Results 1 to 10 of about 194,133 (208)
Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells [PDF]
Science, 2014 Improving Whole-Genome Screens Improved methods are needed for the knockout of individual genes in genome-scale functional screens. Wang et al. (p. 80, published online 12 December) and Shalem et al. (p.
Ophir Shalem +10 more
semanticscholar +2 more sources
DNA interrogation by the CRISPR RNA-guided endonuclease Cas9
Nature, 2014 The clustered regularly interspaced short palindromic repeats (CRISPR)-associated enzyme Cas9 is an RNA-guided endonuclease that uses RNA–DNA base-pairing to target foreign DNA in bacteria.
S. H. Sternberg +4 more
semanticscholar +4 more sources
A review on genome editing by CRISPR-Cas9 technique for cancer treatment [PDF]
World Cancer Research Journal, 2020 In this review, we summarize CRISPR-Cas9 system-based gene modification for the therapeutic treatment of cancer. Cancer is a group of diseases involving anomalous cell growth with the potential to invade or blow out to other parts of the body, which is ...
M. Niuz Morshed Khan +3 more
doaj +1 more source
Gene Editing to Tackle Facioscapulohumeral Muscular Dystrophy
Frontiers in Genome Editing, 2022 Facioscapulohumeral dystrophy (FSHD) is a skeletal muscle disease caused by the aberrant expression of the DUX4 gene in the muscle tissue. To date, different therapeutic approaches have been proposed, targeting DUX4 at the DNA, RNA or protein levels. The
Virginie Mariot, Julie Dumonceaux
doaj +1 more source
CRISPR in dentistry: A boon or bane [PDF]
Microbes and Infectious Diseases, 2023 Background: Clustered Regularly Inter Spaced Palindromic Repeats (CRISPR) is programmable protein that can change the genome's content, remove it, and switch it on and off. This cutting-edge technology offers a wide range of uses and has the potential to
Mirna Garhnayak +3 more
doaj +1 more source
Search and localization dynamics of the CRISPR/Cas9 system [PDF]
Phys. Rev. Lett. 127, 208102, 2021, 2021 The CRISPR/Cas9 system acts as the prokaryotic immune system and has important applications in gene editing. The protein Cas9 is one of its crucial components. The role of Cas9 is to search for specific target sequences on the DNA and cleave them. In this Letter, we introduce a model of facilitated diffusion for Cas9 and fit its parameters to single ...
arxiv +1 more source
Entering the post-epigenomic age: back to epigenetics [PDF]
Open Biology, 2018 It is undeniably one of the greatest findings in biology that (with some very minor exceptions) every cell in the body possesses the whole genetic information needed to generate a complete individual.
Sebastian Bultmann, Stefan H. Stricker
doaj +1 more source
CRISPR/Cas9 therapeutics: progress and prospects
Signal Transduction and Targeted Therapy, 2023 Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) gene-editing technology is the ideal tool of the future for treating diseases by permanently correcting deleterious base mutations or disrupting disease-
Tianxiang Li +9 more
semanticscholar +1 more source
Off-target effects in CRISPR/Cas9 gene editing
Frontiers in Bioengineering and Biotechnology, 2023 Gene editing stands for the methods to precisely make changes to a specific nucleic acid sequence. With the recent development of the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system, gene editing has become efficient ...
Congting Guo +3 more
semanticscholar +1 more source
CRISPR-Cas9 In Vivo Gene Editing for Transthyretin Amyloidosis.
New England Journal of Medicine, 2021 BACKGROUND Transthyretin amyloidosis, also called ATTR amyloidosis, is a life-threatening disease characterized by progressive accumulation of misfolded transthyretin (TTR) protein in tissues, predominantly the nerves and heart.
J. Gillmore +25 more
semanticscholar +1 more source