Results 61 to 70 of about 194,133 (208)

Orthogonal Cas9–Cas9 chimeras provide a versatile platform for genome editing

Nature Communications, 2018
Therapeutic genome engineering relies on the development of reliable, robust and versatile tools. Here the authors develop Cas9-Cas9 chimeras with high target site activity that generate predictable deletions.
Mehmet Fatih Bolukbasi, Pengpeng Liu, Kevin Luk, Samantha F. Kwok, Ankit Gupta, Nadia Amrani, Erik J. Sontheimer, Lihua Julie Zhu, Scot A. Wolfe   +8 more
doaj   +1 more source

Epigenome editing by a CRISPR/Cas9-based acetyltransferase activates genes from promoters and enhancers

Nature Biotechnology, 2015
Technologies that enable targeted manipulation of epigenetic marks could be used to precisely control cell phenotype or interrogate the relationship between the epigenome and transcriptional control.
Isaac B. Hilton, A. D'Ippolito, Christopher M. Vockley, P. Thakore, G. Crawford, Timothy E. Reddy, C. Gersbach   +6 more
semanticscholar   +1 more source

Evaluation and Minimization of Cas9-Independent Off-Target DNA Editing by Cytosine Base Editors

Nature Biotechnology, 2020
Cytosine base editors (CBEs) enable targeted C•G-to-T•A conversions in genomic DNA. Recent studies report that BE3, the original CBE, induces a low frequency of genome-wide Cas9-independent off-target C•G-to-T•A mutation in mouse embryos and in rice ...
Jordan L. Doman, Aditya Raguram, Gregory A. Newby, David R. Liu   +3 more
semanticscholar   +1 more source

Comparison of CRISPR-Cas9/Cas12a Ribonucleoprotein Complexes for Genome Editing Efficiency in the Rice Phytoene Desaturase (OsPDS) Gene

Rice, 2020
Background Delivery of CRISPR reagents into cells as ribonucleoprotein (RNP) complexes enables transient editing, and avoids CRISPR reagent integration in the genomes.
Raviraj Banakar, Mollie Schubert, Michael Collingwood, Christopher Vakulskas, Alan L. Eggenberger, Kan Wang   +5 more
doaj   +1 more source

MAGeCK enables robust identification of essential genes from genome-scale CRISPR/Cas9 knockout screens

Genome Biology, 2014
We propose the Model-based Analysis of Genome-wide CRISPR/Cas9 Knockout (MAGeCK) method for prioritizing single-guide RNAs, genes and pathways in genome-scale CRISPR/Cas9 knockout screens.
Wei Li, Han Xu, T. Xiao, Le Cong, Le Cong, M. Love, Feng Zhang, Feng Zhang, R. Irizarry, Jun S. Liu, Myles Brown, Myles A. Brown, X. Liu   +12 more
semanticscholar   +1 more source

Comparison of Various Nuclear Localization Signal-Fused Cas9 Proteins and Cas9 mRNA for Genome Editing in Zebrafish

G3: Genes, Genomes, Genetics, 2018
The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system has been proven to be an efficient and precise genome editing technology in various organisms.
Peinan Hu, Xueying Zhao, Qinghua Zhang, Weiming Li, Yao Zu   +4 more
doaj   +1 more source

Minimal 2'-O-methyl phosphorothioate linkage modification pattern of synthetic guide RNAs for increased stability and efficient CRISPR-Cas9 gene editing avoiding cellular toxicity. [PDF]

PLoS ONE, 2017
Since its initial application in mammalian cells, CRISPR-Cas9 has rapidly become a preferred method for genome engineering experiments. The Cas9 nuclease is targeted to genomic DNA using guide RNAs (gRNA), either as the native dual RNA system consisting ...
Megan Basila, Melissa L Kelley, Anja van Brabant Smith   +2 more
doaj   +1 more source

High-fidelity CRISPR-Cas9 variants with undetectable genome-wide off-targets

Nature, 2015
CRISPR–Cas9 nucleases are widely used for genome editing but can induce unwanted off-target mutations. Existing strategies for reducing genome-wide off-target effects of the widely used Streptococcus pyogenes Cas9 (SpCas9) are imperfect, possessing only ...
B. Kleinstiver, V. Pattanayak, Michelle S. Prew, S. Tsai, Nhu T. Nguyen, Zongli Zheng, J. Joung   +6 more
semanticscholar   +1 more source

Nucleolus localization of SpyCas9 affects its stability and interferes with host protein translation in mammalian cells

Genes and Diseases, 2022
The CRISPR/Cas9 system, originally derived from the prokaryotic adaptive immune system, has been developed as efficient genome editing tools. It enables precise gene manipulation on chromosomal DNA through the specific binding of programmable sgRNA to ...
Renke Tan, Wenhao Du, Yiyang Liu, Xiaoji Cong, Meirong Bai, Chenxiao Jiang, Zengxia Li, Minjia Tan, Dengke K. Ma, Qiang Huang, Wei Jiang, Yongjun Dang   +11 more
doaj  

CRISPR/Cas9 For Photoactivated Localization Microscopy (PALM) [PDF]

arXiv, 2014
We demonstrate that endonuclease deficient Clustered Regularly Interspaced Short Palindromic Repeats CRISPR-associated Cas9 protein (dCas9) fused to the photo-convertible fluorescence protein monomeric mEos3.1 (dCas9-mEos3) can be used to resolve sub-diffraction limited features of repetitive gene elements, thus providing a new route to investigate ...
arxiv