Results 41 to 50 of about 278,076 (329)

Coupling Cas9 to artificial inhibitory domains enhances CRISPR-Cas9 target specificity [PDF]

Science Advances, 2020
Fusing attenuated anti-CRISPR proteins to Cas9 improves specificity via kinetic insulation of ON- and OFF-target editing events.
Sabine Aschenbrenner, Stefan M. Kallenberger, Mareike D. Hoffmann, Adrian Huck, Roland Eils, Dominik Niopek   +5 more
openaire   +2 more sources

Concerning RNA-guided gene drives for the alteration of wild populations

eLife, 2014
Gene drives may be capable of addressing ecological problems by altering entire populations of wild organisms, but their use has remained largely theoretical due to technical constraints. Here we consider the potential for RNA-guided gene drives based on
Kevin M Esvelt, Andrea L Smidler, Flaminia Catteruccia, George M Church   +3 more
doaj   +1 more source

A dual conditional CRISPR-Cas9 system to activate gene editing and reduce off-target effects in human stem cells

Molecular Therapy: Nucleic Acids, 2022
The CRISPR-Cas9 system has emerged as a powerful and efficient tool for genome editing. An important drawback of the CRISPR-Cas9 system is the constitutive endonuclease activity when Cas9 endonuclease and its sgRNA are co-expressed.
Seung Bum Park, Takuro Uchida, Samantha Tilson, Zongyi Hu, Christopher D. Ma, Madeleine Leek, Michael Eichner, So Gun Hong, T. Jake Liang   +8 more
doaj   +1 more source

CRISPR/Cas9 mediated knockout of rb1 and rbl1 leads to rapid and penetrant retinoblastoma development in Xenopus tropicalis [PDF]

, 2016
Retinoblastoma is a pediatric eye tumor in which bi-allelic inactivation of the Retinoblastoma 1 (RB1) gene is the initiating genetic lesion. Although recently curative rates of retinoblastoma have increased, there are at this time no molecular targeted ...
Boel, Annekatrien, Colpaert, Robin, Creytens, David, Deforce, Dieter, Dimitrakopoulou, Dionysia, Haustraete, Jurgen, L, J, Lepez, Trees, Naert, Thomas, Steyaert, Wouter, Van Nieuwenhuysen, Tom, Vleminckx, Kris, Willaert, Andy   +12 more
core   +2 more sources

Genome editing in potato via CRISPR-Cas9 ribonucleoprotein delivery [PDF]

, 2018
Clustered regularly interspaced short palindromic repeats and CRISPR-associated protein-9 (CRISPR-Cas9) can be used as an efficient tool for genome editing in potato (Solanum tuberosum).
Andersson, Andersson, Cho, Clasen, Eriksson, Gorbunova, Hartung, Jinek, Jones, Kim, Kuipers, Kuipers, Larkin, Liang, Muthoni, Nelson, Sawai, Shepard, Svitashev, Visser, Woo, Xu, Zhang   +22 more
core   +1 more source

Interplay between circadian and other transcription factors—Implications for cycling transcriptome reprogramming

FEBS Letters, EarlyView.
This perspective highlights emerging insights into how the circadian transcription factor CLOCK:BMAL1 regulates chromatin architecture, cooperates with other transcription factors, and coordinates enhancer dynamics. We propose an updated framework for how circadian transcription factors operate within dynamic and multifactorial chromatin landscapes ...
Xinyu Y. Nie, Jerome S. Menet
wiley   +1 more source

Current Applications and Future Perspectives of CRISPR-Cas9 for the Treatment of Lung Cancer

Biologics: Targets & Therapy, 2021
Markeshaw Tiruneh G/Medhin,1 Endeshaw Chekol Abebe,2 Tekeba Sisay,3 Nega Berhane,3 Tesfahun Bekele Snr,1 Tadesse Asmamaw Dejenie1 1Department of Biochemistry, School of Medicine, College of Medicine and Health Sciences, University of Gondar, Gondar ...
Tiruneh G/Medhin M, Chekol Abebe E, Sisay T, Berhane N, Bekele Snr T, Asmamaw Dejenie T   +5 more
doaj  

Targeted genome modifcation in protoplasts of a highly regenerable Siberian barley cultivar using RNA-guided Cas9 endonuclease

Вавиловский журнал генетики и селекции, 2019
The modifcation of crop genomes employing functional components of the microbial CRISPR/Cas immune system is a rapidly developing area of applied research.
S. V. Gerasimova, A. M. Korotkova, C. Hertig, S. Hiekel, R. Hofe, N. Budhagatapalli, I. Otto, G. Hensel, V. K. Shumny, A. V. Kochetov, J. Kumlehn, E. K. Khlestkina   +11 more
doaj   +1 more source

A CRISPR-Cas9 sex-ratio distortion system for genetic control. [PDF]

, 2016
Genetic control aims to reduce the ability of insect pest populations to cause harm via the release of modified insects. One strategy is to bias the reproductive sex ratio towards males so that a population decreases in size or is eliminated altogether ...
Bernardini, F, Crisanti, A, Galizi, R, Hammond, A, Kyrou, K, Nolan, T, O'Loughlin, SM, Papathanos, PA, Taxiarchi, C, Windbichler, N   +9 more
core   +3 more sources

Modeling hepatic fibrosis in TP53 knockout iPSC‐derived human liver organoids

Molecular Oncology, EarlyView.
This study developed iPSC‐derived human liver organoids with TP53 gene knockout to model human liver fibrosis. These organoids showed elevated myofibroblast activation, early disease markers, and advanced fibrotic hallmarks. The use of profibrotic differentiation medium further amplified the fibrotic signature seen in the organoids.
Mustafa Karabicici, Soheil Akbari, Ceyda Caliskan, Canan Celiker, Ozden Oz, Leman Binokay, Gökhan Karakulah, Serif Senturk, Esra Erdal   +8 more
wiley   +1 more source