Results 131 to 140 of about 113,609 (263)

Flow cytometry‐based monitoring of chimeric antigen receptor (CAR) T cells: Reagent selection, assay design, and clinical utility

Cytometry Part B: Clinical Cytometry, EarlyView.
Abstract Accurate quantification of chimeric antigen receptor (CAR) T cells is essential for monitoring post‐infusion CART expansion and persistence and for real‐time clinical decision‐making. Multiparameter flow cytometry (MFC) enables rapid, live‐cell detection with absolute quantification and concurrent immunophenotypic characterization. This review
Jianhua Ling, Wei Wang, Sa A. Wang
wiley   +1 more source

B7 homolog 3-targeted CAR-T cells secreting EGFR T-cell engagers for improved control of glioblastoma progression. [PDF]

Mol Biomed
Zhang Z, Yang N, Zeng H, Chen Y, Lu H, Xu L, Wang Z, Wang G, Zhou L, Tong A.   +9 more
europepmc   +1 more source

Establishment of pediatric reference ranges for circulating naïve and memory T and B cell subsets guided by the human immunophenotyping consortium standardization initiative: A large, single center U.S. experience

Cytometry Part B: Clinical Cytometry, EarlyView.
Abstract Our knowledge of the immune system continues to expand at a rapid pace, and this coupled with technological advances now enables us to interrogate both the breadth and the depth of the immune response at levels without precedent. This has also facilitated rapidly integrating some of this carefully vetted knowledge into clinical practice ...
Aaruni Khanolkar, Aisha Ahmed
wiley   +1 more source

Human CART22.19 therapy in refractory pediatric B-ALL: insights from a named-patient cohort. [PDF]

J Immunother Cancer
Mast AS, Lang P, Schlegel P, Calkoen FG, Atar D, Scheuermann S, Klein S, Braun C, Schinle F, Schmidt M, Hensen L, Ebinger M, Döring M, Schäfer JF, Schulte J, Bethge WA, Lengerke C, Alahmari B, Hu P, Schneider D, Orentas RJ, Handgretinger R, Seitz CM.   +22 more
europepmc   +1 more source

DinoFlow: Self‐supervised pretraining in flow cytometry enables accurate detection of common hematopathological disorders

Cytometry Part B: Clinical Cytometry, EarlyView.
Abstract Flow cytometry is an essential component of routine hematological lab testing. Many computational methods have been proposed for the analysis of flow cytometry data, but most have focused on supervised learning for just one or a few specific disorders.
Brendan O'Fallon, Muir Morrison, Mattia Medina Grespan, Nicholas C. Spies, David P. Ng   +4 more
wiley   +1 more source

APOBEC2 deficiency disrupts hematopoietic lineage commitment, resulting in emergence of dual identity lymphocytes in mice and humans. [PDF]

Proc Natl Acad Sci U S A
Moris Amro E, Gkougkousis C, Seebass JG, Tellioglu I, Bakr A, Lorenzo JP, Abolhassani H, Altenhofen J, Berntson L, Ruf S, Hammarström L, Pan-Hammarström Q, Imbusch CD, Papavasiliou FN.   +13 more
europepmc   +1 more source

Optimizing Rituximab Maintenance Therapy: Outcomes of Extended-Interval Dosing in Multiple Sclerosis and Neuromyelitis Optica Spectrum Disorder. [PDF]

J Clin Med Res
Kittipadakul S, Ongphichetmetha T, Siritho S, Uawithya E, Prayoonwiwat N, Rattanathamsakul N, Jitprapaikulsan J.   +6 more
europepmc   +1 more source

Automated B‐cell and plasma cell identification using unsupervised clustering by FlowSOM and an excel‐based classification model

Cytometry Part B: Clinical Cytometry, EarlyView.
Abstract Manual gating for plasma cell (PC) identification in multiparametric flow cytometry (MFC) is time‐consuming and operator‐dependent, especially when PCs are scarce. Artificial intelligence approaches such as unsupervised clustering (e.g., FlowSOM) map high‐dimensional data that still require expert interpretation.
Ethan James Gantana, Ernest Musekwa, Erica‐Mari Nell, Zivanai Cuthbert Chapanduka   +3 more
wiley   +1 more source

Deep phenotyping of skin tissue remodeling in patients with systemic sclerosis treated with CD19-CAR T cells. [PDF]

Nat Commun
Rius Rigau A, Xu M, Liu Z, Chenguiti Fakhouri S, Auth J, Garantziotis P, Zoli A, Selvaraju MK, Raimondo MG, Tur C, Filla T, Gehringer P, Eckstein M, Müller F, Atzinger A, Ronicke M, Ekici A, Schmid R, Wirsching A, Hagen M, Böltz S, Krickau T, Horch RE, Berking C, Grieshaber-Bouyer R, Ramming A, Gupta P, Bozec A, Mackensen A, Distler JH, Schett G, Li YN, Bergmann C.   +32 more
europepmc   +1 more source

Extended routine lymphocyte immunophenotyping refines individual immune organization across a heterogeneous patient cohort

Cytometry Part B: Clinical Cytometry, EarlyView.
Abstract Routine TBNK immunophenotyping detects the major lymphocyte compartments but may underestimate the biologically relevant variation contributed by less frequent but well‐defined minor lymphocyte subsets. This study evaluated whether extending routine TBNK immunophenotyping with minor lymphocyte subsets provides complementary, analytically ...
Carlos Agustin Villegas Valverde, Yendry Ventura Carmenate, Vianed Marsan Suarez, Yandy Marx Castillo Aleman, Imilla Casado Hernandez, Rachely Hernandez Corrales   +5 more
wiley   +1 more source