Results 1 to 10 of about 307,709 (381)

Genome editing in potato via CRISPR-Cas9 ribonucleoprotein delivery [PDF]

, 2018
Clustered regularly interspaced short palindromic repeats and CRISPR-associated protein-9 (CRISPR-Cas9) can be used as an efficient tool for genome editing in potato (Solanum tuberosum).
Andersson, Andersson, Cho, Clasen, Eriksson, Gorbunova, Hartung, Jinek, Jones, Kim, Kuipers, Kuipers, Larkin, Liang, Muthoni, Nelson, Sawai, Shepard, Svitashev, Visser, Woo, Xu, Zhang   +22 more
core   +1 more source

Targeted genome modifcation in protoplasts of a highly regenerable Siberian barley cultivar using RNA-guided Cas9 endonuclease

Вавиловский журнал генетики и селекции, 2019
The modifcation of crop genomes employing functional components of the microbial CRISPR/Cas immune system is a rapidly developing area of applied research.
S. V. Gerasimova, A. M. Korotkova, C. Hertig, S. Hiekel, R. Hofe, N. Budhagatapalli, I. Otto, G. Hensel, V. K. Shumny, A. V. Kochetov, J. Kumlehn, E. K. Khlestkina   +11 more
doaj   +1 more source

CRISPOR: intuitive guide selection for CRISPR/Cas9 genome editing experiments and screens

Nucleic Acids Res., 2018
CRISPOR.org is a web tool for genome editing experiments with the CRISPR–Cas9 system. It finds guide RNAs in an input sequence and ranks them according to different scores that evaluate potential off-targets in the genome of interest and predict on ...
J. Concordet, M. Haeussler
semanticscholar   +1 more source

Structural basis for mismatch surveillance by CRISPR–Cas9

Nature, 2022
CRISPR–Cas9 as a programmable genome editing tool is hindered by off-target DNA cleavage1–4, and the underlying mechanisms by which Cas9 recognizes mismatches are poorly understood5–7. Although Cas9 variants with greater discrimination against mismatches
Jack P. K. Bravo, Mu-Sen Liu, Grace N. Hibshman, Tyler L. Dangerfield, Kyungseok Jung, R. S. McCool, K. Johnson, David W. Taylor   +7 more
semanticscholar   +1 more source

Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system. [PDF]

, 2017
Precise genetic modifications in model animals are essential for biomedical research. Here, we report a programmable "base editing" system to induce precise base conversion with high efficiency in zebrafish. Using cytidine deaminase fused to Cas9 nickase,
Bai, Haipeng, Huang, Haigen, Li, Song, Lin, Shuo, Lu, Xiaochan, Qin, Wei, Xu, Jason, Zhang, Yihan   +7 more
core   +1 more source

Off- and on-target effects of genome editing in mouse embryos

The Journal of Reproduction and Development, 2018
Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas-based genome editing technology has enabled manipulation of the embryonic genome.
Shinya AYABE, Kenichi NAKASHIMA, Atsushi YOSHIKI   +2 more
doaj   +1 more source

Exosome-mediated delivery of Cas9 ribonucleoprotein complexes for tissue-specific gene therapy of liver diseases

Science Advances, 2022
CRISPR-Cas9 gene editing has emerged as a powerful therapeutic technology, but the lack of safe and efficient in vivo delivery systems, especially for tissue-specific vectors, limits its broad clinical applications.
Tao Wan, Jiafeng Zhong, Qi Pan, Tianhua Zhou, Y. Ping, Xiangrui Liu   +5 more
semanticscholar   +1 more source

Practical guidance for the implementation of the CRISPR genome editing tool in filamentous fungi [PDF]

, 2019
Background: Within the last years, numerous reports described successful application of the CRISPR nucleases Cas9 and Cpf1 for genome editing in filamentous fungi.
Haefner, Stefan, Kwon, Jin, Meyer, Vera, Schütze, Tabea, Spohner, Sebastian   +4 more
core   +1 more source

A dual conditional CRISPR-Cas9 system to activate gene editing and reduce off-target effects in human stem cells

Molecular Therapy: Nucleic Acids, 2022
The CRISPR-Cas9 system has emerged as a powerful and efficient tool for genome editing. An important drawback of the CRISPR-Cas9 system is the constitutive endonuclease activity when Cas9 endonuclease and its sgRNA are co-expressed.
Seung Bum Park, Takuro Uchida, Samantha Tilson, Zongyi Hu, Christopher D. Ma, Madeleine Leek, Michael Eichner, So Gun Hong, T. Jake Liang   +8 more
doaj   +1 more source

Blood-brain barrier–penetrating single CRISPR-Cas9 nanocapsules for effective and safe glioblastoma gene therapy

Science Advances, 2022
We designed a unique nanocapsule for efficient single CRISPR-Cas9 capsuling, noninvasive brain delivery and tumor cell targeting, demonstrating an effective and safe strategy for glioblastoma gene therapy.
Yan Zou, Xinhong Sun, Qingshan Yang, Meng Zheng, O. Shimoni, W. Ruan, Yibin Wang, Dongya Zhang, Jinlong Yin, Xiangang Huang, Wei Tao, J. B. Park, Xing-jie Liang, Kam W. Leong, B. Shi   +14 more
semanticscholar   +1 more source