Results 51 to 60 of about 194,133 (208)
Proceedings of the National Academy of Sciences of the United States of America, 2012 Clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems provide adaptive immunity against viruses and plasmids in bacteria and archaea.
G. Gasiunas +3 more
semanticscholar +1 more source
Proceedings of the National Academy of Sciences of the United States of America, 2021 Significance Genome editing technologies enable the permanent repair of disease-causing genetic mutations. However, the application of this technology has been limited by the technical challenge of achieving safe, effective, and specific in vivo delivery
Min Qiu +10 more
semanticscholar +1 more source
Current Applications and Future Perspectives of CRISPR-Cas9 for the Treatment of Lung Cancer
Biologics: Targets & Therapy, 2021 Markeshaw Tiruneh G/Medhin,1 Endeshaw Chekol Abebe,2 Tekeba Sisay,3 Nega Berhane,3 Tesfahun Bekele Snr,1 Tadesse Asmamaw Dejenie1 1Department of Biochemistry, School of Medicine, College of Medicine and Health Sciences, University of Gondar, Gondar ...
Tiruneh G/Medhin M +5 more
doaj
CRISPR–Cas9 Based Bacteriophage Genome Editing
Microbiology Spectrum, 2022 Bacteriophages are the most abundant entities in the biosphere, and many genomes of rare and novel bacteriophages have been sequenced to date. However, bacteriophage functional genomics has been limited by a lack of effective research methods.
Xueli Zhang +5 more
doaj +1 more source
CRISPR-Cas9 Structures and Mechanisms.
Annual Review of Biophysics, 2017 Many bacterial clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated (Cas) systems employ the dual RNA-guided DNA endonuclease Cas9 to defend against invading phages and conjugative plasmids by introducing site-specific ...
F. Jiang, J. Doudna
semanticscholar +1 more source
CRISPR-Cas9 genome editing using targeted lipid nanoparticles for cancer therapy
Science Advances, 2020 CRISPR lipid nanoparticles promote in vivo therapeutic genome editing in two aggressive cancer mouse models. Harnessing CRISPR-Cas9 technology for cancer therapeutics has been hampered by low editing efficiency in tumors and potential toxicity of ...
Daniel Rosenblum +11 more
semanticscholar +1 more source
Efficient Multiple Genome Modifications Induced by the crRNAs, tracrRNA and Cas9 Protein Complex in Zebrafish. [PDF]
PLoS ONE, 2015 The type II clustered regularly interspaced short palindromic repeats (CRISPR) associated with Cas9 endonuclease (CRISPR/Cas9) has become a powerful genetic tool for understanding the function of a gene of interest.
Hirohito Kotani +4 more
doaj +1 more source
R-loop formation and conformational activation mechanisms of Cas9
Nature, 2022 Cas9 is a CRISPR-associated endonuclease capable of RNA-guided, site-specific DNA cleavage1–3. The programmable activity of Cas9 has been widely utilized for genome editing applications4–6, yet its precise mechanisms of target DNA binding and off-target ...
Martin Pacesa +5 more
semanticscholar +1 more source
Genome Biology, 2022 Background Due to post-cleavage residence of the Cas9-sgRNA complex at its target, Cas9-induced DNA double-strand breaks (DSBs) have to be exposed to engage DSB repair pathways.
Si-Cheng Liu +16 more
doaj +1 more source
Prioritization of cancer therapeutic targets using CRISPR–Cas9 screens
Nature, 2019 Functional genomics approaches can overcome limitations—such as the lack of identification of robust targets and poor clinical efficacy—that hamper cancer drug development. Here we performed genome-scale CRISPR–Cas9 screens in 324 human cancer cell lines
F. Behan +24 more
semanticscholar +1 more source