Results 61 to 70 of about 17,756 (247)

High‐Dimensional Spectral Flow Cytometry Panel for Immunophenotypic Characterization of Fixed Whole Blood Samples

Cytometry Part A, EarlyView.
ABSTRACT Peripheral blood mononuclear cells (PBMCs) are commonly used for immune profiling in clinical research, but their isolation and cryopreservation present logistical challenges, particularly in settings with limited access to research facilities. To address this, we implemented a whole blood fixation protocol to preserve surface protein epitopes
Vaishnavi Parthasarathy, Alexander T. Heubeck, Tyanna Stuckey, Veronica Hernandez, Blessing Musgrove, Maia M. Bennett‐Boehm, Troy Torgerson, Peter Skene, Julian Reading   +8 more
wiley   +1 more source

Valproic acid increases CAR T cell cytotoxicity against acute myeloid leukemia

Journal for ImmunoTherapy of Cancer, 2023
The treatment of B cell malignancies has dramatically changed with the introduction of immunotherapy, especially chimeric antigen receptor T (CAR-T) cell therapy. However, only limited efficacy is observed in acute myeloid leukaemia (AML).
Ting Yang, Jingjing Wen, Yanxin Chen, Jiajie Yang, Chunye Dai, Shenjie Yu, Wenting Zhong, Lilin Liu, Chengguanng He, Wenmin Zhang, Lingfeng Liu, Jianda Hu   +11 more
doaj   +1 more source

CD33-CD123 IF-THEN gating reduces toxicity while enhancing the specificity and memory phenotype of AML-targeting CAR-T cells.

Blood Cancer Discovery
CAR T-cell therapy has remarkably succeeded in treating lymphoblastic leukemia. However, its success in AML remains elusive due to the risk of on-target off-tumor toxicity to hematopoietic stem and progenitor cells (HSPC) and insufficient T-cell ...
S. Jambon, Jian-Ping Sun, Shawn Barman, Sakunthala Muthugounder, Xue Rachel Bito, A. Shadfar, Alexandra E. Kovach, B. Wood, Varsha Thoppey Manoharan, A. Morrissy, D. Bhojwani, A. Wayne, M. Pulsipher, Yong-Mi Kim, S. Asgharzadeh, C. Parekh, Babak Moghimi   +16 more
semanticscholar   +1 more source

First‐in‐human study of JNJ‐63709178, a CD123/CD3 targeting antibody, in relapsed/refractory acute myeloid leukemia

Clinical and Translational Science, 2022
This study aimed to identify a recommended phase II dose and evaluate the safety, tolerability, pharmacokinetics/pharmacodynamics, and preliminary clinical activity of JNJ‐63709178, a CD123/CD3 dual‐targeting antibody, in patients with relapsed or ...
M. Boyiadzis, Pinkal M. Desai, N. Daskalakis, W. Donnellan, Lucille Ferrante, J. Goldberg, M. Grunwald, Christina D Guttke, Xiang Li, J. Pérez-Simón, O. Salamero, T. Tucker, Xiaoying Xu, Jay Yang, Naveen Pemmaraju, J. Alonso-Domínguez   +15 more
semanticscholar   +1 more source

Flow cytometry‐based monitoring of chimeric antigen receptor (CAR) T cells: Reagent selection, assay design, and clinical utility

Cytometry Part B: Clinical Cytometry, EarlyView.
Abstract Accurate quantification of chimeric antigen receptor (CAR) T cells is essential for monitoring post‐infusion CART expansion and persistence and for real‐time clinical decision‐making. Multiparameter flow cytometry (MFC) enables rapid, live‐cell detection with absolute quantification and concurrent immunophenotypic characterization. This review
Jianhua Ling, Wei Wang, Sa A. Wang
wiley   +1 more source

Umbilical Cord Blood as a Source of Less Differentiated T Cells to Produce CD123 CAR-T Cells

Cancers, 2022
Simple Summary We used fresh or thawed Umbilical Cord Blood (UCB) to produce CAR-T cells directed against CD123, and we compared their functionality to Peripheral Blood (PB) CAR-T cells.
B. Caël, J. Galaine, I. Bardey, C. Marton, Maxime Fredon, S. Biichlé, Margaux Poussard, Y. Godet, Fanny Angelot-Delettre, C. Barisien, Christophe Bésiers, O. Adotévi, F. Pouthier, F. Garnache-Ottou, E. Bôle-Richard   +14 more
semanticscholar   +1 more source

Advances in γδ T Cells: Cutaneous Biology and Therapeutic Potential for Skin Neoplasms

The Journal of Dermatology, EarlyView.
ABSTRACT γδ T cells represent an increasingly important subset of T lymphocytes in the immune response to cancer and as a platform for novel immunotherapies. The aim of this review is to synthesize recent insights into the biology and advances in therapeutic applications of γδ T cells in cutaneous malignancies, especially melanoma, within a framework ...
Simo Du, Anika Jallorina, Toru Yoshino, Lingyu Bao, Han Bao, Victor Chang, Henry K. Wong, Derek V. Chan, Stephen K. Richardson, Mary F. Lee‐Wong   +9 more
wiley   +1 more source

Diagnostic Challenges of CD4+/CD56+/CD123+ hematological neoplasms [PDF]

Anais Brasileiros de Dermatologia, 2013
In the January/February 2013 edition, Maio et al describe a patient with CD4/CD56/CD123 ascribed as blastic plasmacytoid dendritic cell neoplasia (BDCN). However, since CD4/CD56/CD123 neoplasms are highly heterogeneous, the precise diagnosis requires an extensive immunophenotypic panel. 3 Although highly suggestive, the cytochemical positivity for CD4,
Thiago, Leandro S., Sandes, Alex Freire
openaire   +4 more sources

Optimising the Diagnosis of Sesame Seed Allergy With the Basophil Activation Test

Allergy, EarlyView.
The basophil activation test (using the activation marker CD203c) was the only test able to both exclude and confirm sesame seed allergy with precision. A negative skin prick test to sesame extract or tahini and undetectable IgE to Ses i 1 could not rule out sesame seed allergy.
Ru‐Xin Foong, Irene Bartha, Holly Boyd, Andreina Marques‐Mejias, Suzana Radulovic, Marta Krawiec, Faye Harrison, Malak Eghleilib, Grammatiki Antoneria, Zainab Jama, Matthew Kwok, Vasco Claro, Cristian Ricci, Tom Marrs, Gideon Lack, George Du Toit, Alexandra F. Santos   +16 more
wiley   +1 more source

Kinetics of Antibody Responses and Effector Cell Sensitivity After High Dose Birch Extract Nasal Challenge

Allergy, EarlyView.
High‐dose birch‐extract nasal challenge induced synchronous peaks at 4 weeks in serum specific IgE (sIgE) and basophil sensitivity. Mast cell sensitivity via tSPT and sIgG1 and sIgG4 responses peaked later, at 6–8 weeks. Preseasonal allergen exposure attenuated seasonal sIgE increase and prevented further rise in tSPT sensitivity.
N. J. Campion, S. Villazala‐Merino, C. Morgenstern, L. Nemec, V. Stanek, A. Tu, M. Zghaebi, J. Toth, R. Fröschl, T. Perkmann, M. Bastl, K. Gangl, S. Schneider, H. Breiteneder, R. Ristl, M. Focke‐Tejkl, R. Valenta, V. Niederberger‐Leppin, J. Eckl‐Dorna   +18 more
wiley   +1 more source