Results 31 to 40 of about 278,076 (329)

Creating a tobacco line with a weaker antifeedant property against colorado potato beetle

Биотехнология и селекция растений, 2020
Background. Genetic modification of plants is one of the promising strategies to increase their resistance to insect pests. The development of metabolic or RNA interference systems for plant protection requires appropriate models of host-insect ...
N. E. Kostina, A. V. Spaselnikova, A. A. Egorova, E. V. Kolosovskaya, D. V. Domrachev, A. V. Romanova, S. R. Tumanyan, S. Chamas, J. Kumlehn, I. M. Dubovskiy, S. V. Gerasimova   +10 more
doaj   +1 more source

Computationally designed hyperactive Cas9 enzymes

Nature Communications, 2022
The ability to alter the genomes of living cells is key to understanding how genes influence the functions of organisms and will be critical to modify living systems for useful purposes. Here, the authors use computational design to discover Cas9 enzymes
Pascal D. Vos, Giulia Rossetti, Jessica L. Mantegna, Stefan J. Siira, Andrianto P. Gandadireja, Mitchell Bruce, Samuel A. Raven, Olga Khersonsky, Sarel J. Fleishman, Aleksandra Filipovska, Oliver Rackham   +10 more
doaj   +1 more source

CAS9 is a genome mutator by directly disrupting DNA-PK dependent DNA repair pathway. [PDF]

, 2020
With its high efficiency for site-specific genome editing and easy manipulation, the clustered regularly interspaced short palindromic repeats (CRISPR)/ CRISPR associated protein 9 (CAS9) system has become the most widely used gene editing technology in ...
Chen, Qu, Fu, Xuemei, Kim, Jinchul, Liu, Jingfeng, Tang, Qingshuang, Xu, Shuxiang, Xu, Yang   +6 more
core  

A Newly Discovered Bordetella Species Carries a Transcriptionally Active CRISPR-Cas with a Small Cas9 Endonuclease [PDF]

, 2015
Background Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated genes (cas) are widely distributed among bacteria.
Dudley, Edward G., Harvill, Eric T., Hu, Kai, Ivanov, Yury V., Linz, Bodo, Register, Karen B., Rivera, Israel, Shariat, Nikki   +7 more
core   +2 more sources

Delivering CRISPR: a review of the challenges and approaches

Drug Delivery, 2018
Gene therapy has long held promise to correct a variety of human diseases and defects. Discovery of the Clustered Regularly-Interspaced Short Palindromic Repeats (CRISPR), the mechanism of the CRISPR-based prokaryotic adaptive immune system (CRISPR ...
Christopher A. Lino, Jason C. Harper, James P. Carney, Jerilyn A. Timlin   +3 more
doaj   +1 more source

Off- and on-target effects of genome editing in mouse embryos

The Journal of Reproduction and Development, 2018
Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas-based genome editing technology has enabled manipulation of the embryonic genome.
Shinya AYABE, Kenichi NAKASHIMA, Atsushi YOSHIKI   +2 more
doaj   +1 more source

Nucleolus localization of SpyCas9 affects its stability and interferes with host protein translation in mammalian cells

Genes and Diseases, 2022
The CRISPR/Cas9 system, originally derived from the prokaryotic adaptive immune system, has been developed as efficient genome editing tools. It enables precise gene manipulation on chromosomal DNA through the specific binding of programmable sgRNA to ...
Renke Tan, Wenhao Du, Yiyang Liu, Xiaoji Cong, Meirong Bai, Chenxiao Jiang, Zengxia Li, Minjia Tan, Dengke K. Ma, Qiang Huang, Wei Jiang, Yongjun Dang   +11 more
doaj   +1 more source

An enhanced CRISPR repressor for targeted mammalian gene regulation. [PDF]

, 2018
The RNA-guided endonuclease Cas9 can be converted into a programmable transcriptional repressor, but inefficiencies in target-gene silencing have limited its utility.
Cecchi, Ryan J, Chan, Yingleong, Chavez, Alejandro, Church, George M, Church, George M, Collins, James J, Davidsohn, Noah, Ebrahimkhani, Mo R, Guo, Xiaoge, Kiani, Samira, Kuo, Chih-Chung, Lance-Byrne, Alissa, Lewis, Nathan E, Lim, Elaine T, Menn, David, Milanova, Denitsa, Pradhan, Swechchha, Sharma, Sumana, Tung, Angela, Tuttle, Marcelle, Yeo, Nan Cher   +20 more
core   +1 more source

CRISPR/Cas9 and cancer

Veterinarska stanica, 2021
CRISPR/Cas9 has become a powerful method for making changes to the genome of many organisms. First discovered in bacteria as part of an adaptive immune system, CRISPR/Cas9 and modified versions have found widespread use in genome engineering and in the activation or repression of gen expression.
Mimoune, Nora, Bahouh, Mohamed Wail, Boukhechem, Said, Khelef, Djamel, Kaidi, Rachid, Baazizi, Ratiba   +5 more
openaire   +2 more sources

Practical guidance for the implementation of the CRISPR genome editing tool in filamentous fungi [PDF]

, 2019
Background: Within the last years, numerous reports described successful application of the CRISPR nucleases Cas9 and Cpf1 for genome editing in filamentous fungi.
Haefner, Stefan, Kwon, Jin, Meyer, Vera, Schütze, Tabea, Spohner, Sebastian   +4 more
core   +1 more source