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Prospects for using CRISPR-Cas9 system in the treatment of human viral diseases
Acta Biomedica Scientifica, 2023 The aim. To analyze the possibility of using the genetic mechanisms of CRISPR-Cas9 technology in the prevention and treatment of certain viral diseases.Materials and methods.
A. M. Ziganshin +4 more
doaj +3 more sources
Cas9 Functionally Opens Chromatin. [PDF]
PLoS ONE, 2016 Using a nuclease-dead Cas9 mutant, we show that Cas9 reproducibly induces chromatin accessibility at previously inaccessible genomic loci. Cas9 chromatin opening is sufficient to enable adjacent binding and transcriptional activation by the settler ...
Amira A Barkal +4 more
doaj +6 more sources
Orthogonal Cas9–Cas9 chimeras provide a versatile platform for genome editing [PDF]
Nature Communications, 2018 Therapeutic genome engineering relies on the development of reliable, robust and versatile tools. Here the authors develop Cas9-Cas9 chimeras with high target site activity that generate predictable deletions.
Mehmet Fatih Bolukbasi +8 more
doaj +4 more sources
Development of an RNA aptamer-assisted CRISPR/Cas9 system for efficiently generating and isolating Cas9-free mutants in plant. [PDF]
PLoS GeneticsThe CRISPR/Cas9 gene-editing system is a powerful tool in plant genetic engineering; however, screening for Cas9-free edited plants remains complex and time-consuming.
Sha Liu +10 more
doaj +2 more sources
A review on genome editing by CRISPR-Cas9 technique for cancer treatment [PDF]
World Cancer Research Journal, 2020 In this review, we summarize CRISPR-Cas9 system-based gene modification for the therapeutic treatment of cancer. Cancer is a group of diseases involving anomalous cell growth with the potential to invade or blow out to other parts of the body, which is ...
M. Niuz Morshed Khan +3 more
doaj +1 more source
Gene Editing to Tackle Facioscapulohumeral Muscular Dystrophy
Frontiers in Genome Editing, 2022 Facioscapulohumeral dystrophy (FSHD) is a skeletal muscle disease caused by the aberrant expression of the DUX4 gene in the muscle tissue. To date, different therapeutic approaches have been proposed, targeting DUX4 at the DNA, RNA or protein levels. The
Virginie Mariot, Julie Dumonceaux
doaj +1 more source
Entering the post-epigenomic age: back to epigenetics [PDF]
Open Biology, 2018 It is undeniably one of the greatest findings in biology that (with some very minor exceptions) every cell in the body possesses the whole genetic information needed to generate a complete individual.
Sebastian Bultmann, Stefan H. Stricker
doaj +1 more source
Screening strategy to identify Cas9 variants with higher HDR activity based on diphtheria toxin. [PDF]
J Biomed SciMatsumoto D +6 more
europepmc +2 more sources
CRISPR/CAS9 Technologies [PDF]
Journal of Bone and Mineral Research, 2017 ABSTRACT The Clustered Regularly Interspaced Palindromic Repeats (CRISPR)/CRISPR-associated protein (Cas) pathway is revolutionizing biological research. Modifications to this primitive prokaryotic immune system now enable scientists to efficiently edit DNA or modulate gene expression in living ...
Bart O, Williams, Matthew L, Warman
openaire +2 more sources
Potent CRISPR-Cas9 inhibitors from Staphylococcus genomes. [PDF]
, 2020 Anti-CRISPRs (Acrs) are small proteins that inhibit the RNA-guided DNA targeting activity of CRISPR-Cas enzymes. Encoded by bacteriophage and phage-derived bacterial genes, Acrs prevent CRISPR-mediated inhibition of phage infection and can also block ...
Doudna, Jennifer A +5 more
core +3 more sources