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Orthogonal Cas9–Cas9 chimeras provide a versatile platform for genome editing [PDF]
Nature Communications, 2018 Therapeutic genome engineering relies on the development of reliable, robust and versatile tools. Here the authors develop Cas9-Cas9 chimeras with high target site activity that generate predictable deletions.
Mehmet Fatih Bolukbasi +8 more
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Cas9 Functionally Opens Chromatin. [PDF]
PLoS ONE, 2016 Using a nuclease-dead Cas9 mutant, we show that Cas9 reproducibly induces chromatin accessibility at previously inaccessible genomic loci. Cas9 chromatin opening is sufficient to enable adjacent binding and transcriptional activation by the settler ...
Amira A Barkal +4 more
doaj +7 more sources
A review on genome editing by CRISPR-Cas9 technique for cancer treatment [PDF]
World Cancer Research Journal, 2020 In this review, we summarize CRISPR-Cas9 system-based gene modification for the therapeutic treatment of cancer. Cancer is a group of diseases involving anomalous cell growth with the potential to invade or blow out to other parts of the body, which is ...
M. Niuz Morshed Khan +3 more
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Gene Editing to Tackle Facioscapulohumeral Muscular Dystrophy
Frontiers in Genome Editing, 2022 Facioscapulohumeral dystrophy (FSHD) is a skeletal muscle disease caused by the aberrant expression of the DUX4 gene in the muscle tissue. To date, different therapeutic approaches have been proposed, targeting DUX4 at the DNA, RNA or protein levels. The
Virginie Mariot, Julie Dumonceaux
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CRISPR in dentistry: A boon or bane [PDF]
Microbes and Infectious Diseases, 2023 Background: Clustered Regularly Inter Spaced Palindromic Repeats (CRISPR) is programmable protein that can change the genome's content, remove it, and switch it on and off. This cutting-edge technology offers a wide range of uses and has the potential to
Mirna Garhnayak +3 more
doaj +1 more source
Immunogenicity of Cas9 Protein [PDF]
Journal of Pharmaceutical Sciences, 2020 Clustered regularly interspaced short palindromic repeats (CRISPR) form the adaptive immune system in archaea and bacteria and have been modified for genome engineering in eukaryotic cells. CRISPR systems contain 2 components, a single-guide RNA, which is a short RNA composed of a 20 nucleotide sequence that targets specific sites in the genomic DNA ...
Mehta, Aditi, Merkel, Olivia M.
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CRISPR/Cas9 in Gastrointestinal Malignancies [PDF]
Frontiers in Cell and Developmental Biology, 2021 Gastrointestinal (GI) cancers such as colorectal cancer (CRC), gastric cancer (GC), esophageal cancer (EG), pancreatic duct adenocarcinoma (PDAC) or hepatocellular cancer (HCC) belong to the most commonly diagnosed types of cancer and are among the most frequent causes of cancer related death worldwide.
Jefremow, André +2 more
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Entering the post-epigenomic age: back to epigenetics [PDF]
Open Biology, 2018 It is undeniably one of the greatest findings in biology that (with some very minor exceptions) every cell in the body possesses the whole genetic information needed to generate a complete individual.
Sebastian Bultmann, Stefan H. Stricker
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Journal of Oral Microbiology, 2022 Introduction Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated systems are RNA-mediated adaptive immune systems that actagainst invading genetic elements such as phages or plasmids.
Harumi Mizuki +3 more
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