0 ...
D.‐R. BLYSTAD, V. NAESS, S. HAUGSLIEN
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With the aim of optimizing the detection of pelargonium flower‐break carmovirus in Pelargonium×hortorum by immunosorbent electron microscopy (ISEM), several factors were investigated. The best results were obtained when we used NPPI‐polyclonal antiserum diluted 1:1000 for coating and prepared the pelargonium samples in 0.05 M acetate buffer, pH 6.0 ...
D.‐R. BLYSTAD, V. NAESS, S. HAUGSLIEN
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Plant Pathology, 2006
), a Central Americanornamental, is receiving increasing attention in the USA and Europe forits attractive flowers. In Germany, a number of accessions from com-mercial companies, subjected to evaluation for varietal release, exhibitedconspicuous flower mottling symptoms, suggestive of virus infection.Carmovirus-like isometric particles (approx.
S. Winter +3 more
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), a Central Americanornamental, is receiving increasing attention in the USA and Europe forits attractive flowers. In Germany, a number of accessions from com-mercial companies, subjected to evaluation for varietal release, exhibitedconspicuous flower mottling symptoms, suggestive of virus infection.Carmovirus-like isometric particles (approx.
S. Winter +3 more
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Archives of Virology, 2003
We have studied the biological and molecular characteristics of a MNSV isolate collected in Spain (MNSV-Malpha5) and generated a full-length cDNA clone from which infectious RNA transcripts can be produced. The host range of MNSV-Malpha5 appeared to be limited to cucurbits and did not differ from that of MNSV-Dutch [4, 21].
J A, Díaz +3 more
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We have studied the biological and molecular characteristics of a MNSV isolate collected in Spain (MNSV-Malpha5) and generated a full-length cDNA clone from which infectious RNA transcripts can be produced. The host range of MNSV-Malpha5 appeared to be limited to cucurbits and did not differ from that of MNSV-Dutch [4, 21].
J A, Díaz +3 more
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Journal of Virological Methods, 2006
RNA silencing is a plant defense mechanism in which virus infected plants produce short interfering RNAs (siRNAs) derived from viral RNA, that attack the virus at the post-transcriptional level. In a previous study on Cymbidium ringspot tombusvirus (CymRSV) infection in Nicotiana benthamiana, siRNAs (determined by cloning and sequencing) predominantly ...
Ho, Thien +4 more
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RNA silencing is a plant defense mechanism in which virus infected plants produce short interfering RNAs (siRNAs) derived from viral RNA, that attack the virus at the post-transcriptional level. In a previous study on Cymbidium ringspot tombusvirus (CymRSV) infection in Nicotiana benthamiana, siRNAs (determined by cloning and sequencing) predominantly ...
Ho, Thien +4 more
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Archives of Virology, 2009
The viral genome of soybean yellow mottle mosaic virus (SYMMV) from infected soybean (Glycine max) in Korea was cloned and sequenced. The complete monopartite single-stranded RNA genome of SYMMV consists of 4009 base pairs with six putative open reading frames and includes 5'- and 3'-untranslated regions of 39 and 229 nucleotides, respectively.
Moon, Nam +8 more
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The viral genome of soybean yellow mottle mosaic virus (SYMMV) from infected soybean (Glycine max) in Korea was cloned and sequenced. The complete monopartite single-stranded RNA genome of SYMMV consists of 4009 base pairs with six putative open reading frames and includes 5'- and 3'-untranslated regions of 39 and 229 nucleotides, respectively.
Moon, Nam +8 more
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1998
The 3′-terminal 1500 nucleotides of the genome of pelargonium flower break carmovirus (PFBV) were sequenced from RT-PCR amplification products. One large ORF was found, encoding a 345 amino acid protein of Mr 37 kDa, which corresponds to the coat protein, as confirmed by immunoprecipitation of products of in vitro transcription and translation.
Berthomé, R. +5 more
openaire +2 more sources
The 3′-terminal 1500 nucleotides of the genome of pelargonium flower break carmovirus (PFBV) were sequenced from RT-PCR amplification products. One large ORF was found, encoding a 345 amino acid protein of Mr 37 kDa, which corresponds to the coat protein, as confirmed by immunoprecipitation of products of in vitro transcription and translation.
Berthomé, R. +5 more
openaire +2 more sources